This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (40) Citing Articles via Google Scholar Google Scholar Articles by Balhadère, P. V. Articles by Talbot, N. J. Search for Related Content PubMed PubMed Citation Articles by Balhadère, P. V. Articles by Talbot, N. J. Agricola Articles by Balhadère, P. V. Articles by Talbot, N. J.

PDE1 Encodes a P-Type ATPase Involved in Appressorium-Mediated Plant Infection by the Rice Blast Fungus Magnaporthe grisea

School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, United Kingdom

¹ To whom correspondence should be addressed. E-mail n.j.talbot{at}exeter.ac.uk; fax 1392-264668

Plant infection by the rice blast fungus Magnaporthe grisea is brought about by the action of specialized infection cells called appressoria. These infection cells generate enormous turgor pressure, which is translated into an invasive force that allows a narrow penetration hypha to breach the plant cuticle. The Magnaporthe pde1 mutant was identified previously by restriction enzyme–mediated DNA integration mutagenesis and is impaired in its ability to elaborate penetration hyphae. Here we report that the pde1 mutation is the result of an insertion into the promoter of a P-type ATPase-encoding gene. Targeted gene disruption confirmed the role of PDE1 in penetration hypha development and pathogenicity but highlighted potential differences in PDE1 regulation in different Magnaporthe strains. The predicted PDE1 gene product was most similar to members of the aminophospholipid translocase group of P-type ATPases and was shown to be a functional homolog of the yeast ATPase gene ATC8. Spatial expression studies showed that PDE1 is expressed in germinating conidia and developing appressoria. These findings implicate the action of aminophospholipid translocases in the development of penetration hyphae and the proliferation of the fungus beyond colonization of the first epidermal cell.

This article has been cited by other articles:

				G. Hu and J. W. Kronstad A Putative P-Type ATPase, Apt1, Is Involved in Stress Tolerance and Virulence in Cryptococcus neoformans Eukaryot. Cell, January 1, 2010; 9(1): 74 - 83. [Abstract] [Full Text] [PDF]

				I. Fudal, J. Collemare, H. U. Bohnert, D. Melayah, and M.-H. Lebrun Expression of Magnaporthe grisea Avirulence Gene ACE1 Is Connected to the Initiation of Appressorium-Mediated Penetration Eukaryot. Cell, March 1, 2007; 6(3): 546 - 554. [Abstract] [Full Text] [PDF]

				K. Liu, Z. Hua, J. A. Nepute, and T. R. Graham Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway Mol. Biol. Cell, February 1, 2007; 18(2): 487 - 500. [Abstract] [Full Text] [PDF]

				A. C. Sexton and B. J. Howlett Parallels in Fungal Pathogenesis on Plant and Animal Hosts Eukaryot. Cell, December 1, 2006; 5(12): 1941 - 1949. [Full Text] [PDF]

				Z. Hua, P. Fatheddin, and T. R. Graham An Essential Subfamily of Drs2p-related P-Type ATPases Is Required for Protein Trafficking between Golgi Complex and Endosomal/Vacuolar System Mol. Biol. Cell, September 1, 2002; 13(9): 3162 - 3177. [Abstract] [Full Text] [PDF]