Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (102)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gardiner, J. C.
Right arrow Articles by Marc, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gardiner, J. C.
Right arrow Articles by Marc, J.
Agricola
Right arrow Articles by Gardiner, J. C.
Right arrow Articles by Marc, J.
The Plant Cell, Vol. 13, 2143-2158, September 2001, Copyright © 2001,
American Society of Plant Biologists

A 90-kD Phospholipase D from Tobacco Binds to Microtubules and the Plasma Membrane

John C. Gardinera, John D. I. Harpera, Nimalika D. Weerakoona, David A. Collingsa, Sian Ritchieb, Simon Gilroyb, Richard J. Cyrb and Jan Marc1,a

a School of Biological Sciences, Macleay Building A12, University of Sydney, Sydney 2006, Australia
b Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802

1 To whom correspondence should be addressed. E-mail jmarc{at}bio.usyd.edu.au; fax 61-2-9351-4771

The organization of microtubule arrays in the plant cell cortex involves interactions with the plasma membrane, presumably through protein bridges. We have used immunochemistry and monoclonal antibody 6G5 against a candidate bridge protein, a 90-kD tubulin binding protein (p90) from tobacco BY-2 membranes, to characterize the protein and isolate the corresponding gene. Screening an Arabidopsis cDNA expression library with the antibody 6G5 produced a partial clone encoding phospholipase D (PLD), and a full-length gene was obtained by sequencing a corresponding expressed sequence tag clone. The predicted protein of 857 amino acids contains the active sites of a phospholipid-metabolizing enzyme and a Ca2+-dependent lipid binding domain and is identical to Arabidopsis PLD{delta}. Two amino acid sequences obtained by Edman degradation of the tobacco p90 are identical to corresponding segments of a PLD sequence from tobacco. Moreover, immunoprecipitation using the antibody 6G5 and tobacco BY-2 protein extracts gave significant PLD activity, and PLD activity of tobacco BY-2 membrane proteins was enriched 6.7-fold by tubulin-affinity chromatography. In a cosedimentation assay, p90 bound and decorated microtubules. In immunofluorescence microscopy of intact tobacco BY-2 cells or lysed protoplasts, p90 colocalized with cortical microtubules, and taxol-induced microtubule bundling was accompanied by corresponding reorganization of p90. Labeling of p90 remained along the plasma membrane when microtubules were depolymerized, although detergent extraction abolished the labeling. Therefore, p90 is a specialized PLD that associates with membranes and microtubules, possibly conveying hormonal and environmental signals to the microtubule cytoskeleton.




This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Cai
Assembly and disassembly of plant microtubules: tubulin modifications and binding to MAPs
J. Exp. Bot., March 1, 2010; 61(3): 623 - 626.
[Full Text] [PDF]

Home page
 J Exp BotHome page
L. Li, N. Saga, and K. Mikami
Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis
J. Exp. Bot., August 1, 2009; 60(12): 3477 - 3489.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. Guo, C.-M. K. Ho, Z. Kong, Y.-R. J. Lee, Q. Qian, and B. Liu
Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome
Ann. Bot., February 1, 2009; 103(3): 387 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
A. Driouich and T. I. Baskin
Intercourse between cell wall and cytoplasm exemplified by arabinogalactan proteins and cortical microtubules
Am. J. Botany, December 1, 2008; 95(12): 1491 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. P. Smertenko, D. Kaloriti, H.-Y. Chang, J. Fiserova, Z. Opatrny, and P. J. Hussey
The C-Terminal Variable Region Specifies the Dynamic Properties of Arabidopsis Microtubule-Associated Protein MAP65 Isotypes
PLANT CELL, December 1, 2008; 20(12): 3346 - 3358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Ambrose and G. O. Wasteneys
CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules
Mol. Biol. Cell, November 1, 2008; 19(11): 4730 - 4737.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Paredez, S. Persson, D. W. Ehrhardt, and C. R. Somerville
Genetic Evidence That Cellulose Synthase Activity Influences Microtubule Cortical Array Organization
Plant Physiology, August 1, 2008; 147(4): 1723 - 1734.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. A. Barton, M. Vantard, and R. L. Overall
Analysis of Cortical Arrays from Tradescantia virginiana at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading
PLANT CELL, April 1, 2008; 20(4): 982 - 994.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Li, R. Wang, M. Li, L. Li, C. Wang, R. Welti, and X. Wang
Differential Degradation of Extraplastidic and Plastidic Lipids during Freezing and Post-freezing Recovery in Arabidopsis thaliana
J. Biol. Chem., January 4, 2008; 283(1): 461 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. T. Peters, K. O. Logan, A. C. Miller, and D. L. Kropf
Phospholipase D Signaling Regulates Microtubule Organization in the Fucoid Alga Silvetia compressa
Plant Cell Physiol., December 1, 2007; 48(12): 1764 - 1774.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Langhans and D. G. Robinson
1-Butanol targets the Golgi apparatus in tobacco BY-2 cells, but in a different way to Brefeldin A
J. Exp. Bot., September 1, 2007; 58(12): 3439 - 3447.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. G. Anthony, S. Khan, J. Costa, M. S. Pais, and L. Bogre
The Arabidopsis Protein Kinase PTI1-2 Is Activated by Convergent Phosphatidic Acid and Oxidative Stress Signaling Pathways Downstream of PDK1 and OXI1
J. Biol. Chem., December 8, 2006; 281(49): 37536 - 37546.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. M. Chilley, S. A. Casson, P. Tarkowski, N. Hawkins, K. L.-C. Wang, P. J. Hussey, M. Beale, J. R. Ecker, G. K. Sandberg, and K. Lindsey
The POLARIS Peptide of Arabidopsis Regulates Auxin Transport and Root Growth via Effects on Ethylene Signaling
PLANT CELL, November 1, 2006; 18(11): 3058 - 3072.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. Bartolini and G. G. Gundersen
Generation of noncentrosomal microtubule arrays
J. Cell Sci., October 15, 2006; 119(20): 4155 - 4163.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Shoji, K. Suzuki, T. Abe, Y. Kaneko, H. Shi, J.-K. Zhu, A. Rus, P. M. Hasegawa, and T. Hashimoto
Salt Stress Affects Cortical Microtubule Organization and Helical Growth in Arabidopsis
Plant Cell Physiol., August 1, 2006; 47(8): 1158 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Hirase, T. Hamada, T. J. Itoh, T. Shimmen, and S. Sonobe
n-Butanol induces depolymerization of microtubules in vivo and in vitro
Plant Cell Physiol., July 1, 2006; 47(7): 1004 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. Cai, E. Ovidi, S. Romagnoli, M. Vantard, M. Cresti, and A. Tiezzi
Identification and Characterization of Plasma Membrane Proteins that Bind to Microtubules in Pollen Tubes and Generative Cells of Tobacco
Plant Cell Physiol., April 1, 2005; 46(4): 563 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology, December 1, 2004; 136(4): 3884 - 3891.
[Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
Encounters between Dynamic Cortical Microtubules Promote Ordering of the Cortical Array through Angle-Dependent Modifications of Microtubule Behavior
PLANT CELL, December 1, 2004; 16(12): 3274 - 3284.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. D. X. Chuong, A. G. Good, G. J. Taylor, M. C. Freeman, G. B. G. Moorhead, and D. G. Muench
Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells
Mol. Cell. Proteomics, October 1, 2004; 3(10): 970 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
The Cortical Microtubule Array: From Dynamics to Organization
PLANT CELL, October 1, 2004; 16(10): 2546 - 2552.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-L. Zheng, Y. Gui, G. Du, M. A. Frohman, and D.-Q. Peng
Calphostin-C Induction of Vascular Smooth Muscle Cell Apoptosis Proceeds through Phospholipase D and Microtubule Inhibition
J. Biol. Chem., February 20, 2004; 279(8): 7112 - 7118.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Dhonukshe, A. M. Laxalt, J. Goedhart, T. W. J. Gadella, and T. Munnik
Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells
PLANT CELL, November 1, 2003; 15(11): 2666 - 2679.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Baluska, J. Samaj, P. Wojtaszek, D. Volkmann, and D. Menzel
Cytoskeleton-Plasma Membrane-Cell Wall Continuum in Plants. Emerging Links Revisited
Plant Physiology, October 1, 2003; 133(2): 482 - 491.
[Full Text] [PDF]

Home page
 Plant CellHome page
W. Zhang, C. Wang, C. Qin, T. Wood, G. Olafsdottir, R. Welti, and X. Wang
The Oleate-Stimulated Phospholipase D, PLD{delta}, and Phosphatidic Acid Decrease H2O2-Induced Cell Death in Arabidopsis
PLANT CELL, October 1, 2003; 15(10): 2285 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. O. Wasteneys
Microtubules Show their Sensitive Nature
Plant Cell Physiol., July 15, 2003; 44(7): 653 - 654.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Sivaguru, S. Pike, W. Gassmann, and T. I. Baskin
Aluminum Rapidly Depolymerizes Cortical Microtubules and Depolarizes the Plasma Membrane: Evidence that these Responses are Mediated by a Glutamate Receptor
Plant Cell Physiol., July 15, 2003; 44(7): 667 - 675.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Gardiner, D. A. Collings, J. D. I. Harper, and J. Marc
The Effects of the Phospholipase D-Antagonist 1-Butanol on Seedling Development and Microtubule Organisation in Arabidopsis
Plant Cell Physiol., July 15, 2003; 44(7): 687 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Drykova, V. Cenklova, V. Sulimenko, J. Volc, P. Draber, and P. Binarova
Plant {gamma}-Tubulin Interacts with {alpha}{beta}-Tubulin Dimers and Forms Membrane-Associated Complexes
PLANT CELL, February 1, 2003; 15(2): 465 - 480.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Qin, C. Wang, and X. Wang
Kinetic Analysis of Arabidopsis Phospholipase Ddelta . SUBSTRATE PREFERENCE AND MECHANISM OF ACTIVATION BY Ca2+ AND PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE
J. Biol. Chem., December 13, 2002; 277(51): 49685 - 49690.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Zhong, D. H. Burk, W. H. Morrison III, and Z.-H. Ye
A Kinesin-Like Protein Is Essential for Oriented Deposition of Cellulose Microfibrils and Cell Wall Strength
PLANT CELL, December 1, 2002; 14(12): 3101 - 3117.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Hallouin, T. Ghelis, M. Brault, F. Bardat, D. Cornel, E. Miginiac, J.-P. Rona, B. Sotta, and E. Jeannette
Plasmalemma Abscisic Acid Perception Leads to RAB18 Expression via Phospholipase D Activation in Arabidopsis Suspension Cells
Plant Physiology, September 1, 2002; 130(1): 265 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Qin and X. Wang
The Arabidopsis Phospholipase D Family. Characterization of a Calcium-Independent and Phosphatidylcholine-Selective PLDzeta 1 with Distinct Regulatory Domains
Plant Physiology, March 1, 2002; 128(3): 1057 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. D. X. Chuong, R. T. Mullen, and D. G. Muench
Identification of a Rice RNA- and Microtubule-binding Protein as the Multifunctional Protein, a Peroxisomal Enzyme Involved in the beta -Oxidation of Fatty Acids
J. Biol. Chem., January 18, 2002; 277(4): 2419 - 2429.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists