First published online January 29, 2002; 10.1105/tpc.010278
The Plant Cell, Vol. 14, 165-179,
January 2002, Copyright © 2002,
American Society of Plant Biologists
Mutation of a Chitinase-Like Gene Causes Ectopic Deposition of Lignin, Aberrant Cell Shapes, and Overproduction of Ethylene
Ruiqin Zhonga,
Stanley J. Kaysb,
Betty P. Schroederb and
Zheng-Hua Ye1,a
a Department of Botany, University of Georgia, Athens, Georgia 30602
b Department of Horticulture, University of Georgia, Athens, Georgia 30602
1 To whom correspondence should be addressed. E-mail ye{at}dogwood.botany.uga.edu; fax 706-542-1832
Chitinase-like proteins have long been proposed to play roles in normal plant growth and development, but no mutations in chitinase-like genes have been obtained previously to support this hypothesis. In this study, we have shown that the gene responsible for the elp1 mutation in Arabidopsis encodes a chitinase-like protein (AtCTL1). Mutation of this chitinase-like gene caused ectopic deposition of lignin and aberrant shapes of cells with incomplete cell walls in the pith of inflorescence stems. The AtCTL1 gene was expressed in all organs during normal plant growth and development, but it was not induced by wounding, salicylic acid, pectin fragments, or ethylene. Consistent with its ubiquitous expression pattern, mutation of the AtCTL1 gene affected many aspects of plant growth and development, including exaggerated hook curvature, reduced length and increased diameter of hypocotyls in dark-grown seedlings, and reduced root length and increased number of root hairs in light-grown seedlings. The mutant phenotypes could be rescued partially by ethylene inhibitors, and ethylene production in the mutant was significantly greater than in the wild type. Together, these results suggest that AtCTL1, a chitinase-like gene, is essential for normal plant growth and development in Arabidopsis.
This article has been cited by other articles:
|
|
|
|
 
X. Sun, C. Hu, Q. Tan, J. Liu, and H. Liu
Effects of molybdenum on expression of cold-responsive genes in abscisic acid (ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress
Ann. Bot.,
June 1, 2009;
(2009)
mcp133v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hayashi, T. Ishii, T. Matsunaga, R. Tominaga, T. Kuromori, T. Wada, K. Shinozaki, and T. Hirayama
The Glycerophosphoryl Diester Phosphodiesterase-Like Proteins SHV3 and its Homologs Play Important Roles in Cell Wall Organization
Plant Cell Physiol.,
October 1, 2008;
49(10):
1522 - 1535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhang, K. Ohyama, J. Boudet, Z. Chen, J. Yang, M. Zhang, T. Muranaka, C. Maurel, J.-K. Zhu, and Z. Gong
Dolichol Biosynthesis and Its Effects on the Unfolded Protein Response and Abiotic Stress Resistance in Arabidopsis
PLANT CELL,
July 1, 2008;
20(7):
1879 - 1898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Wang, J. Li, and M. Yuan
Salt Tolerance Requires Cortical Microtubule Reorganization in Arabidopsis
Plant Cell Physiol.,
November 1, 2007;
48(11):
1534 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Minic, E. Jamet, L. Negroni, P Arsene der Garabedian, M. Zivy, and L. Jouanin
A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases
J. Exp. Bot.,
July 1, 2007;
58(10):
2503 - 2512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Ko, J. H. Kim, S. S. Jayanty, G. A. Howe, and K.-H. Han
Loss of function of COBRA, a determinant of oriented cell expansion, invokes cellular defence responses in Arabidopsis thaliana
J. Exp. Bot.,
September 1, 2006;
57(12):
2923 - 2936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Knogge and D. Scheel
LysM receptors recognize friend and foe
PNAS,
July 18, 2006;
103(29):
10829 - 10830.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Geisler-Lee, M. Geisler, P. M. Coutinho, B. Segerman, N. Nishikubo, J. Takahashi, H. Aspeborg, S. Djerbi, E. Master, S. Andersson-Gunneras, et al.
Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses
Plant Physiology,
March 1, 2006;
140(3):
946 - 962.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Abdulrazzak, B. Pollet, J. Ehlting, K. Larsen, C. Asnaghi, S. Ronseau, C. Proux, M. Erhardt, V. Seltzer, J.-P. Renou, et al.
A coumaroyl-ester-3-hydroxylase Insertion Mutant Reveals the Existence of Nonredundant meta-Hydroxylation Pathways and Essential Roles for Phenolic Precursors in Cell Expansion and Plant Growth
Plant Physiology,
January 1, 2006;
140(1):
30 - 48.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Wheeler, B. Smith-White, V. Chetvernin, S. Resenchuk, S. M. Dombrowski, S. W. Pechous, T. Tatusova, and J. Ostell
Plant Genome Resources at the National Center for Biotechnology Information
Plant Physiology,
July 1, 2005;
138(3):
1280 - 1288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Persson, H. Wei, J. Milne, G. P. Page, and C. R. Somerville
Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets
PNAS,
June 14, 2005;
102(24):
8633 - 8638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Rogers, C. Dubos, I. F. Cullis, C. Surman, M. Poole, J. Willment, S. D. Mansfield, and M. M. Campbell
Light, the circadian clock, and sugar perception in the control of lignin biosynthesis
J. Exp. Bot.,
June 1, 2005;
56(416):
1651 - 1663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Aspeborg, J. Schrader, P. M. Coutinho, M. Stam, A. Kallas, S. Djerbi, P. Nilsson, S. Denman, B. Amini, F. Sterky, et al.
Carbohydrate-Active Enzymes Involved in the Secondary Cell Wall Biogenesis in Hybrid Aspen
Plant Physiology,
March 1, 2005;
137(3):
983 - 997.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Somerville, S. Bauer, G. Brininstool, M. Facette, T. Hamann, J. Milne, E. Osborne, A. Paredez, S. Persson, T. Raab, et al.
Toward a Systems Approach to Understanding Plant Cell Walls
Science,
December 24, 2004;
306(5705):
2206 - 2211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yang, S. Chaudhuri, L. Yang, Y. Chen, and B. W. Poovaiah
Calcium/Calmodulin Up-regulates a Cytoplasmic Receptor-like Kinase in Plants
J. Biol. Chem.,
October 8, 2004;
279(41):
42552 - 42559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Yokoyama and K. Nishitani
Genomic Basis for Cell-Wall Diversity in Plants. A Comparative Approach to Gene Families in Rice and Arabidopsis
Plant Cell Physiol.,
September 15, 2004;
45(9):
1111 - 1121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nagae, A. Nozawa, N. Koizumi, H. Sano, H. Hashimoto, M. Sato, and T. Shimizu
The Crystal Structure of the Novel Calcium-binding Protein AtCBL2 from Arabidopsis thaliana
J. Biol. Chem.,
October 24, 2003;
278(43):
42240 - 42246.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Hong, U. Lee, and E. Vierling
Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures
Plant Physiology,
June 1, 2003;
132(2):
757 - 767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. H. Burk and Z.-H. Ye
Alteration of Oriented Deposition of Cellulose Microfibrils by Mutation of a Katanin-Like Microtubule-Severing Protein
PLANT CELL,
September 1, 2002;
14(9):
2145 - 2160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ellis, I. Karafyllidis, C. Wasternack, and J. G. Turner
The Arabidopsis Mutant cev1 Links Cell Wall Signaling to Jasmonate and Ethylene Responses
PLANT CELL,
July 1, 2002;
14(7):
1557 - 1566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
