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First published online September 26, 2002; 10.1105/tpc.004259

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The Plant Cell, Vol. 14, 2577-2590, October 2002, Copyright © 2002,
American Society of Plant Biologists

QUASIMODO1 Encodes a Putative Membrane-Bound Glycosyltransferase Required for Normal Pectin Synthesis and Cell Adhesion in Arabidopsis

Sophie Boutona, Edouard Leboeufb, Gregory Mouillec, Marie-Thérèse Leydeckera, Joël Talboteca, Fabienne Granierd, Marc Lahayeb, Herman Höftec and Hoai-Nam Truong1,a

a Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, 78026 Versailles Cedex, France
b Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Institut National de la Recherche Agronomique Centre de Nantes, Rue de la Géraudière, BP 71627, 44316 Nantes Cedex, France
c Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, 78026 Versailles Cedex, France
d Génétique et Amélioration des Plantes, Institut National de la Recherche Agronomique Centre de Versailles-Grignon, 78026 Versailles Cedex, France

1 To whom correspondence should be addressed. E-mail truong{at}versailles.inra.fr; fax 33-1-30-83-30-96

Pectins are a highly complex family of cell wall polysaccharides. As a result of a lack of specific mutants, it has been difficult to study the biosynthesis of pectins and their role in vivo. We have isolated two allelic mutants, named quasimodo1 (qua1-1 and qua1-2), that are dwarfed and show reduced cell adhesion. Mutant cell walls showed a 25% reduction in galacturonic acid levels compared with the wild type, indicating reduced pectin content, whereas neutral sugars remained unchanged. Immersion immunofluorescence with the JIM5 and JIM7 monoclonal antibodies that recognize homogalacturonan epitopes revealed less labeling of mutant roots compared with the wild type. Both mutants carry a T-DNA insertion in a gene (QUA1) that encodes a putative membrane-bound glycosyltransferase of family 8. We present evidence for the possible involvement of a glycosyltransferase of this family in the synthesis of pectic polysaccharides, suggesting that other members of this large multigene family in Arabidopsis also may be important for pectin biosynthesis. The mutant phenotype is consistent with a central role for pectins in cell adhesion.




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