Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online September 16, 2002; 10.1105/tpc.002998

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
14/10/2613    most recent
tpc.002998v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Snowman, B. N.
Right arrow Articles by Staiger, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Snowman, B. N.
Right arrow Articles by Staiger, C. J.
Agricola
Right arrow Articles by Snowman, B. N.
Right arrow Articles by Staiger, C. J.
The Plant Cell, Vol. 14, 2613-2626, October 2002, Copyright © 2002,
American Society of Plant Biologists

Signal-Mediated Depolymerization of Actin in Pollen during the Self-Incompatibility Response

Benjamin N. Snowmana, David R. Kovarb, Galina Shevchenkoa, Vernonica E. Franklin-Tong1,a and Christopher J. Staigerb

a School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
b Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392

1 To whom correspondence should be addressed. E-mail v.e.franklin-tong{at}bham.ac.uk; fax 44-121-414-5925

Signal perception and the integration of signals into networks that effect cellular changes is essential for all cells. The self-incompatibility (SI) response in field poppy pollen triggers a Ca2+-dependent signaling cascade that results in the inhibition of incompatible pollen. SI also stimulates dramatic alterations in the actin cytoskeleton. By measuring the amount of filamentous (F-) actin in pollen before and during the SI response, we demonstrate that SI stimulates a rapid and large reduction in F-actin level that is sustained for at least 1 h. This represents quantitative evidence for stimulus-mediated depolymerization of F-actin in plant cells by a defined biological stimulus. Surprisingly, there are remarkably few examples of sustained reductions in F-actin levels stimulated by a biologically relevant ligand. Actin depolymerization also was achieved in pollen by treatments that increase cytosolic free Ca2+ artificially, providing evidence that actin is a target for the Ca2+ signals triggered by the SI response. By determining the cellular concentrations and binding constants for native profilin from poppy pollen, we show that profilin has Ca2+-dependent monomeric actin-sequestering activity. Although profilin is likely to contribute to stimulus-mediated actin depolymerization, our data suggest a role for additional actin binding proteins. We propose that Ca2+-mediated depolymerization of F-actin may be a mechanism whereby SI-induced tip growth inhibition is achieved.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L. Cardenas, A. Lovy-Wheeler, J. G. Kunkel, and P. K. Hepler
Pollen Tube Growth Oscillations and Intracellular Calcium Levels Are Reversibly Modulated by Actin Polymerization
Plant Physiology, April 1, 2008; 146(4): 1611 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. S. Poulter, S. Vatovec, and V. E. Franklin-Tong
Microtubules Are a Target for Self-Incompatibility Signaling in Papaver Pollen
Plant Physiology, March 1, 2008; 146(3): 1358 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Bosch and V. E. Franklin-Tong
Self-incompatibility in Papaver: signalling to trigger PCD in incompatible pollen
J. Exp. Bot., February 1, 2008; 59(3): 481 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Jones, N. Sharopova, D. P. Lohar, J. Q. Zhang, K. A. VandenBosch, and G. C. Walker
Differential response of the plant Medicago truncatula to its symbiont Sinorhizobium meliloti or an exopolysaccharide-deficient mutant
PNAS, January 15, 2008; 105(2): 704 - 709.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Bosch and V. E. Franklin-Tong
Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen
PNAS, November 13, 2007; 104(46): 18327 - 18332.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. K. Kandasamy, B. Burgos-Rivera, E. C. McKinney, D. R. Ruzicka, and R. B. Meagher
Class-Specific Interaction of Profilin and ADF Isovariants with Actin in the Regulation of Plant Development
PLANT CELL, October 1, 2007; 19(10): 3111 - 3126.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Li, J. Samaj, and V. E. Franklin-Tong
A Mitogen-Activated Protein Kinase Signals to Programmed Cell Death Induced by Self-Incompatibility in Papaver Pollen
Plant Physiology, September 1, 2007; 145(1): 236 - 245.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Chaudhry, C. Guerin, M. von Witsch, L. Blanchoin, and C. J. Staiger
Identification of Arabidopsis Cyclase-associated Protein 1 as the First Nucleotide Exchange Factor for Plant Actin
Mol. Biol. Cell, August 1, 2007; 18(8): 3002 - 3014.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Iwano, H. Shiba, K. Matoba, T. Miwa, M. Funato, T. Entani, P. Nakayama, H. Shimosato, A. Takaoka, A. Isogai, et al.
Actin Dynamics in Papilla Cells of Brassica rapa during Self- and Cross-Pollination
Plant Physiology, May 1, 2007; 144(1): 72 - 81.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
B. McClure
Pollen-Pistil Signaling in Self-Incompatible Poppy: Does It Allow More Efficient Resource Allocation in the Pistil?
Sci. Signal., April 24, 2007; 2007(383): pe17 - pe17.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. G. Thomas, S. Huang, S. Li, C. J. Staiger, and V. E. Franklin-Tong
Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen
J. Cell Biol., July 17, 2006; 174(2): 221 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Huang, L. Gao, L. Blanchoin, and C. J. Staiger
Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid
Mol. Biol. Cell, April 1, 2006; 17(4): 1946 - 1958.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Yokota, M. Tominaga, I. Mabuchi, Y. Tsuji, C. J. Staiger, K. Oiwa, and T. Shimmen
Plant Villin, Lily P-135-ABP, Possesses G-Actin Binding Activity and Accelerates the Polymerization and Depolymerization of Actin in a Ca2+-Sensitive Manner
Plant Cell Physiol., October 1, 2005; 46(10): 1690 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Michelot, C. Guerin, S. Huang, M. Ingouff, S. Richard, N. Rodiuc, C. J. Staiger, and L. Blanchoin
The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1
PLANT CELL, August 1, 2005; 17(8): 2296 - 2313.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Z.-l. Shang, L.-g. Ma, H.-l. Zhang, R.-r. He, X.-c. Wang, S.-j. Cui, and D.-y. Sun
Ca2+ Influx into Lily Pollen Grains Through a Hyperpolarization-activated Ca2+-permeable Channel Which Can be Regulated by Extracellular CaM
Plant Cell Physiol., April 1, 2005; 46(4): 598 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Takemoto and A. R. Hardham
The Cytoskeleton as a Regulator and Target of Biotic Interactions in Plants
Plant Physiology, December 1, 2004; 136(4): 3864 - 3876.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology, December 1, 2004; 136(4): 3884 - 3891.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Fan, J. Hou, X. Chen, F. Chaudhry, C. J. Staiger, and H. Ren
Identification and Characterization of a Ca2+-Dependent Actin Filament-Severing Protein from Lily Pollen
Plant Physiology, December 1, 2004; 136(4): 3979 - 3989.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Huang, L. Blanchoin, F. Chaudhry, V. E. Franklin-Tong, and C. J. Staiger
A Gelsolin-like Protein from Papaver rhoeas Pollen (PrABP80) Stimulates Calcium-regulated Severing and Depolymerization of Actin Filaments
J. Biol. Chem., May 28, 2004; 279(22): 23364 - 23375.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Geitmann, W. McConnaughey, I. Lang-Pauluzzi, V. E. Franklin-Tong, and A. M. C. Emons
Cytomechanical Properties of Papaver Pollen Tubes Are Altered after Self-Incompatibility Challenge
Biophys. J., May 1, 2004; 86(5): 3314 - 3323.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Huang, L. Blanchoin, D. R. Kovar, and C. J. Staiger
Arabidopsis Capping Protein (AtCP) Is a Heterodimer That Regulates Assembly at the Barbed Ends of Actin Filaments
J. Biol. Chem., November 7, 2003; 278(45): 44832 - 44842.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. J. Staiger and V. E. Franklin-Tong
The actin cytoskeleton is a target of the self-incompatibility response in Papaver rhoeas
J. Exp. Bot., January 1, 2003; 54(380): 103 - 113.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. J. Rudd and V. E. Franklin-Tong
Signals and targets of the self-incompatibility response in pollen of Papaver rhoeas
J. Exp. Bot., January 1, 2003; 54(380): 141 - 148.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2002 by the American Society of Plant Biologists