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Plasma Membrane–Associated ROP10 Small GTPase Is a Specific Negative Regulator of Abscisic Acid Responses in Arabidopsis

^a Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, California 92521
^b Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla, California 92093
^c Department of Biology, Indiana University–Purdue University at Indianapolis, Indianapolis, Indiana 46202

² To whom correspondence should be addressed. E-mail zhenbiao.yang{at}ucr.edu; fax 909-787-4437

Abscisic acid (ABA) is an important plant hormone that modulates seed germination and plant growth and stress responses, but its signaling remains poorly understood. We investigated the role of ROP10, a member of the Arabidopsis Rop subfamily of Rho GTPases, in ABA signaling. A null rop10 mutant exhibits enhanced responses to ABA in seed germination, root elongation, and stomatal closure assays and in the induction of expression of the transcription factor MYB2, but it shows wild-type levels of ABA and normal responses to other hormones. Consistently, transgenic expression of a constitutively active form of ROP10 reduces ABA inhibition of seed germination, whereas dominant-negative mutants of ROP10 enhance ABA response and partially suppress abi2. Furthermore, ABA specifically downregulates ROP10 transcription in root tips. ROP10 is localized to the plasma membrane (PM), and PM localization is crucial for its function. These results suggest that ROP10 is a PM-localized signaling molecule that is involved specifically in the negative regulation of ABA signaling.

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