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A Two-Component Mn²⁺-Sensing System Negatively Regulates Expression of the mntCAB Operon in Synechocystis

^a Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
^b Department of Molecular Biomechanics, School of Life Science, Graduate University of Advanced Studies, Okazaki 444-8585, Japan
^c Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia
^d Department of Genetics, Moscow State University, Moscow 119899, Russia
^e Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan

¹ To whom correspondence should be addressed. E-mail murata{at}nibb.ac.jp; fax 81-564-54-4866

Mn is an essential component of the oxygen-evolving machinery of photosynthesis and is an essential cofactor of several important enzymes, such as Mn–superoxide dismutase and Mn-catalase. The availability of Mn in the environment varies, and little is known about the mechanisms for maintaining cytoplasmic Mn²⁺ ion homeostasis. Using a DNA microarray, we screened knockout libraries of His kinases and response regulators of Synechocystis sp PCC 6803 to identify possible participants in this process. We identified a His kinase, ManS, which might sense the extracellular concentration of Mn²⁺ ions, and a response regulator, ManR, which might regulate the expression of the mntCAB operon for the ABC-type transporter of Mn²⁺ ions. Furthermore, analysis with the DNA microarray and by reverse transcription PCR suggested that ManS produces a signal that activates ManR, which represses the expression of the mntCAB operon. At low concentrations of Mn²⁺ ions, ManS does not generate a signal, with resulting inactivation of ManR and subsequent expression of the mntCAB operon.

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