First published online October 11, 2002; 10.1105/tpc.004341
The Plant Cell, Vol. 14, 2957-2974,
November 2002, Copyright © 2002,
American Society of Plant Biologists
Proteomic Characterization of the Small Subunit of Chlamydomonas reinhardtii Chloroplast Ribosome
Identification of a Novel S1 Domain–Containing Protein and Unusually Large Orthologs of Bacterial S2, S3, and S5
Kenichi Yamaguchia,
Susana Prieto1,a,
María Verónica Belignia,
Paul A. Haynesb,
W. Hayes McDonaldc,
John R. Yates, IIIc and
Stephen P. Mayfield2,a
a Department of Cell Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037
b Torrey Mesa Research Institute of Syngenta, San Diego, California 92121
c Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037
2 To whom correspondence should be addressed. E-mail mayfield{at}scripps.edu; fax 858-784-9840
To understand how chloroplast mRNAs are translated into functional proteins, a detailed understanding of all of the components of chloroplast translation is needed. To this end, we performed a proteomic analysis of the plastid ribosomal proteins in the small subunit of the chloroplast ribosome from the green alga Chlamydomonas reinhardtii. Twenty proteins were identified, including orthologs of Escherichia coli S1, S2, S3, S4, S5, S6, S7, S9, S10, S12, S13, S14, S15, S16, S17, S18, S19, S20, and S21 and a homolog of spinach plastid-specific ribosomal protein-3 (PSRP-3). In addition, a novel S1 domain–containing protein, PSRP-7, was identified. Among the identified proteins, S2 (57 kD), S3 (76 kD), and S5 (84 kD) are prominently larger than their E. coli or spinach counterparts, containing N-terminal extensions (S2 and S5) or insertion sequence (S3). Structural predictions based on the crystal structure of the bacterial 30S subunit suggest that the additional domains of S2, S3, and S5 are located adjacent to each other on the solvent side near the binding site of the S1 protein. These additional domains may interact with the S1 protein and PSRP-7 to function in aspects of mRNA recognition and translation initiation that are unique to the Chlamydomonas chloroplast.
This article has been cited by other articles:
|
|
|
|
 
J. Uniacke and W. Zerges
Photosystem II Assembly and Repair Are Differentially Localized in Chlamydomonas
PLANT CELL,
November 1, 2007;
19(11):
3640 - 3654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mutsuda and M. Sugiura
Translation Initiation of Cyanobacterial rbcS mRNAs Requires the 38-kDa Ribosomal Protein S1 but Not the Shine-Dalgarno Sequence: DEVELOPMENT OF A CYANOBACTERIAL IN VITRO TRANSLATION SYSTEM
J. Biol. Chem.,
December 15, 2006;
281(50):
38314 - 38321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmidt, G. Gessner, M. Luff, I. Heiland, V. Wagner, M. Kaminski, S. Geimer, N. Eitzinger, T. Reissenweber, O. Voytsekh, et al.
Proteomic Analysis of the Eyespot of Chlamydomonas reinhardtii Provides Novel Insights into Its Components and Tactic Movements
PLANT CELL,
August 1, 2006;
18(8):
1908 - 1930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Wagner, G. Gessner, I. Heiland, M. Kaminski, S. Hawat, K. Scheffler, and M. Mittag
Analysis of the Phosphoproteome of Chlamydomonas reinhardtii Provides New Insights into Various Cellular Pathways
Eukaryot. Cell,
March 1, 2006;
5(3):
457 - 468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Naumann, E. J. Stauber, A. Busch, F. Sommer, and M. Hippler
N-terminal Processing of Lhca3 Is a Key Step in Remodeling of the Photosystem I-Light-harvesting Complex Under Iron Deficiency in Chlamydomonas reinhardtii
J. Biol. Chem.,
May 27, 2005;
280(21):
20431 - 20441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-F. Chang, K. Szick-Miranda, S. Pan, and J. Bailey-Serres
Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes
Plant Physiology,
March 1, 2005;
137(3):
848 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. V. Beligni, K. Yamaguchi, and S. P. Mayfield
Chloroplast Elongation Factor Ts Pro-Protein Is an Evolutionarily Conserved Fusion with the S1 Domain-Containing Plastid-Specific Ribosomal Protein-7
PLANT CELL,
December 1, 2004;
16(12):
3357 - 3369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hirose and M. Sugiura
Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence
Nucleic Acids Res.,
June 30, 2004;
32(11):
3503 - 3510.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Morita-Yamamuro, T. Tsutsui, A. Tanaka, and J. Yamaguchi
Knock-out of the Plastid Ribosomal Protein S21 Causes Impaired Photosynthesis and Sugar-Response during Germination and Seedling Development in Arabidopsis thaliana
Plant Cell Physiol.,
June 15, 2004;
45(6):
781 - 788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Baginsky and W. Gruissem
Chloroplast proteomics: potentials and challenges
J. Exp. Bot.,
June 1, 2004;
55(400):
1213 - 1220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Lonosky, X. Zhang, V. G. Honavar, D. L. Dobbs, A. Fu, and S. R. Rodermel
A Proteomic Analysis of Maize Chloroplast Biogenesis
Plant Physiology,
February 1, 2004;
134(2):
560 - 574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamaguchi, M. V. Beligni, S. Prieto, P. A. Haynes, W. H. McDonald, J. R. Yates III, and S. P. Mayfield
Proteomic Characterization of the Chlamydomonas reinhardtii Chloroplast Ribosome: IDENTIFICATION OF PROTEINS UNIQUE TO THE 70 S RIBOSOME
J. Biol. Chem.,
September 5, 2003;
278(36):
33774 - 33785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Maul, J. W. Lilly, L. Cui, C. W. dePamphilis, W. Miller, E. H. Harris, and D. B. Stern
The Chlamydomonas reinhardtii Plastid Chromosome: Islands of Genes in a Sea of Repeats
PLANT CELL,
November 1, 2002;
14(11):
2659 - 2679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
