First published online November 20, 2002; 10.1105/tpc.006809
The Plant Cell, Vol. 14, 3255-3269,
December 2002, Copyright © 2002,
American Society of Plant Biologists
A Peptide Chain Release Factor 2 Affects the Stability of UGA-Containing Transcripts in Arabidopsis Chloroplasts
Jörg Meurer1,2,a,
Lina Lezhneva1,a,
Katrin Amanna,
Manfred Gödela,
Staver Bezhanib,
Irena Sherametib and
Ralf Oelmüllerb
a Ludwig-Maximilians-Universität München, Department I, Botanik, Menzingerstrasse 67, 80638 München, Germany
b Friedrich-Schiller-Universität, Institut für Allgemeine Botanik, Dornburger Strasse 159, 07743 Jena, Germany
2 To whom correspondence should be addressed. E-mail meurer{at}botanik.biologie.uni-muenchen.de; fax 49-89-1782274
Positional cloning of the hcf109 (high chlorophyll fluorescence) mutation in Arabidopsis has identified a nucleus-encoded, plastid-localized release factor 2–like protein, AtprfB, indicating that the processes of translational termination in chloroplasts resemble those of eubacteria. Control of atprfB expression by light and tissues is connected to chloroplast development. A point mutation at the last nucleotide of the second intron causes a new splice site farther downstream, resulting in a deletion of seven amino acid residues in the N-terminal region of the Hcf109 protein. The mutation causes decreased stability of UGA-containing mRNAs. Our data suggest that transcripts with UGA stop codons are terminated exclusively by AtprfB in chloroplasts and that AtprfB is involved in the regulation of both mRNA stability and protein synthesis. Furthermore, sequence data reveal a +1 frameshift at an internal in-frame TGA stop codon in the progenitor prfB gene of cyanobacteria. The expression pattern and functions of atprfB could reflect evolutionary driving forces toward the conservation of TGA stop codons exclusively in plastid genomes of land plants.
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