Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moseley, J. L.
Right arrow Articles by Merchant, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moseley, J. L.
Right arrow Articles by Merchant, S.
Agricola
Right arrow Articles by Moseley, J. L.
Right arrow Articles by Merchant, S.
The Plant Cell, Vol. 14, 673-688, March 2002, Copyright © 2002,
American Society of Plant Biologists

Reciprocal Expression of Two Candidate Di-Iron Enzymes Affecting Photosystem I and Light-Harvesting Complex Accumulation

Jeffrey L. Moseleya,b, M. Dudley Pagea, Nancy P. Alderc, Mats Eriksson1,a, Jeanette Quinn2,a, Feiris Sotoa, Steven M. Thegc, Michael Hipplerd and Sabeeha Merchant3,a,b

a Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569
b Molecular Biology Institute, University of California, Los Angeles, California 90095-1569
c Section of Plant Biology, University of California, Davis, California 95616
d Plant Physiology, Friedrich-Schiller-Universität, Dornburger St. 159, 07743 Jena, Germany

3 To whom correspondence should be addressed. E-mail merchant{at}chem.ucla.edu; fax 310-206-1035

Crd1 (Copper response defect 1), which is required for the maintenance of photosystem I and its associated light-harvesting complexes in copper-deficient (-Cu) and oxygen-deficient (-O2) Chlamydomonas reinhardtii cells, is localized to the thylakoid membrane. A related protein, Cth1 (Copper target homolog 1), is shown to have a similar but not identical function by genetic suppressor analysis of gain-of-function sct1 (suppressor of copper target 1) strains that are transposon-containing alleles at CTH1. The pattern of Crd1 versus Cth1 accumulation is reciprocal; Crd1 abundance is increased in -Cu or -O2 cells, whereas Cth1 accumulates in copper-sufficient (+Cu), oxygenated cells. This expression pattern is determined by a single trans-acting regulatory locus, CRR1 (COPPER RESPONSE REGULATOR 1), which activates transcription in -Cu cells. In +Cu cells, a 2.1-kb Cth1 mRNA is produced and translated, whereas Crd1 is transcribed only at basal levels, leading to Cth1 accumulation in +Cu cells. In -Cu cells, CRR1 function determines the activation of Crd1 expression and the production of an alternative 3.1-kb Cth1 mRNA that is extended at the 5' end relative to the 2.1-kb mRNA. Synthesis of the 3.1-kb mRNA, which encodes six small upstream open reading frames that possibly result in poor translation, blocks the downstream promoter through transcriptional occlusion. Fluorescence analysis of wild-type, crd1, and sct1 strains indicates that copper-responsive adjustment of the Cth1:Crd1 ratio results in modification of the interactions between photosystem I and associated light-harvesting complexes. The tightly coordinated CRR1-dependent regulation of isoenzymes Cth1 and Crd1 reinforces the notion that copper plays a specific role in the maintenance of chlorophyll proteins.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. M. Terauchi, S.-F. Lu, M. Zaffagnini, S. Tappa, M. Hirasawa, J. N. Tripathy, D. B. Knaff, P. J. Farmer, S. D. Lemaire, T. Hase, et al.
Pattern of Expression and Substrate Specificity of Chloroplast Ferredoxins from Chlamydomonas reinhardtii
J. Biol. Chem., September 18, 2009; 284(38): 25867 - 25878.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K.-H. Tang, J. Wen, X. Li, and R. E. Blankenship
Role of the AcsF Protein in Chloroflexus aurantiacus
J. Bacteriol., June 1, 2009; 191(11): 3580 - 3587.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. D. Page, J. Kropat, P. P. Hamel, and S. S. Merchant
Two Chlamydomonas CTR Copper Transporters with a Novel Cys-Met Motif Are Localized to the Plasma Membrane and Function in Copper Assimilation
PLANT CELL, March 1, 2009; 21(3): 928 - 943.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. Majeran, B. Zybailov, A. J. Ytterberg, J. Dunsmore, Q. Sun, and K. J. van Wijk
Consequences of C4 Differentiation for Chloroplast Membrane Proteomes in Maize Mesophyll and Bundle Sheath Cells
Mol. Cell. Proteomics, September 1, 2008; 7(9): 1609 - 1638.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
W. Y. Bang, I. S. Jeong, D. W. Kim, C. H. Im, C. Ji, S. M. Hwang, S. W. Kim, Y. S. Son, J. Jeong, T. Shiina, et al.
Role of Arabidopsis CHL27 Protein for Photosynthesis, Chloroplast Development and Gene Expression Profiling
Plant Cell Physiol., September 1, 2008; 49(9): 1350 - 1363.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Minamizaki, T. Mizoguchi, T. Goto, H. Tamiaki, and Y. Fujita
Identification of Two Homologous Genes, chlAI and chlAII, That Are Differentially Involved in Isocyclic Ring Formation of Chlorophyll a in the Cyanobacterium Synechocystis sp. PCC 6803
J. Biol. Chem., February 1, 2008; 283(5): 2684 - 2692.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. T. Croft, M. Moulin, M. E. Webb, and A. G. Smith
Thiamine biosynthesis in algae is regulated by riboswitches
PNAS, December 26, 2007; 104(52): 20770 - 20775.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K.-S. Kim, S. Kustu, and W. Inwood
Natural History of Transposition in the Green Alga Chlamydomonas reinhardtii: Use of the AMT4 Locus as an Experimental System
Genetics, August 1, 2006; 173(4): 2005 - 2019.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Varsano, S. G. Wolf, and U. Pick
A Chlorophyll a/b-binding Protein Homolog That Is Induced by Iron Deficiency Is Associated with Enlarged Photosystem I Units in the Eucaryotic Alga Dunaliella salina
J. Biol. Chem., April 14, 2006; 281(15): 10305 - 10315.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Kropat, S. Tottey, R. P. Birkenbihl, N. Depege, P. Huijser, and S. Merchant
A regulator of nutritional copper signaling in Chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element
PNAS, December 20, 2005; 102(51): 18730 - 18735.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. A. Martens, P.-Y. J. Wu, and F. Winston
Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae
Genes & Dev., November 15, 2005; 19(22): 2695 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Lohr, C.-S. Im, and A. R. Grossman
Genome-Based Examination of Chlorophyll and Carotenoid Biosynthesis in Chlamydomonas reinhardtii
Plant Physiology, May 1, 2005; 138(1): 490 - 515.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Rzeznicka, C. J. Walker, T. Westergren, C. G. Kannangara, D. von Wettstein, S. Merchant, S. P. Gough, and M. Hansson
Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX monomethyl ester cyclase involved in chlorophyll biosynthesis
PNAS, April 19, 2005; 102(16): 5886 - 5891.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Hanikenne, U. Kramer, V. Demoulin, and D. Baurain
A Comparative Inventory of Metal Transporters in the Green Alga Chlamydomonas reinhardtii and the Red Alga Cyanidioschizon merolae
Plant Physiology, February 1, 2005; 137(2): 428 - 446.
[Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. Nield, K. Redding, and M. Hippler
Remodeling of Light-Harvesting Protein Complexes in Chlamydomonas in Response to Environmental Changes
Eukaryot. Cell, December 1, 2004; 3(6): 1370 - 1380.
[Full Text] [PDF]

Home page
 Plant CellHome page
K. Amann, L. Lezhneva, G. Wanner, R. G. Herrmann, and J. Meurer
ACCUMULATION OF PHOTOSYSTEM ONE1, a Member of a Novel Gene Family, Is Required for Accumulation of [4Fe-4S] Cluster-Containing Chloroplast Complexes and Antenna Proteins
PLANT CELL, November 1, 2004; 16(11): 3084 - 3097.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Z. Zhang, J. Shrager, M. Jain, C.-W. Chang, O. Vallon, and A. R. Grossman
Insights into the Survival of Chlamydomonas reinhardtii during Sulfur Starvation Based on Microarray Analysis of Gene Expression
Eukaryot. Cell, October 1, 2004; 3(5): 1331 - 1348.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Eriksson, J. L. Moseley, S. Tottey, J. A. del Campo, J. Quinn, Y. Kim, and S. Merchant
Genetic Dissection of Nutritional Copper Signaling in Chlamydomonas Distinguishes Regulatory and Target Genes
Genetics, October 1, 2004; 168(2): 795 - 807.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Matsumoto, T. Obayashi, Y. Sasaki-Sekimoto, H. Ohta, K.-i. Takamiya, and T. Masuda
Gene Expression Profiling of the Tetrapyrrole Metabolic Pathway in Arabidopsis with a Mini-Array System
Plant Physiology, August 1, 2004; 135(4): 2379 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. C. Rutherford and A. J. Bird
Metal-Responsive Transcription Factors That Regulate Iron, Zinc, and Copper Homeostasis in Eukaryotic Cells
Eukaryot. Cell, February 1, 2004; 3(1): 1 - 13.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Tottey, M. A. Block, M. Allen, T. Westergren, C. Albrieux, H. V. Scheller, S. Merchant, and P. E. Jensen
Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide
PNAS, December 23, 2003; 100(26): 16119 - 16124.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. M. Quinn, J. Kropat, and S. Merchant
Copper Response Element and Crr1-Dependent Ni2+-Responsive Promoter for Induced, Reversible Gene Expression in Chlamydomonas reinhardtii
Eukaryot. Cell, October 1, 2003; 2(5): 995 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Evilevitch, L. Lavelle, C. M. Knobler, E. Raspaud, and W. M. Gelbart
Osmotic pressure inhibition of DNA ejection from phage
PNAS, August 5, 2003; 100(16): 9292 - 9295.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2002 by the American Society of Plant Biologists