First published online May 2, 2002; 10.1105/tpc.001271
The Plant Cell, Vol. 14, 1173-1183,
May 2002, Copyright © 2002,
American Society of Plant Biologists
Extensive Interallelic Polymorphisms Drive Meiotic Recombination into a Crossover Pathway
Hugo K. Dooner1,a,b
a Waksman Institute, Rutgers University, Piscataway, New Jersey 08855
b Department of Plant Sciences, Rutgers University, New Brunswick, New Jersey 08901
1 To whom correspondence should be addressed. E-mail dooner{at}waksman.rutgers.edu; fax 732-445-5735
Recombinants isolated from most meiotic intragenic recombination experiments in maize, but not in yeast, are borne principally on crossover chromosomes. This excess of crossovers is not explained readily by the canonical double-strand break repair model of recombination, proposed to account for a large body of yeast data, which predicts that crossovers (COs) and noncrossovers (NCOs) should be recovered equally. An attempt has been made here to identify general rules governing the recovery of the CO and NCO classes of intragenic recombinants in maize. Recombination was analyzed in bz heterozygotes between a variety of mutations derived from the same or different progenitor alleles. The mutations include point mutations, transposon insertions, and transposon excision footprints. Consequently, the differences between the bz heteroalleles ranged from just two nucleotides to many nucleotides, indels, and insertions. In this article, allelic pairs differing at only two positions are referred to as dimorphic to distinguish them from polymorphic pairs, which differ at multiple positions. The present study has revealed the following effects at these bz heteroalleles: (1) recombination between polymorphic heteroalleles produces mostly CO chromosomes; (2) recombination between dimorphic heteroalleles produces both CO and NCO chromosomes, in ratios apparently dependent on the nature of the heteroalleles; and (3) in dimorphic heterozygotes, the two NCO classes are recovered in approximately equal numbers when the two mutations are point mutations but not when one or both mutations are insertions. These observations are discussed in light of a recent version of the double-strand break repair model of recombination that postulates separate pathways for the formation of CO and NCO products.
This article has been cited by other articles:
|
|
|
|
 
H. K. Dooner and L. He
Maize Genome Structure Variation: Interplay between Retrotransposon Polymorphisms and Genic Recombination
PLANT CELL,
February 1, 2008;
20(2):
249 - 258.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Yandeau-Nelson, B. J. Nikolau, and P. S. Schnable
Effects of trans-acting Genetic Modifiers on Meiotic Recombination Across the a1-sh2 Interval of Maize
Genetics,
September 1, 2006;
174(1):
101 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Yandeau-Nelson, Y. Xia, J. Li, M. G. Neuffer, and P. S. Schnable
Unequal Sister Chromatid and Homolog Recombination at a Tandem Duplication of the a1 Locus in Maize
Genetics,
August 1, 2006;
173(4):
2211 - 2226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yao and P. S. Schnable
Cis-effects on Meiotic Recombination Across Distinct a1-sh2 Intervals in a Common Zea Genetic Background
Genetics,
August 1, 2005;
170(4):
1929 - 1944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Yandeau-Nelson, Q. Zhou, H. Yao, X. Xu, B. J. Nikolau, and P. S. Schnable
MuDR Transposase Increases the Frequency of Meiotic Crossovers in the Vicinity of a Mu Insertion in the Maize a1 Gene
Genetics,
February 1, 2005;
169(2):
917 - 929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Palaisa, M. Morgante, S. Tingey, and A. Rafalski
Long-range patterns of diversity and linkage disequilibrium surrounding the maize Y1 gene are indicative of an asymmetric selective sweep
PNAS,
June 29, 2004;
101(26):
9885 - 9890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
