This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (33) Citing Articles via Google Scholar Google Scholar Articles by Yanovsky, M. J. Articles by Casal, J. J. Search for Related Content PubMed PubMed Citation Articles by Yanovsky, M. J. Articles by Casal, J. J. Agricola Articles by Yanovsky, M. J. Articles by Casal, J. J.

Missense Mutation in the PAS2 Domain of Phytochrome A Impairs Subnuclear Localization and a Subset of Responses

^a IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, Av San Martín 4453, 1417-Buenos Aires, Argentina
^b Institut für Biologie II, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
^c Biology Department, M.V. Lomonosov Moscow State University, Moscow, Russia
^d Institute of Plant Biology, Biological Research Center, P.O. Box 521, H-6701, Szeged, Hungary
^e Instituto de Investigaciones Bioquímicas Fundación Campomar, Av Patricias Argentinas 435, 1405-Buenos Aires, Argentina

⁴ To whom correspondence should be addressed. E-mail casal{at}ifeva.edu.ar; fax 54-11-45-14-87-30

Phytochrome A signaling shows two photobiologically discrete outputs: so-called very-low-fluence responses (VLFR) and high-irradiance responses (HIR). By modifying previous screening protocols, we isolated two Arabidopsis mutants retaining VLFR and lacking HIR. Phytochrome A negatively or positively regulates phytochrome B signaling, depending on light conditions. These mutants retained the negative but lacked the positive regulation. Both mutants carry the novel phyA-302 allele, in which Glu-777 (a residue conserved in angiosperm phytochromes) changed to Lys in the PAS2 motif of the C-terminal domain. The phyA-302 mutants showed a 50% reduction in phytochrome A levels in darkness, but this difference was compensated for by greater stability under continuous far-red light. phyA-302:green fluorescent protein fusion proteins showed normal translocation from the cytosol to the nucleus under continuous far-red light but failed to produce nuclear spots, suggesting that nuclear speckles could be involved in HIR signaling and phytochrome A degradation. We propose that the PAS2 domain of phytochrome A is necessary to initiate signaling in HIR but not in VLFR, likely via interaction with a specific partner.

This article has been cited by other articles:

				E. Kevei, E. Schafer, and F. Nagy Light-regulated nucleo-cytoplasmic partitioning of phytochromes J. Exp. Bot., September 27, 2007; (2007) erm145v1. [Abstract] [Full Text] [PDF]

				K. A. Oliverio, M. Crepy, E. L. Martin-Tryon, R. Milich, S. L. Harmer, J. Putterill, M. J. Yanovsky, and J. J. Casal GIGANTEA Regulates Phytochrome A-Mediated Photomorphogenesis Independently of Its Role in the Circadian Clock Plant Physiology, May 1, 2007; 144(1): 495 - 502. [Abstract] [Full Text] [PDF]

				J. L. Mateos, J. P. Luppi, O. B. Ogorodnikova, V. A. Sineshchekov, M. J. Yanovsky, S. E. Braslavsky, W. Gartner, and J. J. Casal Functional and Biochemical Analysis of the N-terminal Domain of Phytochrome A J. Biol. Chem., November 10, 2006; 281(45): 34421 - 34429. [Abstract] [Full Text] [PDF]

				V. Tillemans, I. Leponce, G. Rausin, L. Dispa, and P. Motte Insights into Nuclear Organization in Plants as Revealed by the Dynamic Distribution of Arabidopsis SR Splicing Factors PLANT CELL, November 1, 2006; 18(11): 3218 - 3234. [Abstract] [Full Text] [PDF]

				J. L. Heazlewood, J. Tonti-Filippini, R. E. Verboom, and A. H. Millar Combining Experimental and Predicted Datasets for Determination of the Subcellular Location of Proteins in Arabidopsis Plant Physiology, October 1, 2005; 139(2): 598 - 609. [Abstract] [Full Text] [PDF]

				T. M. A. Magliano, J. F. Botto, A. V. Godoy, V. V. Symonds, A. M. Lloyd, and J. J. Casal New Arabidopsis Recombinant Inbred Lines (Landsberg erecta x Nossen) Reveal Natural Variation in Phytochrome-Mediated Responses Plant Physiology, June 1, 2005; 138(2): 1126 - 1135. [Abstract] [Full Text] [PDF]

				D. Bauer, A. Viczian, S. Kircher, T. Nobis, R. Nitschke, T. Kunkel, K. C.S. Panigrahi, E. Adam, E. Fejes, E. Schafer, et al. Constitutive Photomorphogenesis 1 and Multiple Photoreceptors Control Degradation of Phytochrome Interacting Factor 3, a Transcription Factor Required for Light Signaling in Arabidopsis PLANT CELL, June 1, 2004; 16(6): 1433 - 1445. [Abstract] [Full Text] [PDF]

				C. K.-Y. Ng, T. Kinoshita, S. Pandey, K.-i. Shimazaki, and S. M. Assmann Abscisic Acid Induces Rapid Subnuclear Reorganization in Guard Cells Plant Physiology, April 1, 2004; 134(4): 1327 - 1331. [Full Text] [PDF]

				C. Kami, K. Mukougawa, T. Muramoto, A. Yokota, T. Shinomura, J. C. Lagarias, and T. Kohchi Complementation of phytochrome chromophore-deficient Arabidopsis by expression of phycocyanobilin:ferredoxin oxidoreductase PNAS, January 27, 2004; 101(4): 1099 - 1104. [Abstract] [Full Text] [PDF]

				J. F. Botto, C. Alonso-Blanco, I. Garzaron, R. A. Sanchez, and J. J. Casal The Cape Verde Islands Allele of Cryptochrome 2 Enhances Cotyledon Unfolding in the Absence of Blue Light in Arabidopsis Plant Physiology, December 1, 2003; 133(4): 1547 - 1556. [Abstract] [Full Text]

				M. Chen, R. Schwab, and J. Chory Characterization of the requirements for localization of phytochrome B to nuclear bodies PNAS, November 25, 2003; 100(24): 14493 - 14498. [Abstract] [Full Text] [PDF]

				S. Mathews, J. G. Burleigh, and M. J. Donoghue Adaptive Evolution in the Photosensory Domain of Phytochrome A in Early Angiosperms Mol. Biol. Evol., July 1, 2003; 20(7): 1087 - 1097. [Abstract] [Full Text] [PDF]