Endogenous and Silencing-Associated Small RNAs in Plants

doi:10.1105/tpc.003210

Endogenous and Silencing-Associated Small RNAs in Plants

Cesar Llave, Kristin D. Kasschau, Maggie A. Rector and James C. Carrington¹

Center for Gene Research and Biotechnology, and Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331

¹ To whom correspondence should be addressed. E-mail carrington{at}orst.edu; fax 541-737-3045

A large set of endogenous small RNAs of predominantly 21 to24 nucleotides was identified in Arabidopsis. These small RNAsresembled micro-RNAs from animals and were similar in size tosmall interfering RNAs that accumulated during RNA silencingtriggered by multiple types of inducers. Among the 125 sequencesidentified, the vast majority (90%) arose from intergenic regions,although small RNAs corresponding to predicted protein-codinggenes, transposon-like sequences, and a structural RNA genealso were identified. Evidence consistent with the derivationof small RNAs of both polarities, and from highly base-pairedprecursors, was obtained through the identification and analysisof clusters of small RNA loci. The accumulation of specificsmall RNAs was regulated developmentally. We propose that Arabidopsissmall RNAs participate in a wide range of post-transcriptionaland epigenetic events.

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T. A. Volpe, C. Kidner, I. M. Hall, G. Teng, S. I. S. Grewal, and R. A. Martienssen
Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi
Science, September 13, 2002; 297(5588): 1833 - 1837.
[Abstract] [Full Text] [PDF]

R. A. Jorgensen
RNA traffics information systemically in plants
PNAS, September 3, 2002; 99(18): 11561 - 11563.
[Full Text] [PDF]