First published online July 18, 2002; 10.1105/tpc.002576
The Plant Cell, Vol. 14, 1869-1883,
August 2002, Copyright © 2002,
American Society of Plant Biologists
Plant Colonization by the Vascular Wilt Fungus Fusarium oxysporum Requires FOW1, a Gene Encoding a Mitochondrial Protein
Iori Inouea,
Fumio Namikib and
Takashi Tsuge1,a
a Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
b National Agricultural Research Center for Kyushu-Okinawa Region, Nishigoshi-Machi, Kikuchi-Gun 861-1192, Japan
1 To whom correspondence should be addressed. E-mail ttsuge{at}agr.nagoya-u.ac.jp; fax 81-52-789-4012
The soil-borne fungus Fusarium oxysporum causes vascular wilts of a wide variety of plant species by directly penetrating roots and colonizing the vascular tissue. The pathogenicity mutant B60 of the melon wilt pathogen F. oxysporum f. sp. melonis was isolated previously by restriction enzyme–mediated DNA integration mutagenesis. Molecular analysis of B60 identified the affected gene, designated FOW1, which encodes a protein with strong similarity to mitochondrial carrier proteins of yeast. Although the FOW1 insertional mutant and gene-targeted mutants showed normal growth and conidiation in culture, they showed markedly reduced virulence as a result of a defect in the ability to colonize the plant tissue. Mitochondrial import of Fow1 was verified using strains expressing the Fow1–green fluorescent protein fusion proteins. The FOW1-targeted mutants of the tomato wilt pathogen F. oxysporum f. sp. lycopersici also showed reduced virulence. These data strongly suggest that FOW1 encodes a mitochondrial carrier protein that is required specifically for colonization in the plant tissue by F. oxysporum.
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