First published online December 13, 2002; 10.1105/tpc.007096
The Plant Cell, Vol. 15, 271-284,
January 2003, Copyright © 2003,
American Society of Plant Biologists
The Transcription Factors HvABI5 and HvVP1 Are Required for the Abscisic Acid Induction of Gene Expression in Barley Aleurone Cells
Jose Casaretto and
Tuan-hua David Ho1
Plant Biology Program, Department of Biology, Washington University, St. Louis, Missouri 63130
1 To whom correspondence should be addressed. E-mail ho{at}biology.wustl.edu; fax 314-935-4432
The abscisic acid (ABA) response promoter complexes (ABRCs) of the HVA1 and HVA22 genes have been shown to confer ABA-induced gene expression in cereals. A barley basic domain/Leu zipper (bZIP) transcription factor, HvABI5, is able to recognize ABRCs in vitro in a sequence-specific manner and to transactivate ABRC– -glucuronidase reporter genes when introduced to barley aleurone cells via particle bombardment. This transactivation is dependent on the presence of another transcription factor, HvVP1, and cannot be blocked by the negative regulator abi1-1. Using the double-stranded RNA interference technique, we show that HvABI5 and HvVP1 are necessary for the ABA induction of gene expression but have no effect on another hormone-regulated process, the gibberellin-induced and ABA-suppressed expression of  -amylase. Our work indicates that although other typical plant bZIP transcription factors may bind ABRCs in vitro, HvABI5 is related to a subfamily of bZIPs responsible for the ABA induction of gene expression. Furthermore, HvABI5 and HvVP1 are not involved in the ABA suppression of gene expression.
This article has been cited by other articles:
|
|
|
|
 
K. Nakashima, Y. Ito, and K. Yamaguchi-Shinozaki
Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses
Plant Physiology,
January 1, 2009;
149(1):
88 - 95.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-J. Guo and T.-H. David Ho
An Abscisic Acid-Induced Protein, HVA22, Inhibits Gibberellin-Mediated Programmed Cell Death in Cereal Aleurone Cells
Plant Physiology,
August 1, 2008;
147(4):
1710 - 1722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Cao, L. M. Costa, C. Biderre-Petit, B. Kbhaya, N. Dey, P. Perez, D. R. McCarty, J. F. Gutierrez-Marcos, and P. W. Becraft
Abscisic Acid and Stress Signals Induce Viviparous1 Expression in Seed and Vegetative Tissues of Maize
Plant Physiology,
February 1, 2007;
143(2):
720 - 731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Furihata, K. Maruyama, Y. Fujita, T. Umezawa, R. Yoshida, K. Shinozaki, and K. Yamaguchi-Shinozaki
Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1
PNAS,
February 7, 2006;
103(6):
1988 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-i. Choi, H.-J. Park, J. H. Park, S. Kim, M.-Y. Im, H.-H. Seo, Y.-W. Kim, I. Hwang, and S. Y. Kim
Arabidopsis Calcium-Dependent Protein Kinase AtCPK32 Interacts with ABF4, a Transcriptional Regulator of Abscisic Acid-Responsive Gene Expression, and Modulates Its Activity
Plant Physiology,
December 1, 2005;
139(4):
1750 - 1761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Suzuki, M. G. Ketterling, and D. R. McCarty
Quantitative Statistical Analysis of cis-Regulatory Sequences in ABA/VP1- and CBF/DREB1-Regulated Genes of Arabidopsis
Plant Physiology,
September 1, 2005;
139(1):
437 - 447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Xie, Z.-L. Zhang, X. Zou, J. Huang, P. Ruas, D. Thompson, and Q. J. Shen
Annotations and Functional Analyses of the Rice WRKY Gene Superfamily Reveal Positive and Negative Regulators of Abscisic Acid Signaling in Aleurone Cells
Plant Physiology,
January 1, 2005;
137(1):
176 - 189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zou, J. R. Seemann, D. Neuman, and Q. J. Shen
A WRKY Gene from Creosote Bush Encodes an Activator of the Abscisic Acid Signaling Pathway
J. Biol. Chem.,
December 31, 2004;
279(53):
55770 - 55779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
