Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online October 23, 2003; 10.1105/tpc.015842

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/11/2636    most recent
tpc.015842v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (45)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.
Right arrow Articles by Li, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.
Right arrow Articles by Li, X.
Agricola
Right arrow Articles by Zhang, Y.
Right arrow Articles by Li, X.
The Plant Cell, Vol. 15, 2636-2646, November 2003, www.plantcell.org ©2003, American Society of Plant Biologists

A Gain-of-Function Mutation in a Plant Disease Resistance Gene Leads to Constitutive Activation of Downstream Signal Transduction Pathways in suppressor of npr1-1, constitutive 1

Yuelin Zhanga, Sandra Goritschniga, Xinnian Dongb and Xin Li1,a

a Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
b Developmental, Cell, and Molecular Biology Group, Biology Department, Duke University, Durham, North Carolina 27708

1 To whom correspondence should be addressed. E-mail xinli{at}interchange.ubc.ca; fax 604-822-6089

Plants have evolved sophisticated defense mechanisms against pathogen infections, during which resistance (R) genes play central roles in recognizing pathogens and initiating defense cascades. Most of the cloned R genes share two common domains: the central domain, which encodes a nucleotide binding adaptor shared by APAF-1, certain R proteins, and CED-4 (NB-ARC), plus a C-terminal region that encodes Leu-rich repeats (LRR). In Arabidopsis, a dominant mutant, suppressor of npr1-1, constitutive 1 (snc1), was identified previously that constitutively expresses pathogenesis-related (PR) genes and resistance against both Pseudomonas syringae pv maculicola ES4326 and Peronospora parasitica Noco2. The snc1 mutation was mapped to the RPP4 cluster. In snc1, one of the TIR-NB-LRR–type R genes contains a point mutation that results in a single amino acid change from Glu to Lys in the region between NB-ARC and LRR. Deletions of this R gene in snc1 reverted the plants to wild-type morphology and completely abolished constitutive PR gene expression and disease resistance. The constitutive activation of the defense responses was not the result of the overexpression of the R gene, because its expression level was not altered in snc1. Our data suggest that the point mutation in snc1 renders the R gene constitutively active without interaction with pathogens. To analyze signal transduction pathways downstream of snc1, epistasis analyses between snc1 and pad4-1 or eds5-3 were performed. Although the resistance signaling in snc1 was fully dependent on PAD4, it was only partially affected by blocking salicylic acid (SA) synthesis, suggesting that snc1 activates both SA-dependent and SA-independent resistance pathways.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Goritschnig, T. Weihmann, Y. Zhang, P. Fobert, P. McCourt, and X. Li
A Novel Role for Protein Farnesylation in Plant Innate Immunity
Plant Physiology, September 1, 2008; 148(1): 348 - 357.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
D.-L. Yang, Q. Li, Y.-W. Deng, Y.-G. Lou, M.-Y. Wang, G.-X. Zhou, Y.-Y. Zhang, and Z.-H. He
Altered Disease Development in the eui Mutants and Eui Overexpressors Indicates that Gibberellins Negatively Regulate Rice Basal Disease Resistance
Mol Plant, May 13, 2008; (2008) ssn021v2.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
J. Liu and G. Coaker
Nuclear Trafficking During Plant Innate Immunity
Mol Plant, May 1, 2008; 1(3): 411 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
Z. Zhang, A. Lenk, M. X. Andersson, T. Gjetting, C. Pedersen, M. E. Nielsen, M.-A. Newman, B.-H. Hou, S. C. Somerville, and H. Thordal-Christensen
A Lesion-Mimic Syntaxin Double Mutant in Arabidopsis Reveals Novel Complexity of Pathogen Defense Signaling
Mol Plant, May 1, 2008; 1(3): 510 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Positive and Negative Feedback Coordinate Regulation of Disease Resistance Gene Expression
PLANT CELL, September 1, 2007; 19(9): 2700 - 2702.
[Full Text] [PDF]

Home page
 Plant CellHome page
H. Yi and E. J. Richards
A Cluster of Disease Resistance Genes in Arabidopsis Is Coordinately Regulated by Transcriptional Activation and RNA Silencing
PLANT CELL, September 1, 2007; 19(9): 2929 - 2939.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Yang, S. Yang, Y. Li, and J. Hua
The Arabidopsis BAP1 and BAP2 Genes Are General Inhibitors of Programmed Cell Death
Plant Physiology, September 1, 2007; 145(1): 135 - 146.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Palma, Q. Zhao, Y. T. Cheng, D. Bi, J. Monaghan, W. Cheng, Y. Zhang, and X. Li
Regulation of plant innate immunity by three proteins in a complex conserved across the plant and animal kingdoms
Genes & Dev., June 15, 2007; 21(12): 1484 - 1493.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
A. M. Missaoui, D. V. Phillips, and H. R. Boerma
DNA Marker Analysis of 'Davis' Soybean and Its Descendants for the Rcs3 Gene Conferring Resistance to Cercospora sojina
Crop Sci., May 31, 2007; 47(3): 1263 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. I.L. Tameling and D. C. Baulcombe
Physical Association of the NB-LRR Resistance Protein Rx with a Ran GTPase-Activating Protein Is Required for Extreme Resistance to Potato virus X
PLANT CELL, May 1, 2007; 19(5): 1682 - 1694.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Y. Yoo, K. Miura, J. B. Jin, J. Lee, H. C. Park, D. E. Salt, D.-J. Yun, R. A. Bressan, and P. M. Hasegawa
SIZ1 Small Ubiquitin-Like Modifier E3 Ligase Facilitates Basal Thermotolerance in Arabidopsis Independent of Salicylic Acid
Plant Physiology, December 1, 2006; 142(4): 1548 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. A. Thompson and F. L. Goggin
Transcriptomics and functional genomics of plant defence induction by phloem-feeding insects
J. Exp. Bot., March 1, 2006; 57(4): 755 - 766.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Pegadaraju, C. Knepper, J. Reese, and J. Shah
Premature Leaf Senescence Modulated by the Arabidopsis PHYTOALEXIN DEFICIENT4 Gene Is Associated with Defense against the Phloem-Feeding Green Peach Aphid
Plant Physiology, December 1, 2005; 139(4): 1927 - 1934.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. J. Feys, M. Wiermer, R. A. Bhat, L. J. Moisan, N. Medina-Escobar, C. Neu, A. Cabral, and J. E. Parker
Arabidopsis SENESCENCE-ASSOCIATED GENE101 Stabilizes and Signals within an ENHANCED DISEASE SUSCEPTIBILITY1 Complex in Plant Innate Immunity
PLANT CELL, September 1, 2005; 17(9): 2601 - 2613.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Nakayama, J. M. Arroyo, J. Simorowski, B. May, R. Martienssen, and V. F. Irish
Gene Trap Lines Define Domains of Gene Regulation in Arabidopsis Petals and Stamens
PLANT CELL, September 1, 2005; 17(9): 2486 - 2506.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Zhang and X. Li
A Putative Nucleoporin 96 Is Required for Both Basal Defense and Constitutive Resistance Responses Mediated by suppressor of npr1-1,constitutive 1
PLANT CELL, April 1, 2005; 17(4): 1306 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. J. Heidel, J. D. Clarke, J. Antonovics, and X. Dong
Fitness Costs of Mutations Affecting the Systemic Acquired Resistance Pathway in Arabidopsis thaliana
Genetics, December 1, 2004; 168(4): 2197 - 2206.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Belkhadir, Z. Nimchuk, D. A. Hubert, D. Mackey, and J. L. Dangl
Arabidopsis RIN4 Negatively Regulates Disease Resistance Mediated by RPS2 and RPM1 Downstream or Independent of the NDR1 Signal Modulator and Is Not Required for the Virulence Functions of Bacterial Type III Effectors AvrRpt2 or AvrRpm1
PLANT CELL, October 1, 2004; 16(10): 2822 - 2835.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Xiao, L. Dai, F. Liu, Z. Wang, W. Peng, and D. Xie
COS1: An Arabidopsis coronatine insensitive1 Suppressor Essential for Regulation of Jasmonate-Mediated Plant Defense and Senescence
PLANT CELL, May 1, 2004; 16(5): 1132 - 1142.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Yang and J. Hua
A Haplotype-Specific Resistance Gene Regulated by BONZAI1 Mediates Temperature-Dependent Growth Control in Arabidopsis
PLANT CELL, April 1, 2004; 16(4): 1060 - 1071.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists