Plant Cell EPICENTRE Biotechnologies
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online September 24, 2003; 10.1105/tpc.014977

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
15/11/2666    most recent
tpc.014977v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (79)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Munnik, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Munnik, T.
Agricola
Right arrow Articles by Dhonukshe, P.
Right arrow Articles by Munnik, T.
The Plant Cell, Vol. 15, 2666-2679, November 2003, www.plantcell.org ©2003, American Society of Plant Biologists

Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells

Pankaj Dhonukshea, Ana M. Laxalt1,b, Joachim Goedharta, Theodorus W. J. Gadellaa and Teun Munnik2,b

a Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, NL-1090 GB Amsterdam, The Netherlands
b Section of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, NL-1090 GB Amsterdam, The Netherlands

2 To whom correspondence should be addressed. E-mail munnik{at}science.uva.nl; fax 31-20-5257934

A phospholipase D (PLD) was shown recently to decorate microtubules in plant cells. Therefore, we used tobacco BY-2 cells expressing the microtubule reporter GFP-MAP4 to test whether PLD activation affects the organization of plant microtubules. Within 30 min of adding n-butanol, a potent activator of PLD, cortical microtubules were released from the plasma membrane and partially depolymerized, as visualized with four-dimensional confocal imaging. The isomers sec- and tert-butanol, which did not activate PLD, did not affect microtubule organization. The effect of treatment on PLD activation was monitored by the in vivo formation of phosphatidylbutanol, a specific reporter of PLD activity. Tobacco cells also were treated with mastoparan, xylanase, NaCl, and hypoosmotic stress as reported activators of PLD. We confirmed the reports and found that all treatments induced microtubule reorganization and PLD activation within the same time frame. PLD still was activated in microtubule-stabilized (taxol) and microtubule-depolymerized (oryzalin) situations, suggesting that PLD activation triggers microtubular reorganization and not vice versa. Exogenously applied water-soluble synthetic phosphatidic acid did not affect the microtubular cytoskeleton. Cell cycle studies revealed that n-butanol influenced not just interphase cortical microtubules but also those in the preprophase band and phragmoplast, but not those in the spindle structure. Cell growth and division were inhibited in the presence of n-butanol, whereas sec- and tert-butanol had no such effects. Using these novel insights, we propose a model for the mechanism by which PLD activation triggers microtubule reorganization in plant cells.




This article has been cited by other articles:


Home page
 J Exp BotHome page
L. Li, N. Saga, and K. Mikami
Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis
J. Exp. Bot., June 16, 2009; (2009) erp183v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Yamaguchi, M. Kuroda, H. Yamakawa, T. Ashizawa, K. Hirayae, L. Kurimoto, T. Shinya, and N. Shibuya
Suppression of a Phospholipase D Gene, OsPLD{beta}1, Activates Defense Responses and Increases Disease Resistance in Rice
Plant Physiology, May 1, 2009; 150(1): 308 - 319.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
B. O. R. Bargmann, A. M. Laxalt, B. t. Riet, B. van Schooten, E. Merquiol, C. Testerink, M. A. Haring, D. Bartels, and T. Munnik
Multiple PLDs Required for High Salinity and Water Deficit Tolerance in Plants
Plant Cell Physiol., January 1, 2009; 50(1): 78 - 89.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Ambrose and G. O. Wasteneys
CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules
Mol. Biol. Cell, November 1, 2008; 19(11): 4730 - 4737.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Paredez, S. Persson, D. W. Ehrhardt, and C. R. Somerville
Genetic Evidence That Cellulose Synthase Activity Influences Microtubule Cortical Array Organization
Plant Physiology, August 1, 2008; 147(4): 1723 - 1734.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Lanteri, A. M. Laxalt, and L. Lamattina
Nitric Oxide Triggers Phosphatidic Acid Accumulation via Phospholipase D during Auxin-Induced Adventitious Root Formation in Cucumber
Plant Physiology, May 1, 2008; 147(1): 188 - 198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Li, R. Wang, M. Li, L. Li, C. Wang, R. Welti, and X. Wang
Differential Degradation of Extraplastidic and Plastidic Lipids during Freezing and Post-freezing Recovery in Arabidopsis thaliana
J. Biol. Chem., January 4, 2008; 283(1): 461 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. T. Peters, K. O. Logan, A. C. Miller, and D. L. Kropf
Phospholipase D Signaling Regulates Microtubule Organization in the Fucoid Alga Silvetia compressa
Plant Cell Physiol., December 1, 2007; 48(12): 1764 - 1774.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C. Wang, J. Li, and M. Yuan
Salt Tolerance Requires Cortical Microtubule Reorganization in Arabidopsis
Plant Cell Physiol., November 1, 2007; 48(11): 1534 - 1547.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Jiang, B. Yang, N. S. Harris, and M. K. Deyholos
Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots
J. Exp. Bot., October 4, 2007; (2007) erm207v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. D. Teaster, C. M. Motes, Y. Tang, W. C. Wiant, M. Q. Cotter, Y.-S. Wang, A. Kilaru, B. J. Venables, K. H. Hasenstein, G. Gonzalez, et al.
N-Acylethanolamine Metabolism Interacts with Abscisic Acid Signaling in Arabidopsis thaliana Seedlings
PLANT CELL, August 1, 2007; 19(8): 2454 - 2469.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. Li and H.-W. Xue
Arabidopsis PLD{zeta}2 Regulates Vesicle Trafficking and Is Required for Auxin Response
PLANT CELL, January 1, 2007; 19(1): 281 - 295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. G. Anthony, S. Khan, J. Costa, M. S. Pais, and L. Bogre
The Arabidopsis Protein Kinase PTI1-2 Is Activated by Convergent Phosphatidic Acid and Oxidative Stress Signaling Pathways Downstream of PDK1 and OXI1
J. Biol. Chem., December 8, 2006; 281(49): 37536 - 37546.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. Bartolini and G. G. Gundersen
Generation of noncentrosomal microtubule arrays
J. Cell Sci., October 15, 2006; 119(20): 4155 - 4163.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Shoji, K. Suzuki, T. Abe, Y. Kaneko, H. Shi, J.-K. Zhu, A. Rus, P. M. Hasegawa, and T. Hashimoto
Salt Stress Affects Cortical Microtubule Organization and Helical Growth in Arabidopsis
Plant Cell Physiol., August 1, 2006; 47(8): 1158 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Hirase, T. Hamada, T. J. Itoh, T. Shimmen, and S. Sonobe
n-Butanol induces depolymerization of microtubules in vivo and in vitro
Plant Cell Physiol., July 1, 2006; 47(7): 1004 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Wang
Regulatory Functions of Phospholipase D and Phosphatidic Acid in Plant Growth, Development, and Stress Responses
Plant Physiology, October 1, 2005; 139(2): 566 - 573.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology, December 1, 2004; 136(4): 3884 - 3891.
[Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
Encounters between Dynamic Cortical Microtubules Promote Ordering of the Cortical Array through Angle-Dependent Modifications of Microtubule Behavior
PLANT CELL, December 1, 2004; 16(12): 3274 - 3284.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Charron, J.-L. Pingret, M. Chabaud, E.-P. Journet, and D. G. Barker
Pharmacological Evidence That Multiple Phospholipid Signaling Pathways Link Rhizobium Nodulation Factor Perception in Medicago truncatula Root Hairs to Intracellular Responses, Including Ca2+ Spiking and Specific ENOD Gene Expression
Plant Physiology, November 1, 2004; 136(3): 3582 - 3593.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. J. Smith, J. S. Yakisich, G. M. Kapler, E. S. Cole, and D. P. Romero
A {beta}-Tubulin Mutation Selectively Uncouples Nuclear Division and Cytokinesis in Tetrahymena thermophila
Eukaryot. Cell, October 1, 2004; 3(5): 1217 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Dixit and R. Cyr
The Cortical Microtubule Array: From Dynamics to Organization
PLANT CELL, October 1, 2004; 16(10): 2546 - 2552.
[Full Text] [PDF]

Home page
 Plant CellHome page
A. Cruz-Ramirez, J. Lopez-Bucio, G. Ramirez-Pimentel, A. Zurita-Silva, L. Sanchez-Calderon, E. Ramirez-Chavez, E. Gonzalez-Ortega, and L. Herrera-Estrella
The xipotl Mutant of Arabidopsis Reveals a Critical Role for Phospholipid Metabolism in Root System Development and Epidermal Cell Integrity
PLANT CELL, August 1, 2004; 16(8): 2020 - 2034.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists