Regulation of Flowering Time and Floral Organ Identity by a MicroRNA and Its APETALA2-Like Target Genes

doi:10.1105/tpc.016238

Regulation of Flowering Time and Floral Organ Identity by a MicroRNA and Its APETALA2-Like Target Genes

Milo J. Aukerman¹ and Hajime Sakai

E. I duPont de Nemours and Company, Agriculture & Nutrition, Newark, Delaware 19711

^¹ To whom correspondence should be addressed. E-mail milo.j.aukerman{at}usa.dupont.com; fax 302-631-2607

MicroRNAs (miRNAs) are $~$ 21-nucleotide noncoding RNAs that havebeen identified in both animals and plants. Although in animalsthere is direct evidence implicating particular miRNAs in thecontrol of developmental timing, to date it is not known whetherplant miRNAs also play a role in regulating temporal transitions.Through an activation-tagging approach, we demonstrate thatmiRNA 172 (miR172) causes early flowering and disrupts the specificationof floral organ identity when overexpressed in Arabidopsis.miR172 normally is expressed in a temporal manner, consistentwith its proposed role in flowering time control. The regulatorytarget of miR172 is a subfamily of APETALA2 (AP2) transcriptionfactor genes. We present evidence that miR172 downregulatesthese target genes by a translational mechanism rather thanby RNA cleavage. Gain-of-function and loss-of-function analysesindicate that two of the AP2-like target genes normally actas floral repressors, supporting the notion that miR172 regulatesflowering time by downregulating AP2-like target genes.

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[Abstract] [Full Text] [PDF]

G. MEISTER, M. LANDTHALER, Y. DORSETT, and T. TUSCHL
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
RNA, March 1, 2004; 10(3): 544 - 550.
[Abstract] [Full Text] [PDF]