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First published online November 13, 2003; 10.1105/tpc.016428

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The Plant Cell, Vol. 15, 2805-2815, December 2003, www.plantcell.org ©2003, American Society of Plant Biologists

CHLOROPLAST UNUSUAL POSITIONING1 Is Essential for Proper Chloroplast Positioning

Kazusato Oikawaa,b, Masahiro Kasahara1,b, Tomohiro Kiyosue2,b, Takatoshi Kagawa3,b,c, Noriyuki Suetsugua,b, Fumio Takahashia,b, Takeshi Kanegaea, Yasuo Niwad, Akeo Kadotaa and Masamitsu Wada4,a,b

a Department of Biology, Faculty of Science, Graduate School of Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan
b National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan
c Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan
d Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada, Shizuoka 422-8526, Japan

4 To whom correspondence should be addressed. E-mail wada-masamitsu{at}c.metro-u.ac.jp; fax 81-426-77-2559

The intracellular distribution of organelles is a crucial aspect of effective cell function. Chloroplasts change their intracellular positions to optimize photosynthetic activity in response to ambient light conditions. Through screening of mutants of Arabidopsis defective in chloroplast photorelocation movement, we isolated six mutant clones in which chloroplasts gathered at the bottom of the cells and did not distribute throughout cells. These mutants, termed chloroplast unusual positioning (chup), were shown to belong to a single genetic locus by complementation tests. Observation of the positioning of other organelles, such as mitochondria, peroxisomes, and nuclei, revealed that chloroplast positioning and movement are impaired specifically in this mutant, although peroxisomes are distributed along with chloroplasts. The CHUP1 gene encodes a novel protein containing multiple domains, including a coiled-coil domain, an actin binding domain, a Pro-rich region, and two Leu zipper domains. The N-terminal hydrophobic segment of CHUP1 was expressed transiently in leaf cells of Arabidopsis as a fusion protein with the green fluorescent protein. The fusion protein was targeted to envelope membranes of chloroplasts in mesophyll cells, suggesting that CHUP1 may localize in chloroplasts. A glutathione S-transferase fusion protein containing the actin binding domain of CHUP1 was found to bind F-actin in vitro. CHUP1 is a unique gene identified that encodes a protein required for organellar positioning and movement in plant cells.




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