First published online November 13, 2003; 10.1105/tpc.016246
The Plant Cell, Vol. 15, 2826-2842, December 2003,
www.plantcell.org ©2003, American Society of Plant Biologists
A Class-V Myosin Required for Mating, Hyphal Growth, and Pathogenicity in the Dimorphic Plant Pathogen Ustilago maydis
Isabella Weber,
Christian Gruber1 and
Gero Steinberg2
Max-Planck-Institut für Terrestrische Mikrobiologie, D-35043 Marburg, Germany
2 To whom correspondence should be addressed. E-mail gero.steinberg{at}staff.uni-marburg.de; fax 49-6421-178-509
In the early stages of plant infection, yeast-like haploid sporidia of Ustilago maydis respond to pheromone secreted by compatible partners by forming conjugation tubes. These then fuse to generate a dikaryotic hypha that forms appressoria to penetrate the host plant. As a first step toward understanding the structural requirements for these transitions, we have identified myo5, which encodes a class-V myosin. Analysis of conditional and null mutants revealed that Myo5 plays nonessential roles in cytokinesis and morphogenesis in sporidia and is required for hyphal morphology. Consistent with a role in morphogenesis, a functional green fluorescent protein–Myo5 fusion protein localized to the bud tip and the hyphal apex as well as to the septa and the spore wall during later stages of infection. However, the loss of Myo5 did not affect the tip growth of hyphae and sporidia. By contrast, Myo5 was indispensable for conjugation tube formation. Furthermore, myo5 mutants were impaired in the perception of pheromones, which indicates a particular importance of Myo5 in the mating process. Consequently, few mutant hyphae were formed that penetrated the plant epidermis but did not continue invasive growth. These results indicate a crucial role of Myo5 in the morphogenesis, dimorphic switch, and pathogenicity of U. maydis.
This article has been cited by other articles:
|
|
|
|
 
A. Doyle, R. Martin-Garcia, A. T. Coulton, S. Bagley, and D. P. Mulvihill
Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation
J. Cell Sci.,
December 1, 2009;
122(23):
4330 - 4340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sakaguchi, T. Miyaji, G. Tsuji, and Y. Kubo
Kelch Repeat Protein Clakel2p and Calcium Signaling Control Appressorium Development in Colletotrichum lagenarium
Eukaryot. Cell,
January 1, 2008;
7(1):
102 - 111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Riquelme, S. Bartnicki-Garcia, J. M. Gonzalez-Prieto, E. Sanchez-Leon, J. A. Verdin-Ramos, A. Beltran-Aguilar, and M. Freitag
Spitzenkorper Localization and Intracellular Traffic of Green Fluorescent Protein-Labeled CHS-3 and CHS-6 Chitin Synthases in Living Hyphae of Neurospora crassa
Eukaryot. Cell,
October 1, 2007;
6(10):
1853 - 1864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Flor-Parra, S. Castillo-Lluva, and J. Perez-Martin
Polar Growth in the Infectious Hyphae of the Phytopathogen Ustilago maydis Depends on a Virulence-Specific Cyclin
PLANT CELL,
October 1, 2007;
19(10):
3280 - 3296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Castillo-Lluva, I. Alvarez-Tabares, I. Weber, G. Steinberg, and J. Perez-Martin
Sustained cell polarity and virulence in the phytopathogenic fungus Ustilago maydis depends on an essential cyclin-dependent kinase from the Cdk5/Pho85 family
J. Cell Sci.,
May 1, 2007;
120(9):
1584 - 1595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Steinberg
Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper
Eukaryot. Cell,
March 1, 2007;
6(3):
351 - 360.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Becht, J. Konig, and M. Feldbrugge
The RNA-binding protein Rrm4 is essential for polarity in Ustilago maydis and shuttles along microtubules
J. Cell Sci.,
December 1, 2006;
119(23):
4964 - 4973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Flor-Parra, M. Vranes, J. Kamper, and J. Perez-Martin
Biz1, a Zinc Finger Protein Required for Plant Invasion by Ustilago maydis, Regulates the Levels of a Mitotic Cyclin
PLANT CELL,
September 1, 2006;
18(9):
2369 - 2387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Scherer, K. Heimel, V. Starke, and J. Kamper
The Clp1 Protein Is Required for Clamp Formation and Pathogenic Development of Ustilago maydis
PLANT CELL,
September 1, 2006;
18(9):
2388 - 2401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Fuchs, G. Hause, I. Schuchardt, and G. Steinberg
Endocytosis Is Essential for Pathogenic Development in the Corn Smut Fungus Ustilago maydis
PLANT CELL,
August 1, 2006;
18(8):
2066 - 2081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Weber, D. Assmann, E. Thines, and G. Steinberg
Polar Localizing Class V Myosin Chitin Synthases Are Essential during Early Plant Infection in the Plant Pathogenic Fungus Ustilago maydis
PLANT CELL,
January 1, 2006;
18(1):
225 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Castillo-Lluva and J. Perez-Martin
The Induction of the Mating Program in the Phytopathogen Ustilago maydis Is Controlled by a G1 Cyclin
PLANT CELL,
December 1, 2005;
17(12):
3544 - 3560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Schuchardt, D. Assmann, E. Thines, C. Schuberth, and G. Steinberg
Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Hyphal Growth of the Fungus Ustilago maydis
Mol. Biol. Cell,
November 1, 2005;
16(11):
5191 - 5201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Fuchs, I. Manns, and G. Steinberg
Microtubules Are Dispensable for the Initial Pathogenic Development but Required for Long-Distance Hyphal Growth in the Corn Smut Fungus Ustilago maydis
Mol. Biol. Cell,
June 1, 2005;
16(6):
2746 - 2758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bortfeld, K. Auffarth, R. Kahmann, and C. W. Basse
The Ustilago maydis a2 Mating-Type Locus Genes lga2 and rga2 Compromise Pathogenicity in the Absence of the Mitochondrial p32 Family Protein Mrb1
PLANT CELL,
August 1, 2004;
16(8):
2233 - 2248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Adamikova, A. Straube, I. Schulz, and G. Steinberg
Calcium Signaling Is Involved in Dynein-dependent Microtubule Organization
Mol. Biol. Cell,
April 1, 2004;
15(4):
1969 - 1980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
