Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online November 13, 2003; 10.1105/tpc.014712

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/12/2900    most recent
tpc.014712v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (67)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hong, Z.
Right arrow Articles by Matsuoka, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hong, Z.
Right arrow Articles by Matsuoka, M.
Agricola
Right arrow Articles by Hong, Z.
Right arrow Articles by Matsuoka, M.
The Plant Cell, Vol. 15, 2900-2910, December 2003, www.plantcell.org ©2003, American Society of Plant Biologists

A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450

Zhi Honga, Miyako Ueguchi-Tanakaa, Kazuto Umemurab, Sakurako Uozub, Shozo Fujiokac, Suguru Takatsutod, Shigeo Yoshidac, Motoyuki Ashikaria, Hidemi Kitanoa and Makoto Matsuoka1,a

a BioScience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan
b Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
c RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan
d Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan

1 To whom correspondence should be addressed. E-mail makoto{at}nuagr1.agr.nagoya-u.ac.jp; fax 81-52-789-5226

We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of d2 was rescued by exogenous brassinolide treatment. The accumulation profile of BR intermediates in the d2 mutants confirmed that these plants are deficient in late BR biosynthesis. We cloned the D2 gene by map-based cloning. The D2 gene encoded a novel cytochrome P450 classified in CYP90D that is highly similar to the reported BR synthesis enzymes. Introduction of the wild D2 gene into d2-1 rescued the abnormal phenotype of the mutants. In feeding experiments, 3-dehydro-6-deoxoteasterone, 3-dehydroteasterone, and brassinolide effectively caused the lamina joints of the d2 plants to bend, whereas more upstream compounds did not cause bending. Based on these results, we conclude that D2/CYP90D2 catalyzes the steps from 6-deoxoteasterone to 3-dehydro-6-deoxoteasterone and from teasterone to 3-dehydroteasterone in the late BR biosynthesis pathway.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S.-W. Zhang, C.-H. Li, J. Cao, Y.-C. Zhang, S.-Q. Zhang, Y.-F. Xia, D.-Y. Sun, and Y. Sun
Altered Architecture and Enhanced Drought Tolerance in Rice via the Down-Regulation of Indole-3-Acetic Acid by TLD1/OsGH3.13 Activation
Plant Physiology, December 1, 2009; 151(4): 1889 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Tanaka, H. Nakagawa, C. Tomita, Z. Shimatani, M. Ohtake, T. Nomura, C.-J. Jiang, J. G. Dubouzet, S. Kikuchi, H. Sekimoto, et al.
BRASSINOSTEROID UPREGULATED1, Encoding a Helix-Loop-Helix Protein, Is a Novel Gene Involved in Brassinosteroid Signaling and Controls Bending of the Lamina Joint in Rice
Plant Physiology, October 1, 2009; 151(2): 669 - 680.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Nakamura, S. Fujioka, S. Takatsuto, M. Tsujimoto, H. Kitano, S. Yoshida, T. Asami, and T. Nakano
Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in Rice
Plant Cell Physiol., September 1, 2009; 50(9): 1627 - 1635.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
H. Zhang, C. Lian, and Z. Shen
Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa
Ann. Bot., April 1, 2009; 103(6): 923 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Oki, N. Inaba, K. Kitagawa, S. Fujioka, H. Kitano, Y. Fujisawa, H. Kato, and Y. Iwasaki
Function of the {alpha} Subunit of Rice Heterotrimeric G Protein in Brassinosteroid Signaling
Plant Cell Physiol., January 1, 2009; 50(1): 161 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Hirano, K. Aya, T. Hobo, H. Sakakibara, M. Kojima, R. A. Shim, Y. Hasegawa, M. Ueguchi-Tanaka, and M. Matsuoka
Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in Microspore/Pollen and Tapetum of Rice
Plant Cell Physiol., October 1, 2008; 49(10): 1429 - 1450.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
P. Chen, L. Jiang, C. Yu, W. Zhang, J. Wang, and J. Wan
The Identification and Mapping of a Tiller Angle QTL on Rice Chromosome 9
Crop Sci., September 23, 2008; 48(5): 1799 - 1806.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-Y. Bai, L.-Y. Zhang, S. S. Gampala, S.-W. Zhu, W.-Y. Song, K. Chong, and Z.-Y. Wang
Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice
PNAS, August 21, 2007; 104(34): 13839 - 13844.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. J. Dinka, M. A. Campbell, T. Demers, and M. N. Raizada
Predicting the Size of the Progeny Mapping Population Required to Positionally Clone a Gene
Genetics, August 1, 2007; 176(4): 2035 - 2054.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. E. Jager, G. M. Symons, T. Nomura, Y. Yamada, J. J. Smith, S. Yamaguchi, Y. Kamiya, J. L. Weller, T. Yokota, and J. B. Reid
Characterization of Two Brassinosteroid C-6 Oxidase Genes in Pea
Plant Physiology, April 1, 2007; 143(4): 1894 - 1904.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Ohnishi, A.-M. Szatmari, B. Watanabe, S. Fujita, S. Bancos, C. Koncz, M. Lafos, K. Shibata, T. Yokota, K. Sakata, et al.
C-23 Hydroxylation by Arabidopsis CYP90C1 and CYP90D1 Reveals a Novel Shortcut in Brassinosteroid Biosynthesis
PLANT CELL, November 1, 2006; 18(11): 3275 - 3288.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Morinaka, T. Sakamoto, Y. Inukai, M. Agetsuma, H. Kitano, M. Ashikari, and M. Matsuoka
Morphological Alteration Caused by Brassinosteroid Insensitivity Increases the Biomass and Grain Production of Rice
Plant Physiology, July 1, 2006; 141(3): 924 - 931.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Luo, Q. Qian, H. Yin, X. Liu, C. Yin, Y. Lan, J. Tang, Z. Tang, S. Cao, X. Wang, et al.
EUI1, Encoding a Putative Cytochrome P450 Monooxygenase, Regulates Internode Elongation by Modulating Gibberellin Responses in Rice
Plant Cell Physiol., February 1, 2006; 47(2): 181 - 191.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. You, X. Lu, H. Jin, X. Ren, K. Liu, G. Yang, H. Yang, L. Zhu, and G. He
Identification of Quantitative Trait Loci Across Recombinant Inbred Lines and Testcross Populations for Traits of Agronomic Importance in Rice
Genetics, February 1, 2006; 172(2): 1287 - 1300.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Nakamura, S. Fujioka, H. Sunohara, N. Kamiya, Z. Hong, Y. Inukai, K. Miura, S. Takatsuto, S. Yoshida, M. Ueguchi-Tanaka, et al.
The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3, in Rice
Plant Physiology, February 1, 2006; 140(2): 580 - 590.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Hong, M. Ueguchi-Tanaka, S. Fujioka, S. Takatsuto, S. Yoshida, Y. Hasegawa, M. Ashikari, H. Kitano, and M. Matsuoka
The Rice brassinosteroid-deficient dwarf2 Mutant, Defective in the Rice Homolog of Arabidopsis DIMINUTO/DWARF1, Is Rescued by the Endogenously Accumulated Alternative Bioactive Brassinosteroid, Dolichosterone
PLANT CELL, August 1, 2005; 17(8): 2243 - 2254.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T.-W. Kim, J.-Y. Hwang, Y.-S. Kim, S.-H. Joo, S. C. Chang, J. S. Lee, S. Takatsuto, and S.-K. Kim
Arabidopsis CYP85A2, a Cytochrome P450, Mediates the Baeyer-Villiger Oxidation of Castasterone to Brassinolide in Brassinosteroid Biosynthesis
PLANT CELL, August 1, 2005; 17(8): 2397 - 2412.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nomura, T. Kushiro, T. Yokota, Y. Kamiya, G. J. Bishop, and S. Yamaguchi
The Last Reaction Producing Brassinolide Is Catalyzed by Cytochrome P-450s, CYP85A3 in Tomato and CYP85A2 in Arabidopsis
J. Biol. Chem., May 6, 2005; 280(18): 17873 - 17879.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Tanabe, M. Ashikari, S. Fujioka, S. Takatsuto, S. Yoshida, M. Yano, A. Yoshimura, H. Kitano, M. Matsuoka, Y. Fujisawa, et al.
A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length
PLANT CELL, March 1, 2005; 17(3): 776 - 790.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Kurata, K. Miyoshi, K.-I. Nonomura, Y. Yamazaki, and Y. Ito
Rice Mutants and Genes Related to Organ Development, Morphogenesis and Physiological Traits
Plant Cell Physiol., January 15, 2005; 46(1): 48 - 62.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Nomura, C. E. Jager, Y. Kitasaka, K. Takeuchi, M. Fukami, K. Yoneyama, Y. Matsushita, H. Nyunoya, S. Takatsuto, S. Fujioka, et al.
Brassinosteroid Deficiency Due to Truncated Steroid 5{alpha}-Reductase Causes Dwarfism in the lk Mutant of Pea
Plant Physiology, August 1, 2004; 135(4): 2220 - 2229.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T.-W. Kim, S. C. Chang, J. S. Lee, S. Takatsuto, T. Yokota, and S.-K. Kim
Novel Biosynthetic Pathway of Castasterone from Cholesterol in Tomato
Plant Physiology, July 1, 2004; 135(3): 1231 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. R. Nelson, M. A. Schuler, S. M. Paquette, D. Werck-Reichhart, and S. Bak
Comparative Genomics of Rice and Arabidopsis. Analysis of 727 Cytochrome P450 Genes and Pseudogenes from a Monocot and a Dicot
Plant Physiology, June 1, 2004; 135(2): 756 - 772.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists