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Mutations in the Ca²⁺/H⁺ Transporter CAX1 Increase CBF/DREB1 Expression and the Cold-Acclimation Response in Arabidopsis

^a Departamento de Biotecnología, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, 28040 Madrid, Spain
^b Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037

³ To whom correspondence should be addressed. E-mail salinas{at}inia.es; fax 34-91-357-3107

Transient increases in cytosolic free calcium concentration ([Ca²⁺]_cyt) are essential for plant responses to a variety of environmental stimuli, including low temperature. Subsequent reestablishment of [Ca²⁺]_cyt to resting levels by Ca²⁺ pumps and C-REPEAT BINDING FACTOR/DEHYDRATION RESPONSIVE ELEMENT BINDING FACTOR 1 (Ca²⁺/H⁺) antiporters is required for the correct transduction of the signal. We have isolated a cDNA from Arabidopsis that corresponds to a new cold-inducible gene, RARE COLD INDUCIBLE4 (RCI4), which was identical to CALCIUM EXCHANGER 1 (CAX1), a gene that encodes a vacuolar Ca²⁺/H⁺ antiporter involved in the regulation of intracellular Ca²⁺ levels. The expression of CAX1 was induced in response to low temperature through an abscisic acid–independent pathway. To determine the function of CAX1 in Arabidopsis stress tolerance, we identified two T-DNA insertion mutants, cax1-3 and cax1-4, that display reduced tonoplast Ca²⁺/H⁺ antiport activity. The mutants showed no significant differences with respect to the wild type when analyzed for dehydration, high-salt, chilling, or constitutive freezing tolerance. However, they exhibited increased freezing tolerance after cold acclimation, demonstrating that CAX1 plays an important role in this adaptive response. This phenotype correlates with the enhanced expression of CBF/DREB1 genes and their corresponding targets in response to low temperature. Our results indicate that CAX1 ensures the accurate development of the cold-acclimation response in Arabidopsis by controlling the induction of CBF/DREB1 and downstream genes.

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