First published online February 24, 2003; 10.1105/tpc.007922
The Plant Cell, Vol. 15, 626-638,
March 2003, Copyright © 2003,
American Society of Plant Biologists
Disruption Mutations of ADA2b and GCN5 Transcriptional Adaptor Genes Dramatically Affect Arabidopsis Growth, Development, and Gene Expression
Konstantinos E. Vlachonasiosa,
Michael F. Thomashowb,c and
Steven J. Triezenberg1,a
a Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-1319
b Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1219
c Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1325
1 To whom correspondence should be addressed. E-mail triezenb{at}msu.edu; fax 517-353-9334
We previously identified Arabidopsis genes homologous with the yeast ADA2 and GCN5 genes that encode components of the ADA and SAGA histone acetyltransferase complexes. In this report, we explore the biological roles of the Arabidopsis ADA2b and GCN5 genes. T-DNA insertion mutations in ADA2b and GCN5 were found to have pleiotropic effects on plant growth and development, including dwarf size, aberrant root development, and short petals and stamens in flowers. Approximately 5% of the 8200 genes assayed by DNA microarray analysis showed changes of expression in the mutants, three-fourths of which were upregulated and only half of which were altered similarly in the two mutant strains. In cold acclimation experiments, C-repeat binding factors (CBFs) were induced in the mutants as in wild-type plants, but subsequent transcription of cold-regulated (COR) genes was reduced in both mutants. Remarkably, nonacclimated ada2b-1 (but not gcn5-1) mutant plants were more freezing tolerant than nonacclimated wild-type plants, suggesting that ADA2b may directly or indirectly repress a freezing tolerance mechanism that does not require the expression of CBF or COR genes. We conclude that the Arabidopsis ADA2b and GCN5 proteins have both similar and distinct functions in plant growth, development, and gene expression and may be components of both a common coactivator complex and separate complexes with distinct biological activities.
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29(22):
6033 - 6045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Zsindely, T. Pankotai, Z. Ujfaludi, D. Lakatos, O. Komonyi, L. Bodai, L. Tora, and I. M. Boros
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37(20):
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[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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J. Biol. Chem.,
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284(35):
23454 - 23460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Bond, E. S. Dennis, B. J. Pogson, and E. J. Finnegan
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July 1, 2009;
2(4):
724 - 737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kornet and B. Scheres
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PLANT CELL,
April 1, 2009;
21(4):
1070 - 1079.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kornet and B. Scheres
Stem Cell Factors in Plants: Chromatin Connections
Cold Spring Harb Symp Quant Biol,
January 15, 2009;
(2009)
sqb.2008.73.043v1.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.-M. Kim, T. K. To, J. Ishida, T. Morosawa, M. Kawashima, A. Matsui, T. Toyoda, H. Kimura, K. Shinozaki, and M. Seki
Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thaliana
Plant Cell Physiol.,
October 1, 2008;
49(10):
1580 - 1588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wienkoop, K. Morgenthal, F. Wolschin, M. Scholz, J. Selbig, and W. Weckwerth
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Mol. Cell. Proteomics,
September 1, 2008;
7(9):
1725 - 1736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhu, J. C. Jeong, Y. Zhu, I. Sokolchik, S. Miyazaki, J.-K. Zhu, P. M. Hasegawa, H. J. Bohnert, H. Shi, D.-J. Yun, et al.
Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance
PNAS,
March 25, 2008;
105(12):
4945 - 4950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Benhamed, C. Bertrand, C. Servet, and D.-X. Zhou
Arabidopsis GCN5, HD1, and TAF1/HAF2 Interact to Regulate Histone Acetylation Required for Light-Responsive Gene Expression
PLANT CELL,
November 1, 2006;
18(11):
2893 - 2903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Guelman, T. Suganuma, L. Florens, V. Weake, S. K. Swanson, M. P. Washburn, S. M. Abmayr, and J. L. Workman
The Essential Gene wda Encodes a WD40 Repeat Subunit of Drosophila SAGA Required for Histone H3 Acetylation
Mol. Cell. Biol.,
October 1, 2006;
26(19):
7178 - 7189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Bang, S. Kim, A. Ueda, M. Vikram, D. Yun, R. A. Bressan, P. M. Hasegawa, J. Bahk, and H. Koiwa
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Plant Physiology,
October 1, 2006;
142(2):
586 - 594.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Long, C. Ohno, Z. R. Smith, and E. M. Meyerowitz
TOPLESS regulates apical embryonic fate in Arabidopsis.
Science,
June 9, 2006;
312(5779):
1520 - 1523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Guelman, T. Suganuma, L. Florens, S. K. Swanson, C. L. Kiesecker, T. Kusch, S. Anderson, J. R. Yates III, M. P. Washburn, S. M. Abmayr, et al.
Host Cell Factor and an Uncharacterized SANT Domain Protein Are Stable Components of ATAC, a Novel dAda2A/dGcn5-Containing Histone Acetyltransferase Complex in Drosophila
Mol. Cell. Biol.,
February 1, 2006;
26(3):
871 - 882.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Bhatti, M. Livingston, N. Mullapudi, and W. J. Sullivan Jr.
Pair of Unusual GCN5 Histone Acetyltransferases and ADA2 Homologues in the Protozoan Parasite Toxoplasma gondii
Eukaryot. Cell,
January 1, 2006;
5(1):
62 - 76.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, L. Rizhsky, H. Liang, J. Shuman, V. Shulaev, and R. Mittler
Enhanced Tolerance to Environmental Stress in Transgenic Plants Expressing the Transcriptional Coactivator Multiprotein Bridging Factor 1c
Plant Physiology,
November 1, 2005;
139(3):
1313 - 1322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Xin, Y. Zhao, and Z.-L. Zheng
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Plant Physiology,
November 1, 2005;
139(3):
1350 - 1365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Pankotai, O. Komonyi, L. Bodai, Z. Ujfaludi, S. Muratoglu, A. Ciurciu, L. Tora, J. Szabad, and I. Boros
The Homologous Drosophila Transcriptional Adaptors ADA2a and ADA2b Are both Required for Normal Development but Have Different Functions
Mol. Cell. Biol.,
September 15, 2005;
25(18):
8215 - 8227.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Carre, D. Szymczak, J. Pidoux, and C. Antoniewski
The Histone H3 Acetylase dGcn5 Is a Key Player in Drosophila melanogaster Metamorphosis
Mol. Cell. Biol.,
September 15, 2005;
25(18):
8228 - 8238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Nelissen, D. Fleury, L. Bruno, P. Robles, L. De Veylder, J. Traas, J. L. Micol, M. Van Montagu, D. Inze, and M. Van Lijsebettens
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PNAS,
May 24, 2005;
102(21):
7754 - 7759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Zhou, L. Zhang, J. Duan, B. Miki, and K. Wu
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PLANT CELL,
April 1, 2005;
17(4):
1196 - 1204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Stekel, D. Sarti, V. Trevino, L. Zhang, M. Salmon, C. D. Buckley, M. Stevens, M. J. Pallen, C. Penn, and F. Falciani
Analysis of host response to bacterial infection using error model based gene expression microarray experiments
Nucleic Acids Res.,
March 30, 2005;
33(6):
e53 - e53.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Bhatti and W. J. Sullivan Jr.
Histone Acetylase GCN5 Enters the Nucleus via Importin-{alpha} in Protozoan Parasite Toxoplasma gondii
J. Biol. Chem.,
February 18, 2005;
280(7):
5902 - 5908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Furumoto, Y. Tamada, A. Izumida, H. Nakatani, S. Hata, and K. Izui
Abundant Expression in Vascular Tissue of Plant TAF10, an Orthologous Gene for TATA Box-binding Protein-associated Factor 10, in Flaveria trinervia and Abnormal Morphology of Arabidopsis thaliana Transformants on its Overexpression
Plant Cell Physiol.,
January 15, 2005;
46(1):
108 - 117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bertrand, M. Benhamed, Y.-F. Li, M. Ayadi, G. Lemonnier, J.-P. Renou, M. Delarue, and D.-X. Zhou
Arabidopsis HAF2 Gene Encoding TATA-binding Protein (TBP)-associated Factor TAF1, Is Required to Integrate Light Signals to Regulate Gene Expression and Growth
J. Biol. Chem.,
January 14, 2005;
280(2):
1465 - 1473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Browse and B. M. Lange
Counting the cost of a cold-blooded life: Metabolomics of cold acclimation
PNAS,
October 19, 2004;
101(42):
14996 - 14997.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Cook, S. Fowler, O. Fiehn, and M. F. Thomashow
From The Cover: A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis
PNAS,
October 19, 2004;
101(42):
15243 - 15248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Farrona, L. Hurtado, J. L. Bowman, and J. C. Reyes
The Arabidopsis thaliana SNF2 homolog AtBRM controls shoot development and flowering
Development,
October 15, 2004;
131(20):
4965 - 4975.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Koiwa, S. Hausmann, W. Y. Bang, A. Ueda, N. Kondo, A. Hiraguri, T. Fukuhara, J. D. Bahk, D.-J. Yun, R. A. Bressan, et al.
Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases
PNAS,
October 5, 2004;
101(40):
14539 - 14544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bertrand, C. Bergounioux, S. Domenichini, M. Delarue, and D.-X. Zhou
Arabidopsis Histone Acetyltransferase AtGCN5 Regulates the Floral Meristem Activity through the WUSCHEL/AGAMOUS Pathway
J. Biol. Chem.,
July 18, 2003;
278(30):
28246 - 28251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
