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First published online February 21, 2003; 10.1105/tpc.009258

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The Plant Cell, Vol. 15, 732-744, March 2003, Copyright © 2003,
American Society of Plant Biologists

Recognition Specificity and RAR1/SGT1 Dependence in Barley Mla Disease Resistance Genes to the Powdery Mildew Fungus

Qian-Hua Shen1,a,b, Fasong Zhou1,2,b, Stephane Bieri1,a, Thomas Haizelb, Ken Shirasub and Paul Schulze-Lefert3,a,b

a Max-Planck-Institut für Züchtungsforschung, Department of Plant–Microbe Interactions, Carl-von-Linné-Weg 10, D-50829 Köln, Germany
b The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom

3 To whom correspondence should be addressed. E-mail schlef{at}mpiz-koeln.mpg.de; fax 49-221-5062313

A large number of resistance specificities to the powdery mildew fungus Blumeria graminis f. sp. hordei map to the barley Mla locus. This complex locus harbors multiple members of three distantly related gene families that encode proteins that contain an N-terminal coiled-coil (CC) structure, a central nucleotide binding (NB) site, a Leu-rich repeat (LRR) region, and a C-terminal non-LRR (CT) region. We identified Mla12, which encodes a CC-NB-LRR-CT protein that shares 89 and 92% identical residues with the known proteins MLA1 and MLA6. Slow Mla12-triggered resistance was altered dramatically to a rapid response by overexpression of Mla12. A series of reciprocal domains swaps between MLA1 and MLA6 identified in each protein recognition domain for cognate powdery mildew fungus avirulence genes (AvrMla1 and AvrMla6). These domains were within different but overlapping LRR regions and the CT part. Unexpectedly, MLA chimeras that confer AvrMla6 recognition exhibited markedly different dependence on Rar1, a gene required for the function of some but not all Mla resistance specificities. Furthermore, uncoupling of MLA6-specific function from RAR1 also uncoupled the response from SGT1, a protein known to associate physically with RAR1. Our findings suggest that differences in the degree of RAR1 dependence of different MLA immunity responses are determined by intrinsic properties of MLA variants and place RAR1/SGT1 activity downstream of and/or coincident with the action of resistance protein–containing recognition complexes.




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