This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Seo, S. Articles by Ohashi, Y. Search for Related Content PubMed PubMed Citation Articles by Seo, S. Articles by Ohashi, Y. Agricola Articles by Seo, S. Articles by Ohashi, Y.

A Diterpene as an Endogenous Signal for the Activation of Defense Responses to Infection with Tobacco mosaic virus and Wounding in Tobacco

^a Plant Physiology Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
^b Core Research of Science and Technology, Japan Science and Technology, Chiyoda-ku, Tokyo 101-0062, Japan
^c RIKEN, The Institute of Physical and Chemical Research, Wako-shi, Saitama 351-0198, Japan

¹ To whom correspondence should be addressed. E-mail yohashi{at}affrc.go.jp; fax 81-298-38-7469

In pathogen-infected or wounded tobacco plants, the activation of wound-induced protein kinase (WIPK), a tobacco mitogen-activated protein kinase, has been implicated in the defense response. However, no endogenous signal responsible for the activation has been identified. A WIPK-activating substance was isolated from tobacco leaves and identified as (11E,13E)-labda-11,13-diene-8,15-diol, designated WAF-1. When applied in nanomolar concentrations to leaves, either natural WAF-1 or chemically synthesized WAF-1 activated WIPK as well as salicylic acid–induced protein kinase, a tobacco mitogen-activated protein kinase, and enhanced the accumulation of transcripts of wound- and pathogen-inducible defense-related genes. Quantitative analysis of endogenous WAF-1 revealed that levels increased rapidly in leaves during a hypersensitive response to Tobacco mosaic virus (TMV) and after wounding. Furthermore, treatment of leaves with WAF-1 resulted in enhanced resistance to TMV infection. These results suggest that WAF-1 functions as an endogenous signal to mediate the defense responses of tobacco plants to TMV infection and wounding.

This article has been cited by other articles:

				F. Takahashi, R. Yoshida, K. Ichimura, T. Mizoguchi, S. Seo, M. Yonezawa, K. Maruyama, K. Yamaguchi-Shinozaki, and K. Shinozaki The Mitogen-Activated Protein Kinase Cascade MKK3-MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in Arabidopsis PLANT CELL, March 1, 2007; 19(3): 805 - 818. [Abstract] [Full Text] [PDF]

				Y. Kobae, T. Sekino, H. Yoshioka, T. Nakagawa, E. Martinoia, and M. Maeshima Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection Plant Cell Physiol., March 1, 2006; 47(3): 309 - 318. [Abstract] [Full Text] [PDF]

				G. He, Y. Tarui, and M. Iino A Novel Receptor Kinase Involved in Jasmonate-mediated Wound and Phytochrome Signaling in Maize Coleoptiles Plant Cell Physiol., June 1, 2005; 46(6): 870 - 883. [Abstract] [Full Text] [PDF]

				G. J. WAGNER, E. WANG, and R. W. SHEPHERD New Approaches for Studying and Exploiting an Old Protuberance, the Plant Trichome Ann. Bot., January 1, 2004; 93(1): 3 - 11. [Abstract] [Full Text] [PDF]

				E. J. Campbell, P. M. Schenk, K. Kazan, I. A.M.A. Penninckx, J. P. Anderson, D. J. Maclean, B. P.A. Cammue, P. R. Ebert, and J. M. Manners Pathogen-Responsive Expression of a Putative ATP-Binding Cassette Transporter Gene Conferring Resistance to the Diterpenoid Sclareol Is Regulated by Multiple Defense Signaling Pathways in Arabidopsis Plant Physiology, November 1, 2003; 133(3): 1272 - 1284. [Abstract] [Full Text] [PDF]