Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online March 4, 2003; 10.1105/tpc.010249

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
15/4/874    most recent
tpc.010249v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Singh, M.
Right arrow Articles by Brutnell, T. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Singh, M.
Right arrow Articles by Brutnell, T. P.
Agricola
Right arrow Articles by Singh, M.
Right arrow Articles by Brutnell, T. P.
The Plant Cell, Vol. 15, 874-884, April 2003, Copyright © 2003,
American Society of Plant Biologists

Activator Mutagenesis of the Pink scutellum1/viviparous7 Locus of Maize

Manjit Singha, Paul E. Lewisa, Kristine Hardemanb, Ling Baia, Jocelyn K. C. Rosec, Michael Mazourekc, Paul Chometb and Thomas P. Brutnell1,a

a Boyce Thompson Institute, Cornell University, Ithaca, New York 14853
b Monsanto/Mystic Research, Mystic, Connecticut 06355
c Department of Plant Biology, Cornell University, Ithaca, New York 14853

1 To whom correspondence should be addressed. E-mail tpb8{at}cornell.edu; fax 607-254-1242

The transposable elements Activator/Dissociation (Ac/Ds) were first discovered in maize, yet they have not been used extensively in their native host for gene-tagging experiments. This can be attributed largely to the low forward mutation rate and the propensity for closely linked transpositions associated with Ac and its nonautonomous deletion derivative Ds. To overcome these limitations, we are developing a series of nearly isogenic maize lines, each with a single active Ac element positioned at a well-defined location. These Ac elements are distributed at 10- to 20-centimorgan intervals throughout the genome for use in regional mutagenesis. Here, we demonstrate the utility of this Ac-based gene-tagging approach through the targeted mutagenesis of the pink scutellum1/viviparous7 (ps1/vp7) locus. Using a novel PCR-based technique, the Ps1 gene was cloned and Ac elements positioned precisely in each of the seven alleles recovered. The Ps1 gene is predicted to encode lycopene {beta}-cyclase and is necessary for the accumulation of both abscisic acid and the carotenoid zeaxanthin in mature maize embryos. This study demonstrates the utility of an Ac mutagenesis program to efficiently generate allelic diversity at closely linked loci in maize.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Covshoff, W. Majeran, P. Liu, J. M. Kolkman, K. J. van Wijk, and T. P. Brutnell
Deregulation of Maize C4 Photosynthetic Development in a Mesophyll Cell-Defective Mutant
Plant Physiology, April 1, 2008; 146(4): 1469 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. J. Conrad, L. Bai, K. Ahern, K. Dusinberre, D. P. Kane, and T. P. Brutnell
State II Dissociation Element Formation Following Activator Excision in Maize
Genetics, October 1, 2007; 177(2): 737 - 747.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. Bai, M. Singh, L. Pitt, M. Sweeney, and T. P. Brutnell
Generating Novel Allelic Variation Through Activator Insertional Mutagenesis in Maize
Genetics, March 1, 2007; 175(3): 981 - 992.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
W. Yu, J. C. Lamb, F. Han, and J. A. Birchler
Cytological Visualization of DNA Transposons and Their Transposition Pattern in Somatic Cells of Maize
Genetics, January 1, 2007; 175(1): 31 - 39.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. J. Conrad and T. P. Brutnell
Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize
Genetics, December 1, 2005; 171(4): 1999 - 2012.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. M. Kolkman, L. J. Conrad, P. R. Farmer, K. Hardeman, K. R. Ahern, P. E. Lewis, R. J. H. Sawers, S. Lebejko, P. Chomet, and T. P. Brutnell
Distribution of Activator (Ac) Throughout the Maize Genome for Use in Regional Mutagenesis
Genetics, February 1, 2005; 169(2): 981 - 995.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. J.H. Sawers, P. J. Linley, J. F. Gutierrez-Marcos, T. Delli-Bovi, P. R. Farmer, T. Kohchi, M. J. Terry, and T. P. Brutnell
The Elm1 (ZmHy2) Gene of Maize Encodes a Phytochromobilin Synthase
Plant Physiology, September 1, 2004; 136(1): 2771 - 2781.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. M. Settles, S. Latshaw, and D. R. McCarty
Molecular analysis of high-copy insertion sites in maize
Nucleic Acids Res., April 1, 2004; 32(6): e54 - e54.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. M. Costa, J. F. Gutierrez-Marcos, T. P. Brutnell, A. J. Greenland, and H. G. Dickinson
The globby1-1 (glo1-1) mutation disrupts nuclear and cell division in the developing maize seed causing alterations in endosperm cell fate and tissue differentiation
Development, October 15, 2003; 130(20): 5009 - 5017.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists