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First published online March 4, 2003; 10.1105/tpc.009217

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The Plant Cell, Vol. 15, 965-980, April 2003, Copyright © 2003,
American Society of Plant Biologists

The Pleiotropic Role of the 26S Proteasome Subunit RPN10 in Arabidopsis Growth and Development Supports a Substrate-Specific Function in Abscisic Acid Signaling

Jan Smallea, Jasmina Kurepaa, Peizhen Yanga, Thomas J. Emborga, Elena Babiychukb, Sergei Kushnirb and Richard D. Vierstra1,a

a Department of Horticulture and Cellular and Molecular Biology Program, University of Wisconsin–Madison, Madison, Wisconsin 53706
b Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

1 To whom correspondence should be addressed. E-mail vierstra{at}facstaff.wisc.edu; fax 608-262-4743

The 26S proteasome is an essential protease complex responsible for removing most short-lived intracellular proteins, especially those modified with polyubiquitin chains. We show here that an Arabidopsis mutant expressing an altered RPN10 subunit exhibited a pleiotropic phenotype consistent with specific changes in 26S proteasome function. rpn10-1 plants displayed reduced seed germination, growth rate, stamen number, genetic transmission through the male gamete, and hormone-induced cell division, which can be explained partially by a constitutive downregulation of the key cell cycle gene CDKA;1. rpn10-1 also was more sensitive to abscisic acid (ABA), salt, and sucrose stress and to DNA-damaging agents and had decreased sensitivity to cytokinin and auxin. Most of the phenotypes can be explained by a hypersensitivity to ABA, which is reflected at the molecular level by the selective stabilization of the short-lived ABA-signaling protein ABI5. Collectively, these results indicate that RPN10 affects a number of regulatory processes in Arabidopsis likely by directing specific proteins to the 26S proteasome for degradation. A particularly important role may be in regulating the responses to signals promulgated by ABA.




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