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First published online April 3, 2003; 10.1105/tpc.010413

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The Plant Cell, Vol. 15, 1095-1110, May 2003, Copyright © 2003,
American Society of Plant Biologists

Dominant-Negative Receptor Uncovers Redundancy in the Arabidopsis ERECTA Leucine-Rich Repeat Receptor–Like Kinase Signaling Pathway That Regulates Organ Shape

Elena D. Shpaka, Michael B. Lakemana and Keiko U. Torii1,a,b

a Department of Biology, University of Washington, Seattle, Washington 98195-1800
b CREST, Japan Science and Technology Corporation, Kawaguchi, Saitama 322-0012, Japan

1 To whom correspondence should be addressed. E-mail ktorii{at}u.washington.edu; fax 1-206-685-1728

Arabidopsis ERECTA, a Leu-rich repeat receptor-like Ser/Thr kinase (LRR-RLK), regulates organ shape and inflorescence architecture. Here, we show that a truncated ERECTA protein that lacks the cytoplasmic kinase domain ({Delta}Kinase) confers dominant-negative effects when expressed under the control of the native ERECTA promoter and terminator. Transgenic plants expressing {Delta}Kinase displayed phenotypes, including compact inflorescence and short, blunt siliques, that are characteristic of loss-of-function erecta mutant plants. The {Delta}Kinase fragment migrated as a stable ~400-kD protein complex in the complete absence of the endogenous ERECTA protein and significantly exaggerated the growth defects of the null erecta plants. A functional LRR domain of {Delta}Kinase was required for dominant-negative effects. Accumulation of {Delta}Kinase did not interfere with another LRR-RLK signaling pathway (CLAVATA1), which operates in the same cells as ERECTA but has a distinct biological function. Both the erecta mutation and {Delta}Kinase expression conferred a lesser number of large, disorganized, and expanded cortex cells, which are associated with an increased level of somatic endoploidy. These findings suggest that functionally redundant RLK signaling pathways, including ERECTA, are required to fine-tune the proliferation and growth of cells in the same tissue type during Arabidopsis organogenesis.




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