This Article Full Text Full Text (PDF) All Versions of this Article: 15/6/1347    most recent tpc.009902v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Lois, L. M. Articles by Chua, N.-H. Search for Related Content PubMed PubMed Citation Articles by Lois, L. M. Articles by Chua, N.-H. Agricola Articles by Lois, L. M. Articles by Chua, N.-H.

Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis

^a Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New York 10021-6399
^b Biochemistry Department, Weill Medical College of Cornell University, New York, New York 10021

² To whom correspondence should be addressed. E-mail chua{at}mail.rockefeller.edu; _fax 212-327-8327

Post-translational modification of proteins by small polypeptides, such as ubiquitin, has emerged as a common and important mechanism for regulating protein function. Small ubiquitin-like modifier (SUMO) is a small protein that is structurally related to but functionally different from ubiquitin. We report the identification and functional analysis of AtSUMO1, AtSUMO2, and AtSCE1a as components of the SUMO conjugation (sumoylation) pathway in Arabidopsis. In yeast-two hybrid assays, AtSUMO1/2 interacts specifically with a SUMO-conjugating enzyme but not with a ubiquitin-conjugating enzyme. AtSCE1a, the Arabidopsis SUMO-conjugating enzyme ortholog, conjugates SUMO to RanGAP in vitro. AtSUMO1/2 and AtSCE1a colocalize at the nucleus, and AtSUMO1/2 are conjugated to endogenous SUMO targets in vivo. Analysis of transgenic plants showed that overexpression of AtSUMO1/2 does not have any obvious effect in general plant development, but increased sumoylation levels attenuate abscisic acid (ABA)–mediated growth inhibition and amplify the induction of ABA- and stress-responsive genes such as RD29A. Reduction of AtSCE1a expression levels accentuates ABA-mediated growth inhibition. Our results suggest a role for SUMO in the modulation of the ABA signal transduction pathway.

This article has been cited by other articles:

				H. Ullah, E. L. Scappini, A. F. Moon, L. V. Williams, D. L. Armstrong, and L. C. Pedersen Structure of a signal transduction regulator, RACK1, from Arabidopsis thaliana Protein Sci., October 1, 2008; 17(10): 1771 - 1780. [Abstract] [Full Text] [PDF]

				Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim Hormone- and light-regulated nucleocytoplasmic transport in plants: current status J. Exp. Bot., September 1, 2008; 59(12): 3229 - 3245. [Abstract] [Full Text] [PDF]

				Y. Wang, I. Ladunga, A. R. Miller, K. M. Horken, T. Plucinak, D. P. Weeks, and C. P. Bailey The Small Ubiquitin-Like Modifier (SUMO) and SUMO-Conjugating System of Chlamydomonas reinhardtii Genetics, May 1, 2008; 179(1): 177 - 192. [Abstract] [Full Text] [PDF]

				R. Catala, J. Ouyang, I. A. Abreu, Y. Hu, H. Seo, X. Zhang, and N.-H. Chua The Arabidopsis E3 SUMO Ligase SIZ1 Regulates Plant Growth and Drought Responses PLANT CELL, September 1, 2007; 19(9): 2952 - 2966. [Abstract] [Full Text] [PDF]

				S. A. Saracco, M. J. Miller, J. Kurepa, and R. D. Vierstra Genetic Analysis of SUMOylation in Arabidopsis: Conjugation of SUMO1 and SUMO2 to Nuclear Proteins Is Essential Plant Physiology, September 1, 2007; 145(1): 119 - 134. [Abstract] [Full Text] [PDF]

				K. Miura, J. B. Jin, J. Lee, C. Y. Yoo, V. Stirm, T. Miura, E. N. Ashworth, R. A. Bressan, D.-J. Yun, and P. M. Hasegawa SIZ1-Mediated Sumoylation of ICE1 Controls CBF3/DREB1A Expression and Freezing Tolerance in Arabidopsis PLANT CELL, April 1, 2007; 19(4): 1403 - 1414. [Abstract] [Full Text] [PDF]

				T. Colby, A. Matthai, A. Boeckelmann, and H.-P. Stuible SUMO-Conjugating and SUMO-Deconjugating Enzymes from Arabidopsis Plant Physiology, September 1, 2006; 142(1): 318 - 332. [Abstract] [Full Text] [PDF]

				D. R. Hoen, K. C. Park, N. Elrouby, Z. Yu, N. Mohabir, R. K. Cowan, and T. E. Bureau Transposon-Mediated Expansion and Diversification of a Family of ULP-like Genes Mol. Biol. Evol., June 1, 2006; 23(6): 1254 - 1268. [Abstract] [Full Text] [PDF]

				K. Miura, A. Rus, A. Sharkhuu, S. Yokoi, A. S. Karthikeyan, K. G. Raghothama, D. Baek, Y. D. Koo, J. B. Jin, R. A. Bressan, et al. The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses PNAS, May 24, 2005; 102(21): 7760 - 7765. [Abstract] [Full Text] [PDF]

				F. M. Carland and T. Nelson COTYLEDON VASCULAR PATTERN2-Mediated Inositol (1,4,5) Triphosphate Signal Transduction Is Essential for Closed Venation Patterns of Arabidopsis Foliar Organs PLANT CELL, May 1, 2004; 16(5): 1263 - 1275. [Abstract] [Full Text] [PDF]

				A. G. Castillo, L. J. Kong, L. Hanley-Bowdoin, and E. R. Bejarano Interaction between a Geminivirus Replication Protein and the Plant Sumoylation System J. Virol., March 15, 2004; 78(6): 2758 - 2769. [Abstract] [Full Text] [PDF]

				G. Murtas, P. H. Reeves, Y.-F. Fu, I. Bancroft, C. Dean, and G. Coupland A Nuclear Protease Required for Flowering-Time Regulation in Arabidopsis Reduces the Abundance of SMALL UBIQUITIN-RELATED MODIFIER Conjugates PLANT CELL, October 1, 2003; 15(10): 2308 - 2319. [Abstract] [Full Text] [PDF]