This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 15/6/1455    most recent tpc.011452v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (38) Citing Articles via Google Scholar Google Scholar Articles by Kusaba, M. Articles by Nishio, T. Search for Related Content PubMed PubMed Citation Articles by Kusaba, M. Articles by Nishio, T. Agricola Articles by Kusaba, M. Articles by Nishio, T.

Low glutelin content1: A Dominant Mutation That Suppresses the Glutelin Multigene Family via RNA Silencing in Rice

^a Institute of Radiation Breeding, National Institute of Agrobiological Sciences, Ohmiya-machi, Naka-gun, Ibaraki 319-2293, Japan
^b National Agricultural Research Center for the Western Region, National Agricultural Research Organization, Fukuyama, Hiroshima 721-8514, Japan
^c Kihara Institute for Biological Research, Yokohama City University, Maioka, Totsuka-ku, Yokohama 244-0813, Japan
^d Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan

¹ To whom correspondence should be addressed. E-mail kusaba{at}affrc.go.jp; fax 81-295-53-1075

Low glutelin content1 (Lgc1) is a dominant mutation that reduces glutelin content in rice grains. Glutelin is a major seed storage protein encoded by a multigene family. RNA gel blot and reverse transcriptase–mediated PCR analyses revealed that Lgc1 acts at the mRNA level in a similarity-dependent manner. In Lgc1 homozygotes, there is a 3.5-kb deletion between two highly similar glutelin genes that forms a tail-to-tail inverted repeat, which might produce a double-stranded RNA molecule, a potent inducer of RNA silencing. The hypothesis that Lgc1 suppresses glutelin expression via RNA silencing is supported by transgenic analysis using this Lgc1 candidate region, by reporter gene analysis, and by the detection of small interfering RNAs. In this context, Lgc1 provides an interesting example of RNA silencing occurring among genes that exhibit various levels of similarity to an RNA-silencing–inducing gene. Possible mechanisms for gene silencing of the glutelin multigene family by Lgc1 are discussed.

This article has been cited by other articles:

				L. Q. Qu, Y. P. Xing, W. X. Liu, X. P. Xu, and Y. R. Song Expression pattern and activity of six glutelin gene promoters in transgenic rice J. Exp. Bot., June 1, 2008; 59(9): 2417 - 2424. [Abstract] [Full Text] [PDF]

				A. Kanazawa, M. O'Dell, and R. P. Hellens Epigenetic Inactivation of Chalcone Synthase-A Transgene Transcription in Petunia Leads to a Reversion of the Post-Transcriptional Gene Silencing Phenotype Plant Cell Physiol., April 1, 2007; 48(4): 638 - 647. [Abstract] [Full Text] [PDF]

				M. Kusaba, H. Ito, R. Morita, S. Iida, Y. Sato, M. Fujimoto, S. Kawasaki, R. Tanaka, H. Hirochika, M. Nishimura, et al. Rice NON-YELLOW COLORING1 Is Involved in Light-Harvesting Complex II and Grana Degradation during Leaf Senescence PLANT CELL, April 1, 2007; 19(4): 1362 - 1375. [Abstract] [Full Text] [PDF]

				M. Koseki, K. Goto, C. Masuta, and A. Kanazawa The Star-type Color Pattern in Petunia hybrida 'Red Star' Flowers is Induced by Sequence-Specific Degradation of Chalcone Synthase RNA Plant Cell Physiol., November 1, 2005; 46(11): 1879 - 1883. [Abstract] [Full Text] [PDF]

				C. B. Della Vedova, R. Lorbiecke, H. Kirsch, M. B. Schulte, K. Scheets, L. M. Borchert, B. E. Scheffler, U. Wienand, K. C. Cone, and J. A. Birchler The Dominant Inhibitory Chalcone Synthase Allele C2-Idf (Inhibitor diffuse) From Zea mays (L.) Acts via an Endogenous RNA Silencing Mechanism Genetics, August 1, 2005; 170(4): 1989 - 2002. [Abstract] [Full Text] [PDF]

				B. J. Townsend, A. Poole, C. J. Blake, and D. J. Llewellyn Antisense Suppression of a (+)-{delta}-Cadinene Synthase Gene in Cotton Prevents the Induction of This Defense Response Gene during Bacterial Blight Infection But Not Its Constitutive Expression Plant Physiology, May 1, 2005; 138(1): 516 - 528. [Abstract] [Full Text] [PDF]

				B. C.W. Crawford, U. Nath, R. Carpenter, and E. S. Coen CINCINNATA Controls Both Cell Differentiation and Growth in Petal Lobes and Leaves of Antirrhinum Plant Physiology, May 1, 2004; 135(1): 244 - 253. [Abstract] [Full Text] [PDF]

				D. Miki and K. Shimamoto Simple RNAi Vectors for Stable and Transient Suppression of Gene Function in Rice Plant Cell Physiol., April 15, 2004; 45(4): 490 - 495. [Abstract] [Full Text] [PDF]

				M. Senda, C. Masuta, S. Ohnishi, K. Goto, A. Kasai, T. Sano, J.-S. Hong, and S. MacFarlane Patterning of Virus-Infected Glycine max Seed Coat Is Associated with Suppression of Endogenous Silencing of Chalcone Synthase Genes PLANT CELL, April 1, 2004; 16(4): 807 - 818. [Abstract] [Full Text] [PDF]