Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online July 3, 2003; 10.1105/tpc.012955

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
15/8/1859    most recent
tpc.012955v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (54)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kubis, S.
Right arrow Articles by Jarvis, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kubis, S.
Right arrow Articles by Jarvis, P.
Agricola
Right arrow Articles by Kubis, S.
Right arrow Articles by Jarvis, P.
The Plant Cell, Vol. 15, 1859-1871, August 2003, Copyright © 2003,
American Society of Plant Biologists

The Arabidopsis ppi1 Mutant Is Specifically Defective in the Expression, Chloroplast Import, and Accumulation of Photosynthetic Proteins

Sybille Kubisa, Amy Baldwina, Ramesh Patela, Azam Razzaqb, Paul Dupreeb, Kathryn Lilleyb, Joachim Kurthc, Dario Leisterc and Paul Jarvis1,a

a Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom
b Cambridge Centre for Proteomics, University of Cambridge, Cambridge CB2 1QW, United Kingdom
c Department for Plant Breeding and Yield Physiology, Max-Planck-Institute for Plant Breeding Research, D-50829 Cologne, Germany

1 To whom correspondence should be addressed. E-mail rpj3{at}le.ac.uk; fax 44-116-252-3330

The import of nucleus-encoded proteins into chloroplasts is mediated by translocon complexes in the envelope membranes. A component of the translocon in the outer envelope membrane, Toc34, is encoded in Arabidopsis by two homologous genes, atTOC33 and atTOC34. Whereas atTOC34 displays relatively uniform expression throughout development, atTOC33 is strongly upregulated in rapidly growing, photosynthetic tissues. To understand the reason for the existence of these two related genes, we characterized the atTOC33 knockout mutant ppi1. Immunoblotting and proteomics revealed that components of the photosynthetic apparatus are deficient in ppi1 chloroplasts and that nonphotosynthetic chloroplast proteins are unchanged or enriched slightly. Furthermore, DNA array analysis of 3292 transcripts revealed that photosynthetic genes are moderately, but specifically, downregulated in ppi1. Proteome differences in ppi1 could be correlated with protein import rates: ppi1 chloroplasts imported the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit and 33-kD oxygen-evolving complex precursors at significantly reduced rates, but the import of a 50S ribosomal subunit precursor was largely unaffected. The ppi1 import defect occurred at the level of preprotein binding, which is consistent with a role for atToc33 during preprotein recognition. The data suggest that atToc33 is involved preferentially in the import of photosynthetic proteins and, by extension, that atToc34 is involved in the import of nonphotosynthetic chloroplast proteins.




This article has been cited by other articles:


Home page
 JCBHome page
F. Wang, B. Agne, F. Kessler, and D. J. Schnell
The role of GTP binding and hydrolysis at the atToc159 preprotein receptor during protein import into chloroplasts
J. Cell Biol., October 6, 2008; 183(1): 87 - 99.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
C.-S. Lin, N.-T. Liu, D.-C. Liao, J.-S. Yu, C.-H. Tsao, C.-H. Lin, C.-W. Sun, W.-N. Jane, H. S. Tsay, J. Jian-Wei Chen, et al.
Differential Protein Expression of Two Photosystem II Subunits, PsbO and PsbP, in an Albino Mutant of Bambusa edulis with Chloroplast DNA Aberration
J. Amer. Soc. Hort. Sci., March 1, 2008; 133(2): 270 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P.-H. Su and H.-m. Li
Arabidopsis Stromal 70-kD Heat Shock Proteins Are Essential for Plant Development and Important for Thermotolerance of Germinating Seeds
Plant Physiology, March 1, 2008; 146(3): 1231 - 1241.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Bedard, S. Kubis, S. Bimanadham, and P. Jarvis
Functional Similarity between the Chloroplast Translocon Component, Tic40, and the Human Co-chaperone, Hsp70-interacting Protein (Hip)
J. Biol. Chem., July 20, 2007; 282(29): 21404 - 21414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-H. Yeh, M. M. Kesavulu, H.-m. Li, S.-Z. Wu, Y.-J. Sun, E. H. E. Konozy, and C.-D. Hsiao
Dimerization Is Important for the GTPase Activity of Chloroplast Translocon Components atToc33 and psToc159
J. Biol. Chem., May 4, 2007; 282(18): 13845 - 13853.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. E. Reddick, M. D. Vaughn, S. J. Wright, I. M. Campbell, and B. D. Bruce
In Vitro Comparative Kinetic Analysis of the Chloroplast Toc GTPases
J. Biol. Chem., April 13, 2007; 282(15): 11410 - 11426.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Kleffmann, A. von Zychlinski, D. Russenberger, M. Hirsch-Hoffmann, P. Gehrig, W. Gruissem, and S. Baginsky
Proteome Dynamics during Plastid Differentiation in Rice
Plant Physiology, February 1, 2007; 143(2): 912 - 923.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Lopez-Juez
Plastid biogenesis, between light and shadows
J. Exp. Bot., January 1, 2007; 58(1): 11 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Sauret-Gueto, P. Botella-Pavia, U. Flores-Perez, J. F. Martinez-Garcia, C. San Roman, P. Leon, A. Boronat, and M. Rodriguez-Concepcion
Plastid Cues Posttranscriptionally Regulate the Accumulation of Key Enzymes of the Methylerythritol Phosphate Pathway in Arabidopsis
Plant Physiology, May 1, 2006; 141(1): 75 - 84.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. S Lilley and P. Dupree
Methods of quantitative proteomics and their application to plant organelle characterization
J. Exp. Bot., April 1, 2006; 57(7): 1493 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Bedard and P. Jarvis
Recognition and envelope translocation of chloroplast preproteins
J. Exp. Bot., September 1, 2005; 56(419): 2287 - 2320.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Baldwin, A. Wardle, R. Patel, P. Dudley, S. K. Park, D. Twell, K. Inoue, and P. Jarvis
A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family
Plant Physiology, June 1, 2005; 138(2): 715 - 733.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Fey, R. Wagner, K. Brautigam, M. Wirtz, R. Hell, A. Dietzmann, D. Leister, R. Oelmuller, and T. Pfannschmidt
Retrograde Plastid Redox Signals in the Expression of Nuclear Genes for Chloroplast Proteins of Arabidopsis thaliana
J. Biol. Chem., February 18, 2005; 280(7): 5318 - 5328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ko, D. Taylor, P. Argenton, J. Innes, B. Pedram, F. Seibert, A. Granell, and Z. Ko
Evidence That the Plastid Translocon Tic40 Components Possess Modulating Capabilities
J. Biol. Chem., January 7, 2005; 280(1): 215 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kubis, R. Patel, J. Combe, J. Bedard, S. Kovacheva, K. Lilley, A. Biehl, D. Leister, G. Rios, C. Koncz, et al.
Functional Specialization amongst the Arabidopsis Toc159 Family of Chloroplast Protein Import Receptors
PLANT CELL, August 1, 2004; 16(8): 2059 - 2077.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Ivanova, M. D. Smith, K. Chen, and D. J. Schnell
Members of the Toc159 Import Receptor Family Represent Distinct Pathways for Protein Targeting to Plastids
Mol. Biol. Cell, July 1, 2004; 15(7): 3379 - 3392.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Baginsky and W. Gruissem
Chloroplast proteomics: potentials and challenges
J. Exp. Bot., June 1, 2004; 55(400): 1213 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Chew, J. Whelan, and A. H. Millar
Molecular Definition of the Ascorbate-Glutathione Cycle in Arabidopsis Mitochondria Reveals Dual Targeting of Antioxidant Defenses in Plants
J. Biol. Chem., November 21, 2003; 278(47): 46869 - 46877.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2003 by the American Society of Plant Biologists