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The Presence of a Chromatin Boundary Appears to Shield a Transgene in Tobacco from RNA Silencing

^a Plant Research International, Wageningen University and Research Centre, NL-6700AA Wageningen, The Netherlands
^b Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, SK-94901 Nitra, Slovak Republic

¹ To whom correspondence should be addressed. E-mail j.p.h.nap{at}plant.wag-ur.nl; fax 31-317-418094

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site–specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous.

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