Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online October 14, 2004; 10.1105/tpc.104.025999

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
16/11/2984    most recent
tpc.104.025999v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gusmaroli, G.
Right arrow Articles by Deng, X. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gusmaroli, G.
Right arrow Articles by Deng, X. W.
Agricola
Right arrow Articles by Gusmaroli, G.
Right arrow Articles by Deng, X. W.
The Plant Cell 16:2984-3001 (2004)
© 2004 American Society of Plant Biologists

The Arabidopsis CSN5A and CSN5B Subunits Are Present in Distinct COP9 Signalosome Complexes, and Mutations in Their JAMM Domains Exhibit Differential Dominant Negative Effects on Development

Giuliana Gusmaroli, Suhua Feng and Xing Wang Deng1

Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104

1 To whom correspondence should be addressed. E-mail xingwang.deng{at}yale.edu; fax 203-432-5726.

The COP9 signalosome (CSN) is an evolutionarily conserved multisubunit protein complex involved in a variety of signaling and developmental processes through the regulation of protein ubiquitination and degradation. A known biochemical role attributed to CSN is a metalloprotease activity responsible for the derubylation of cullins, core components for several types of ubiquitin E3 ligases. The CSN's derubylation catalytic center resides in its subunit 5, which in Arabidopsis thaliana is encoded by two homologous genes, CSN5A and CSN5B. Here, we show that CSN5A and CSN5B subunits are assembled into distinct CSN complexes in vivo, which are present in drastically different abundances, with CSNCSN5A appearing to be the dominant one. Transgenic CSN5A and CSN5B proteins carrying a collection of single mutations in or surrounding the metalloprotease catalytic center are properly assembled into CSN complexes, but only mutations in CSN5A result in a pleiotropic dominant negative phenotype. The extent of phenotypic effects caused by mutations in CSN5A is reflected at the molecular level by impairment in Cullin1 derubylation. These results reveal that three key metal binding residues as well as two other amino acids outside the catalytic center play important roles in CSN derubylation activity. Taken together, our data provide physiological evidence on a positive role of CSN in the regulation of Arabidopsis SCF (for Skp1-Cullin-F-box) E3 ligases through RUB (for Related to Ubiquitin) deconjugation and highlight the unequal role that CSNCSN5A and CSNCSN5B play in controlling the cellular derubylation of cullins. The initial characterization of CSN5A and CSN5B insertion mutants further supports these findings and provides genetic evidence on their unequal role in plant development.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. M. N. Dohmann, M. P. Levesque, E. Isono, M. Schmid, and C. Schwechheimer
Auxin Responses in Mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome
Plant Physiology, July 1, 2008; 147(3): 1369 - 1379.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. M. N. Dohmann, M. P. Levesque, L. De Veylder, I. Reichardt, G. Jurgens, M. Schmid, and C. Schwechheimer
The Arabidopsis COP9 signalosome is essential for G2 phase progression and genomic stability
Development, June 1, 2008; 135(11): 2013 - 2022.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Busch, E. U. Schwier, K. Nahlik, O. Bayram, K. Helmstaedt, O. W. Draht, S. Krappmann, O. Valerius, W. N. Lipscomb, and G. H. Braus
An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation
PNAS, May 8, 2007; 104(19): 8089 - 8094.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. Gusmaroli, P. Figueroa, G. Serino, and X. W. Deng
Role of the MPN Subunits in COP9 Signalosome Assembly and Activity, and Their Regulatory Interaction with Arabidopsis Cullin3-Based E3 Ligases
PLANT CELL, February 1, 2007; 19(2): 564 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Chen, Y. Shen, X. Tang, L. Yu, J. Wang, L. Guo, Y. Zhang, H. Zhang, S. Feng, E. Strickland, et al.
Arabidopsis CULLIN4 Forms an E3 Ubiquitin Ligase with RBX1 and the CDD Complex in Mediating Light Control of Development
PLANT CELL, August 1, 2006; 18(8): 1991 - 2004.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. C. Hallstrom and J. R. Nevins
Jab1 is a specificity factor for E2F1-induced apoptosis
Genes & Dev., March 1, 2006; 20(5): 613 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. M.N. Dohmann, C. Kuhnle, and C. Schwechheimer
Loss of the CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Subunit 5 Is Sufficient to Cause the cop/det/fus Mutant Phenotype in Arabidopsis
PLANT CELL, July 1, 2005; 17(7): 1967 - 1978.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists