Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online October 14, 2004; 10.1105/tpc.104.024737

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
16/11/3069    most recent
tpc.104.024737v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swarup, R.
Right arrow Articles by Bennett, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swarup, R.
Right arrow Articles by Bennett, M. J.
Agricola
Right arrow Articles by Swarup, R.
Right arrow Articles by Bennett, M. J.
The Plant Cell 16:3069-3083 (2004)
© 2004 American Society of Plant Biologists

Structure-Function Analysis of the Presumptive Arabidopsis Auxin Permease AUX1{boxw}

Ranjan Swarupa,1, Joanna Kargula,1,2, Alan Marchanta,3, Daniel Zadika, Abidur Rahmanb,4, Rebecca Millsc, Anthony Yemma,d, Sean Maya, Lorraine Williamsc, Paul Millnere, Seiji Tsurumib, Ian Mooref, Richard Napierd, Ian D. Kerrg and Malcolm J. Bennetta,5

a School of Biosciences, Sutton Bonington Campus, University of Nottingham, United Kingdom
b Centre for Support to Research and Education Activities Isotope Division, Kobe University, Kobe, Japan
c School of Biological Sciences, University of Southampton, United Kingdom
d Warwick-HRI, University of Warwick, Wellesbourne, United Kingdom
e School of Biochemistry and Molecular Biology, University of Leeds, Leeds, United Kingdom
f Plant Sciences, University of Oxford, United Kingdom
g School of Biomedical Sciences, Queens Medical Centre, University of Nottingham, United Kingdom

5 To whom correspondence should be addressed. E-mail malcolm.bennett{at}nottingham.ac.uk; fax 0115-951-6331.

We have investigated the subcellular localization, the domain topology, and the amino acid residues that are critical for the function of the presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments and confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed that AUX1 colocalized with plasma membrane (PM) markers. Because of its PM localization, we were able to take advantage of the steep pH gradient that exists across the plant cell PM to investigate AUX1 topology using YFP as a pH-sensitive probe. The YFP-coding sequence was inserted in selected AUX1 hydrophilic loops to orient surface domains on either apoplastic or cytoplasmic faces of the PM based on the absence or presence of YFP fluorescence, respectively. We were able to demonstrate in conjunction with helix prediction programs that AUX1 represents a polytopic membrane protein composed of 11 transmembrane spanning domains. In parallel, a large aux1 allelic series containing null, partial-loss-of-function, and conditional mutations was characterized to identify the functionally important domains and amino acid residues within the AUX1 polypeptide. Whereas almost all partial-loss-of-function and null alleles cluster in the core permease region, the sole conditional allele aux1-7 modifies the function of the external C-terminal domain.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
D. J. Carrier, N. T. A. Bakar, R. Swarup, R. Callaghan, R. M. Napier, M. J. Bennett, and I. D. Kerr
The Binding of Auxin to the Arabidopsis Auxin Influx Transporter AUX1
Plant Physiology, September 1, 2008; 148(1): 529 - 535.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Robert, S. N. Chary, G. Drakakaki, S. Li, Z. Yang, N. V. Raikhel, and G. R. Hicks
Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1
PNAS, June 17, 2008; 105(24): 8464 - 8469.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Bainbridge, S. Guyomarc'h, E. Bayer, R. Swarup, M. Bennett, T. Mandel, and C. Kuhlemeier
Auxin influx carriers stabilize phyllotactic patterning
Genes & Dev., March 15, 2008; 22(6): 810 - 823.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Hoyerova, L. Perry, P. Hand, M. Lankova, T. Kocabek, S. May, J. Kottova, J. Paces, R. Napier, and E. Zazimalova
Functional Characterization of PaLAX1, a Putative Auxin Permease, in Heterologous Plant Systems
Plant Physiology, March 1, 2008; 146(3): 1128 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
B. B. Stone, E. L. Stowe-Evans, R. M. Harper, R. B. Celaya, K. Ljung, G. Sandberg, and E. Liscum
Disruptions in AUX1-Dependent Auxin Influx Alter Hypocotyl Phototropism in Arabidopsis
Mol Plant, January 1, 2008; 1(1): 129 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Laplaze, E. Benkova, I. Casimiro, L. Maes, S. Vanneste, R. Swarup, D. Weijers, V. Calvo, B. Parizot, M. B. Herrera-Rodriguez, et al.
Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation
PLANT CELL, December 1, 2007; 19(12): 3889 - 3900.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Cho, S. H. Lee, and H.-T. Cho
P-Glycoprotein4 Displays Auxin Efflux Transporter-Like Action in Arabidopsis Root Hair Cells and Tobacco Cells
PLANT CELL, December 1, 2007; 19(12): 3930 - 3943.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Peret, R. Swarup, L. Jansen, G. Devos, F. Auguy, M. Collin, C. Santi, V. Hocher, C. Franche, D. Bogusz, et al.
Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in Casuarina glauca
Plant Physiology, August 1, 2007; 144(4): 1852 - 1862.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Ruzicka, K. Ljung, S. Vanneste, R. Podhorska, T. Beeckman, J. Friml, and E. Benkova
Ethylene Regulates Root Growth through Effects on Auxin Biosynthesis and Transport-Dependent Auxin Distribution
PLANT CELL, July 1, 2007; 19(7): 2197 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Chhun, Y. Uno, S. Taketa, T. Azuma, M. Ichii, T. Okamoto, and S. Tsurumi
Saturated humidity accelerates lateral root development in rice (Oryza sativa L.) seedlings by increasing phloem-based auxin transport
J. Exp. Bot., May 1, 2007; 58(7): 1695 - 1704.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. J. Blakeslee, A. Bandyopadhyay, O. R. Lee, J. Mravec, B. Titapiwatanakun, M. Sauer, S. N. Makam, Y. Cheng, R. Bouchard, J. Adamec, et al.
Interactions among PIN-FORMED and P-Glycoprotein Auxin Transporters in Arabidopsis
PLANT CELL, January 1, 2007; 19(1): 131 - 147.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Orlova, A. Marshall-Colon, J. Schnepp, B. Wood, M. Varbanova, E. Fridman, J. J. Blakeslee, W. A. Peer, A. S. Murphy, D. Rhodes, et al.
Reduction of Benzenoid Synthesis in Petunia Flowers Reveals Multiple Pathways to Benzoic Acid and Enhancement in Auxin Transport
PLANT CELL, December 1, 2006; 18(12): 3458 - 3475.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Kleine-Vehn, P. Dhonukshe, R. Swarup, M. Bennett, and J. Friml
Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1
PLANT CELL, November 1, 2006; 18(11): 3171 - 3181.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Carraro, C. Forestan, S. Canova, J. Traas, and S. Varotto
ZmPIN1a and ZmPIN1b Encode Two Novel Putative Candidates for Polar Auxin Transport and Plant Architecture Determination of Maize
Plant Physiology, September 1, 2006; 142(1): 254 - 264.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. K. Muday, S. R. Brady, C. Argueso, J. Deruere, J. J. Kieber, and A. DeLong
RCN1-Regulated Phosphatase Activity and EIN2 Modulate Hypocotyl Gravitropism by a Mechanism That Does Not Require Ethylene Signaling
Plant Physiology, August 1, 2006; 141(4): 1617 - 1629.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Dharmasiri, R. Swarup, K. Mockaitis, N. Dharmasiri, S. K. Singh, M. Kowalchyk, A. Marchant, S. Mills, G. Sandberg, M. J. Bennett, et al.
AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1
Science, May 26, 2006; 312(5777): 1218 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Terasaka, J. J. Blakeslee, B. Titapiwatanakun, W. A. Peer, A. Bandyopadhyay, S. N. Makam, O. R. Lee, E. L. Richards, A. S. Murphy, F. Sato, et al.
PGP4, an ATP Binding Cassette P-Glycoprotein, Catalyzes Auxin Transport in Arabidopsis thaliana Roots
PLANT CELL, November 1, 2005; 17(11): 2922 - 2939.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Nacry, G. Canivenc, B. Muller, A. Azmi, H. Van Onckelen, M. Rossignol, and P. Doumas
A Role for Auxin Redistribution in the Responses of the Root System Architecture to Phosphate Starvation in Arabidopsis
Plant Physiology, August 1, 2005; 138(4): 2061 - 2074.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. W. WOODWARD and B. BARTEL
Auxin: Regulation, Action, and Interaction
Ann. Bot., April 1, 2005; 95(5): 707 - 735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists