This Article Full Text Full Text (PDF) All Versions of this Article: 16/12/3326    most recent tpc.104.026526v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (42) Citing Articles via Google Scholar Google Scholar Articles by Zhang, P. Articles by Aro, E.-M. Search for Related Content PubMed PubMed Citation Articles by Zhang, P. Articles by Aro, E.-M. Agricola Articles by Zhang, P. Articles by Aro, E.-M.

Expression and Functional Roles of the Two Distinct NDH-1 Complexes and the Carbon Acquisition Complex NdhD3/NdhF3/CupA/Sll1735 in Synechocystis sp PCC 6803

^a Department of Biology, Plant Physiology, and Molecular Biology, University of Turku, FIN-20014 Turku, Finland
^b Botanisches Institut, D-24098 Kiel, Germany
^c Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan

¹ To whom correspondence should be addressed. E-mail evaaro{at}utu.fi; fax 358-2-333-5549.

To investigate the (co)expression, interaction, and membrane location of multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes and their involvement in carbon acquisition, cyclic photosystem I, and respiration, we grew the wild type and specific ndh gene knockout mutants of Synechocystis sp PCC 6803 under different CO₂ and pH conditions, followed by a proteome analysis of their membrane protein complexes. Typical NDH-1 complexes were represented by NDH-1L (large) and NDH-1M (medium size), located in the thylakoid membrane. The NDH-1L complex, missing from the NdhD1/D2 mutant, was a prerequisite for photoheterotrophic growth and thus apparently involved in cellular respiration. The amount of NDH-1M and the rate of P700⁺ rereduction in darkness in the NdhD1/D2 mutant grown at low CO₂ were similar to those in the wild type, whereas in the M55 mutant (NdhB), lacking both NDH-1L and NDH-1M, the rate of P700⁺ rereduction was very slow. The NDH-1S (small) complex, localized to the thylakoid membrane and composed of only NdhD3, NdhF3, CupA, and Sll1735, was strongly induced at low CO₂ in the wild type as well as in NdhD1/D2 and M55. In contrast with the wild type and NdhD1/D2, which show normal CO₂ uptake, M55 is unable to take up CO₂ even when the NDH-1S complex is present. Conversely, the NdhD3/D4 mutant, also unable to take up CO₂, lacked NDH-1S but exhibited wild-type levels of NDH-1M at low CO₂. These results demonstrate that both NDH-1S and NDH-1M are essential for CO₂ uptake and that NDH-1M is a functional complex. We also show that the Na⁺/HCO₃^– transporter (SbtA complex) is located in the plasma membrane and is strongly induced in the wild type and mutants at low CO₂.

This article has been cited by other articles:

				K. Tsunekawa, T. Shijuku, M. Hayashimoto, Y. Kojima, K. Onai, M. Morishita, M. Ishiura, T. Kuroda, T. Nakamura, H. Kobayashi, et al. Identification and Characterization of the Na+/H+ Antiporter NhaS3 from the Thylakoid Membrane of Synechocystis sp. PCC 6803 J. Biol. Chem., June 12, 2009; 284(24): 16513 - 16521. [Abstract] [Full Text] [PDF]

				S. Ishida, A. Takabayashi, N. Ishikawa, Y. Hano, T. Endo, and F. Sato A Novel Nuclear-Encoded Protein, NDH-Dependent Cyclic Electron Flow 5, is Essential for the Accumulation of Chloroplast NAD(P)H Dehydrogenase Complexes Plant Cell Physiol., February 1, 2009; 50(2): 383 - 393. [Abstract] [Full Text] [PDF]

				S. Sirpio, Y. Allahverdiyeva, M. Holmstrom, A. Khrouchtchova, A. Haldrup, N. Battchikova, and E.-M. Aro Novel Nuclear-encoded Subunits of the Chloroplast NAD(P)H Dehydrogenase Complex J. Biol. Chem., January 9, 2009; 284(2): 905 - 912. [Abstract] [Full Text] [PDF]

				L. Peng, H. Shimizu, and T. Shikanai The Chloroplast NAD(P)H Dehydrogenase Complex Interacts with Photosystem I in Arabidopsis J. Biol. Chem., December 12, 2008; 283(50): 34873 - 34879. [Abstract] [Full Text] [PDF]

				M. Xu, G. Bernat, A. Singh, H. Mi, M. Rogner, H. B. Pakrasi, and T. Ogawa Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems Plant Cell Physiol., November 1, 2008; 49(11): 1672 - 1677. [Abstract] [Full Text] [PDF]

				T. C. Summerfield and L. A. Sherman Global Transcriptional Response of the Alkali-Tolerant Cyanobacterium Synechocystis sp. Strain PCC 6803 to a pH 10 Environment Appl. Envir. Microbiol., September 1, 2008; 74(17): 5276 - 5284. [Abstract] [Full Text] [PDF]

				M. Xu, T. Ogawa, H. B. Pakrasi, and H. Mi Identification and Localization of the CupB Protein Involved in Constitutive CO2 Uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803 Plant Cell Physiol., June 1, 2008; 49(6): 994 - 997. [Abstract] [Full Text] [PDF]

				G. D. Price, M. R. Badger, F. J. Woodger, and B. M. Long Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants J. Exp. Bot., May 1, 2008; 59(7): 1441 - 1461. [Abstract] [Full Text] [PDF]

				H. Shimizu, L. Peng, F. Myouga, R. Motohashi, K. Shinozaki, and T. Shikanai CRR23/NdhL is a Subunit of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis Plant Cell Physiol., May 1, 2008; 49(5): 835 - 842. [Abstract] [Full Text] [PDF]

				T. Nakamura, K. Naito, N. Yokota, C. Sugita, and M. Sugita A Cyanobacterial Non-coding RNA, Yfr1, is Required for Growth Under Multiple Stress Conditions Plant Cell Physiol., September 1, 2007; 48(9): 1309 - 1318. [Abstract] [Full Text] [PDF]

				M. Eisenhut, E. A. von Wobeser, L. Jonas, H. Schubert, B. W. Ibelings, H. Bauwe, H. C.P. Matthijs, and M. Hagemann Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803 Plant Physiology, August 1, 2007; 144(4): 1946 - 1959. [Abstract] [Full Text] [PDF]

				R. Muraoka, K. Okuda, Y. Kobayashi, and T. Shikanai A Eukaryotic Factor Required for Accumulation of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis Plant Physiology, December 1, 2006; 142(4): 1683 - 1689. [Abstract] [Full Text] [PDF]

				W. Ma, Y. Deng, T. Ogawa, and H. Mi Active NDH-1 Complexes from the Cyanobacterium Synechocystis sp. Strain PCC 6803 Plant Cell Physiol., October 1, 2006; 47(10): 1432 - 1436. [Abstract] [Full Text] [PDF]

				M. K. Munshi, Y. Kobayashi, and T. Shikanai CHLORORESPIRATORY REDUCTION 6 Is a Novel Factor Required for Accumulation of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis Plant Physiology, June 1, 2006; 141(2): 737 - 744. [Abstract] [Full Text] [PDF]

				J. Komenda, M. Barker, S. Kuvikova, R. de Vries, C. W. Mullineaux, M. Tichy, and P. J. Nixon The FtsH Protease slr0228 Is Important for Quality Control of Photosystem II in the Thylakoid Membrane of Synechocystis sp. PCC 6803 J. Biol. Chem., January 13, 2006; 281(2): 1145 - 1151. [Abstract] [Full Text] [PDF]

				M. R. Badger, G. D. Price, B. M. Long, and F. J. Woodger The environmental plasticity and ecological genomics of the cyanobacterial CO2 concentrating mechanism J. Exp. Bot., January 1, 2006; 57(2): 249 - 265. [Abstract] [Full Text] [PDF]

				D. Xu, X. Liu, J. Zhao, and J. Zhao FesM, a Membrane Iron-Sulfur Protein, Is Required for Cyclic Electron Flow around Photosystem I and Photoheterotrophic Growth of the Cyanobacterium Synechococcus sp. PCC 7002 Plant Physiology, July 1, 2005; 138(3): 1586 - 1595. [Abstract] [Full Text] [PDF]

				A. Blanco-Rivero, F. Leganes, E. Fernandez-Valiente, P. Calle, and F. Fernandez-Pinas mrpA, a gene with roles in resistance to Na+ and adaptation to alkaline pH in the cyanobacterium Anabaena sp. PCC7120 Microbiology, May 1, 2005; 151(5): 1671 - 1682. [Abstract] [Full Text] [PDF]