Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online November 17, 2004; 10.1105/tpc.104.023622

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
16/12/3413    most recent
tpc.104.023622v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deuschle, K.
Right arrow Articles by Frommer, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deuschle, K.
Right arrow Articles by Frommer, W. B.
Agricola
Right arrow Articles by Deuschle, K.
Right arrow Articles by Frommer, W. B.
The Plant Cell 16:3413-3425 (2004)
© 2004 American Society of Plant Biologists

The Role of {Delta}1-Pyrroline-5-Carboxylate Dehydrogenase in Proline Degradation{boxw}

Karen Deuschlea,b,1, Dietmar Funcka,1,2, Giuseppe Forlanic, Harald Stranskya, Alexander Biehld, Dario Leisterd, Eric van der Graaffe, Reinhard Kunzee and Wolf B. Frommera,b,3

a Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, D-72076 Tübingen, Germany
b Carnegie Institution, Stanford, California 94305
c Department of Biology, Università di Ferrara, 44100 Ferrara, Italy
d Max-Planck-Institut für Züchtungsforschung, 50829 Köln, Germany
e Institut für Botanik, Universität zu Köln, 50931 Köln, Germany

3 To whom correspondence should be addressed. E-mail wfrommer{at}stanford.edu; fax 650-325-6857.

In response to stress, plants accumulate Pro, requiring degradation after release from adverse conditions. {Delta}1-Pyrroline-5-carboxylate dehydrogenase (P5CDH), the second enzyme for Pro degradation, is encoded by a single gene expressed ubiquitously. To study the physiological function of P5CDH, T-DNA insertion mutants in AtP5CDH were isolated and characterized. Although Pro degradation was undetectable in p5cdh mutants, neither increased Pro levels nor an altered growth phenotype were observed under normal conditions. Thus AtP5CDH is essential for Pro degradation but not required for vegetative plant growth. External Pro application caused programmed cell death, with callose deposition, reactive oxygen species production, and DNA laddering, involving a salicylic acid signal transduction pathway. p5cdh mutants were hypersensitive toward Pro and other molecules producing P5C, such as Arg and Orn. Pro levels were the same in the wild type and mutants, but P5C was detectable only in p5cdh mutants, indicating that P5C accumulation may be the cause for Pro hypersensitivity. Accordingly, overexpression of AtP5CDH resulted in decreased sensitivity to externally supplied Pro. Thus, Pro and P5C/Glu semialdehyde may serve as a link between stress responses and cell death.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M.-G. Zhao, L. Chen, L.-L. Zhang, and W.-H. Zhang
Nitric Reductase-Dependent Nitric Oxide Production Is Involved in Cold Acclimation and Freezing Tolerance in Arabidopsis
Plant Physiology, October 1, 2009; 151(2): 755 - 767.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Miller, A. Honig, H. Stein, N. Suzuki, R. Mittler, and A. Zilberstein
Unraveling {Delta}1-Pyrroline-5-Carboxylate-Proline Cycle in Plants by Uncoupled Expression of Proline Oxidation Enzymes
J. Biol. Chem., September 25, 2009; 284(39): 26482 - 26492.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Hansen, C. Friis, S. Bowra, P. B. Holm, and E. Vincze
A pathway-specific microarray analysis highlights the complex and co-ordinated transcriptional networks of the developing grain of field-grown barley
J. Exp. Bot., January 1, 2009; 60(1): 153 - 167.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Canas, D. P. Villalobos, S. M. Diaz-Moreno, F. M. Canovas, and F. R. Canton
Molecular and Functional Analyses Support a Role of Ornithine-{delta}-Aminotransferase in the Provision of Glutamate for Glutamine Biosynthesis during Pine Germination
Plant Physiology, September 1, 2008; 148(1): 77 - 88.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Vasquez-Robinet, S. P. Mane, A. V. Ulanov, J. I. Watkinson, V. K. Stromberg, D. De Koeyer, R. Schafleitner, D. B. Willmot, M. Bonierbale, H. J. Bohnert, et al.
Physiological and molecular adaptations to drought in Andean potato genotypes
J. Exp. Bot., May 1, 2008; 59(8): 2109 - 2123.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. Bove, C. L.H. Hord, and M. A. Mullen
The blossoming of RNA biology: Novel insights from plant systems
RNA, December 1, 2006; 12(12): 2035 - 2046.
[Full Text] [PDF]

Home page
 J. Virol.Home page
D. K. Bideshi, M.-V. Demattei, F. Rouleux-Bonnin, K. Stasiak, Y. Tan, S. Bigot, Y. Bigot, and B. A. Federici
Genomic Sequence of Spodoptera frugiperda Ascovirus 1a, an Enveloped, Double-Stranded DNA Insect Virus That Manipulates Apoptosis for Viral Reproduction
J. Virol., December 1, 2006; 80(23): 11791 - 11805.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists