First published online January 16, 2004; 10.1105/tpc.019042
The Plant Cell 16:342-352 (2004)
© 2004 American Society of Plant Biologists
Interchromatid and Interhomolog Recombination in Arabidopsis thaliana
Jean Moliniera,1,
Gerhard Riesb,
Sebastian Bonhoefferc and
Barbara Hohna
a Friedrich Miescher Institute, CH-4058 Basel, Switzerland
b BioMedinvestor AG, CH-4051 Basel, Switzerland
c Department of Environmental Sciences, Eidgenössische Technische Hochschule Zürich, CH-8092 Zürich, Switzerland
1 To whom correspondence should be addressed. E-mail jean.molinier{at}fmi.ch; fax 41 61 697 39 76.
Intermolecular recombination events were monitored in Arabidopsis thaliana lines using specially designed recombination traps consisting of tandem disrupted ß-glucuronidase or luciferase reporter genes in direct repeat orientation. Recombination frequencies (RFs) varied between the different lines, indicating possible position effects influencing intermolecular recombination processes. The RFs between sister chromatids and between homologous chromosomes were measured in plants either hemizygous or homozygous for a transgene locus. The RFs in homozygous plants exceeded those of hemizygous plants by a factor of >2, implying that in somatic plant cells both sister chromatid recombination and recombination between homologous chromosomes exist for recombinational DNA repair. In addition, different DNA-damaging agents stimulated recombination in homozygous and hemizygous plants to different extents in a manner dependent on the type of DNA damage and on the genomic region. The genetic and molecular analysis of recombination events showed that most of the somatic recombination events result from gene conversion, although a pop-out event has also been characterized.
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