First published online February 18, 2004; 10.1105/tpc.019398
The Plant Cell 16:616-628 (2004)
© 2004 American Society of Plant Biologists
Plant Respiratory Burst Oxidase Homologs Impinge on Wound Responsiveness and Development in Lycopersicon esculentum
Moshe Sagia,
Olga Davydovb,
Saltanat Orazovaa,
Zhazira Yesbergenovaa,
Ron Ophirc,
Johannes W. Stratmannd and
Robert Fluhrb,1
a Institute for Applied Research, Ben-Gurion University, Beer Sheva 84105, Israel
b Department of Plant Science, Weizmann Institute of Science, Rehovot 76100, Israel
c Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
d Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208
1 To whom correspondence should be addressed. E-mail robert.fluhr{at}weizmann.ac.il; fax 972-8-9344181.
Plant respiratory burst oxidase homologs (Rboh) are homologs of the human neutrophil pathogen-related gp91phox. Antisense technology was employed to ascertain the biological function of Lycopersicon esculentum (tomato) Rboh. Lines with diminished Rboh activity showed a reduced level of reactive oxygen species (ROS) in the leaf, implying a role for Rboh in establishing the cellular redox milieu. Surprisingly, the antisense plants acquired a highly branched phenotype, switched from indeterminate to determinate growth habit, and had fasciated reproductive organs. Wound-induced systemic expression of proteinase inhibitor II was compromised in the antisense lines, indicating that ROS intermediates supplied by Rboh are required for this wound response. Extending these observations by transcriptome analysis revealed ectopic leaf expression of homeotic MADS box genes that are normally expressed only in reproductive organs. In addition, both Rboh-dependent and -independent wound-induced gene induction was detected as well as transcript changes related to redox maintenance. The results provide novel insights into how the steady state cellular level of ROS is controlled and portrays the role of Rboh as a signal transducer of stress and developmental responses.
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