The Plant Cell 16:643-657 (2004)
© 2004 American Society of Plant Biologists
Expression of a Nondegradable Cyclin B1 Affects Plant Development and Leads to Endomitosis by Inhibiting the Formation of a Phragmoplast
Magdalena Weingartnera,
Marie-Claire Criquib,
Tamás Mészárosc,
Pavla Binarovad,
Anne-Catherine Schmitb,
Anne Helferc,
Aude Derevierb,
Mathieu Erhardtb,
László Bögrec and
Pascal Genschikb,1
a Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany
b Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 67084 Strasbourg Cédex, France
c School of Biological Sciences, Royal Holloway College, University of London, Egham, Surrey TW20 OEX, United Kingdom
d Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
1 To whom correspondence should be addressed. E-mail pascal.genschik{at}ibmp-ulp.u-strasbg.fr; fax 33 (0)3 88 61 44 42.
In plants after the disassembly of mitotic spindle, a specific cytokinetic structure called the phragmoplast is built, and after cytokinesis, microtubules populate the cell cortex in an organized orientation that determines cell elongation and shape. Here, we show that impaired cyclin B1 degradation, resulting from a mutation within its destruction box, leads to an isodiametric shape of epidermal cells in leaves, stems, and roots and retarded growth of seedlings. Microtubules in these misshaped cells are grossly disorganized, focused around the nucleus, whereas they were entirely missing or abnormally organized along the cell cortex. A high percentage of cells expressing nondestructible cyclin B1 had doubled DNA content as a result of undergoing endomitosis. During anaphase the cytokinesis-specific syntaxin KNOLLE could still localize to the midplane of cell division, whereas NPK1-activating kinesin-like protein 1, a cytokinetic kinesin-related protein, was unable to do so, and instead of the formation of a phragmoplast, the midzone microtubules persisted between the separated nuclei, which eventually fused. In summary, our results show that the timely degradation of mitotic cyclins in plants is required for the reorganization of mitotic microtubules to the phragmoplast and for proper cytokinesis. Subsequently, the presence of nondegradable cyclin B1 leads to a failure in organizing properly the cortical microtubules that determine cell elongation and shape.
This article has been cited by other articles:
|
|
|
|
 
N. Riehs, S. Akimcheva, J. Puizina, P. Bulankova, R. A. Idol, J. Siroky, A. Schleiffer, D. Schweizer, D. E. Shippen, and K. Riha
Arabidopsis SMG7 protein is required for exit from meiosis
J. Cell Sci.,
July 1, 2008;
121(13):
2208 - 2216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-R. Yuan and Y. Shi
Chloramphenicol Induces Abnormal Differentiation and Inhibits Apoptosis in Activated T Cells
Cancer Res.,
June 15, 2008;
68(12):
4875 - 4881.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Serralbo, J. M. Perez-Perez, R. Heidstra, and B. Scheres
Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog
PNAS,
August 29, 2006;
103(35):
13250 - 13255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Smertenko, H.-Y. Chang, S. Sonobe, S. I. Fenyk, M. Weingartner, L. Bogre, and P. J. Hussey
Control of the AtMAP65-1 interaction with microtubules through the cell cycle
J. Cell Sci.,
August 1, 2006;
119(15):
3227 - 3237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Brukhin, J. Gheyselinck, V. Gagliardini, P. Genschik, and U. Grossniklaus
The RPN1 Subunit of the 26S Proteasome in Arabidopsis Is Essential for Embryogenesis
PLANT CELL,
October 1, 2005;
17(10):
2723 - 2737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dong, Z. Liu, Y. Zhu, F. Yu, Z. Li, K. Cao, and W.-H. Shen
Interacting Proteins and Differences in Nuclear Transport Reveal Specific Functions for the NAP1 Family Proteins in Plants
Plant Physiology,
July 1, 2005;
138(3):
1446 - 1456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
