This Article Full Text Full Text (PDF) All Versions of this Article: 16/3/709    most recent tpc.018994v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Zhou, H.-W. Articles by DeLong, A. Search for Related Content PubMed PubMed Citation Articles by Zhou, H.-W. Articles by DeLong, A. Agricola Articles by Zhou, H.-W. Articles by DeLong, A.

Disparate Roles for the Regulatory A Subunit Isoforms in Arabidopsis Protein Phosphatase 2A

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912

¹ To whom correspondence should be addressed. E-mail alison_delong{at}brown.edu; fax 401-863-2421.

The heterotrimeric protein phosphatase 2A (PP2A) complex comprises a catalytic subunit and regulatory A and B subunits that modulate enzyme activity and mediate interactions with other proteins. We report here the results of a systematic analysis of the Arabidopsis (Arabidopsis thaliana) regulatory A subunit gene family, which includes the ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1 (RCN1), PP2AA2, and PP2AA3 genes. All three A subunit isoforms accumulate in the organs of seedlings and adult plants, suggesting extensive overlap in expression domains. We have isolated pp2aa2 and pp2aa3 mutants and found that their phenotypes are largely normal and do not resemble that of rcn1. Whereas rcn1 pp2aa2 and rcn1 pp2aa3 double mutants exhibit striking abnormalities in all stages of development, the pp2aa2 pp2aa3 double mutant shows only modest defects. Together, these data suggest that RCN1 performs a cardinal role in regulation of phosphatase activity and that PP2AA2 and PP2AA3 functions are unmasked only when RCN1 is absent.

This article has been cited by other articles:

				P. Sukumar, K. S. Edwards, A. Rahman, A. DeLong, and G. K. Muday PINOID Kinase Regulates Root Gravitropism through Modulation of PIN2-Dependent Basipetal Auxin Transport in Arabidopsis Plant Physiology, June 1, 2009; 150(2): 722 - 735. [Abstract] [Full Text] [PDF]

				J. J. Blakeslee, H.-W. Zhou, J. T. Heath, K. R. Skottke, J. A. R. Barrios, S.-Y. Liu, and A. DeLong Specificity of RCN1-Mediated Protein Phosphatase 2A Regulation in Meristem Organization and Stress Response in Roots Plant Physiology, February 1, 2008; 146(2): 539 - 553. [Abstract] [Full Text] [PDF]

				Y. Zhu, A. Dong, D. Meyer, O. Pichon, J.-P. Renou, K. Cao, and W.-H. Shen Arabidopsis NRP1 and NRP2 Encode Histone Chaperones and Are Required for Maintaining Postembryonic Root Growth PLANT CELL, November 1, 2006; 18(11): 2879 - 2892. [Abstract] [Full Text] [PDF]

				G. K. Muday, S. R. Brady, C. Argueso, J. Deruere, J. J. Kieber, and A. DeLong RCN1-Regulated Phosphatase Activity and EIN2 Modulate Hypocotyl Gravitropism by a Mechanism That Does Not Require Ethylene Signaling Plant Physiology, August 1, 2006; 141(4): 1617 - 1629. [Abstract] [Full Text] [PDF]