First published online February 18, 2004; 10.1105/tpc.019661
The Plant Cell 16:723-730 (2004)
© 2004 American Society of Plant Biologists
Growth Regulators and the Control of Nucleotide Sugar Flux
Georg J. Seifert,
Christine Barber,
Brian Wells and
Keith Roberts1
Department of Cell and Developmental Biology, John Innes Center, Colney, Norwich NR4 7UH, United Kingdom
1 To whom correspondence should be addressed. E-mail keith.roberts{at}bbsrc.ac.uk; fax 44-1603-450019.
A small number of plant growth regulators are involved in the control of cell expansion. Despite knowledge of some of their signal transduction cascades, surprisingly little is known of how basic cell expansion–related processes, such as cell wall biosynthesis, are affected during growth. The Arabidopsis (Arabidopsis thaliana) mutant root hair defective1 (rhd1) lacks a functional UDP-glucose 4-epimerase gene, UGE4, which is involved in channeling UDP-D-galactose (UDP-D-Gal) into cell wall polymers. Here, we use rhd1 as a genetic model to analyze the physiological and genetic controls of nucleotide sugar flux. We find that ethylene specifically suppresses all visible aspects of the rhd1 phenotype. The ethylene-triggered suppression of rhd1 is negatively regulated by CONSTITUTIVE TRIPLE RESPONSE1 and requires the function of the wild-type genes ETHYLENE INSENSITIVE2 (EIN2), EIN4, AUXIN-RESISTENT1, and ETHYLENE-INSENSITIVE ROOT1 but does not depend on the activity of wild-type ETHYLENE RECEPTOR1 or EIN3 genes, highlighting the nonlinearity of ethylene signal transduction. Ethylene does not induce the expression of alternative UGE genes but, instead, suppresses the expression of two isoforms, UGE1 and UGE3, in a tissue-specific manner. Ethylene restores the biosynthesis of galactose-containing xyloglucan and arabinosylated galactan cell wall polymers in rhd1 back to wild-type levels. However, the dependence on UGE4 of pectic (1 4)-ß-D-galactan and glucuronosyl-modified AGP biosynthesis is exacerbated. Our data suggest that ethylene and auxin together participate in the flux control of UDP-D-Gal into cell wall polymers and that the genetic control of this process is qualitatively distinct from previously described responses to ethylene.
This article has been cited by other articles:
|
|
|
|
 
D. M. Cavalier, O. Lerouxel, L. Neumetzler, K. Yamauchi, A. Reinecke, G. Freshour, O. A. Zabotina, M. G. Hahn, I. Burgert, M. Pauly, et al.
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component
PLANT CELL,
June 1, 2008;
20(6):
1519 - 1537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Rosti, C. J. Barton, S. Albrecht, P. Dupree, M. Pauly, K. Findlay, K. Roberts, and G. J. Seifert
UDP-Glucose 4-Epimerase Isoforms UGE2 and UGE4 Cooperate in Providing UDP-Galactose for Cell Wall Biosynthesis and Growth of Arabidopsis thaliana
PLANT CELL,
May 1, 2007;
19(5):
1565 - 1579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogawa, H. Uchimiya, and M. Kawai-Yamada
Mutual Regulation of Arabidopsis thaliana Ethylene-responsive Element Binding Protein and a Plant Floral Homeotic Gene, APETALA2
Ann. Bot.,
February 1, 2007;
99(2):
239 - 244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Barber, J. Rosti, A. Rawat, K. Findlay, K. Roberts, and G. J. Seifert
Distinct Properties of the Five UDP-D-glucose/UDP-D-galactose 4-Epimerase Isoforms of Arabidopsis thaliana
J. Biol. Chem.,
June 23, 2006;
281(25):
17276 - 17285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Nguema-Ona, C. Andeme-Onzighi, S. Aboughe-Angone, M. Bardor, T. Ishii, P. Lerouge, and A. Driouich
The reb1-1 Mutation of Arabidopsis. Effect on the Structure and Localization of Galactose-Containing Cell Wall Polysaccharides
Plant Physiology,
April 1, 2006;
140(4):
1406 - 1417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Shi, S.-W. Zhu, X.-Z. Mao, J.-X. Feng, Y.-M. Qin, L. Zhang, J. Cheng, L.-P. Wei, Z.-Y. Wang, and Y.-X. Zhu
Transcriptome Profiling, Molecular Biological, and Physiological Studies Reveal a Major Role for Ethylene in Cotton Fiber Cell Elongation
PLANT CELL,
March 1, 2006;
18(3):
651 - 664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ringli, N. Baumberger, and B. Keller
The Arabidopsis Root Hair Mutants der2-der9 are Affected at Different Stages of Root Hair Development
Plant Cell Physiol.,
July 1, 2005;
46(7):
1046 - 1053.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vicre, C. Santaella, S. Blanchet, A. Gateau, and A. Driouich
Root Border-Like Cells of Arabidopsis. Microscopical Characterization and Role in the Interaction with Rhizobacteria
Plant Physiology,
June 1, 2005;
138(2):
998 - 1008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Teixeira, C. Knorpp, and K. Glimelius
Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus
J. Exp. Bot.,
April 1, 2005;
56(414):
1245 - 1253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
