First published online March 12, 2004; 10.1105/tpc.017947
The Plant Cell 16:857-873 (2004)
© 2004 American Society of Plant Biologists
Purification and Characterization of Serine Proteases That Exhibit Caspase-Like Activity and Are Associated with Programmed Cell Death in Avena sativa
Warren C. Coffeen and
Thomas J. Wolpert1
Department of Botany and Plant Pathology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon, 97331-2902
1 To whom correspondence should be addressed. E-mail wolpertt{at}bcc.orst.edu; fax 541-737-3573.
Victoria blight of Avena sativa (oat) is caused by the fungus Cochliobolus victoriae, which is pathogenic because of the production of the toxin victorin. The victorin-induced response in sensitive A. sativa has been characterized as a form of programmed cell death (PCD) and displays morphological and biochemical features similar to apoptosis, including chromatin condensation, DNA laddering, cell shrinkage, altered mitochondrial function, and ordered, substrate-specific proteolytic events. Victorin-induced proteolysis of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is shown to be prevented by caspase-specific and general protease inhibitors. Evidence is presented for a signaling cascade leading to Rubisco proteolysis that involves multiple proteases. Furthermore, two proteases that are apparently involved in the Rubisco proteolytic cascade were purified and characterized. These proteases exhibit caspase specificity and display amino acid sequences homologous to plant subtilisin-like Ser proteases. The proteases are constitutively present in an active form and are relocalized to the extracellular fluid after induction of PCD by either victorin or heat shock. The role of the enzymes as processive proteases involved in a signal cascade during the PCD response is discussed.
This article has been cited by other articles:
|
|
|
|
 
A. Figueiredo, A. M. Fortes, S. Ferreira, M. Sebastiana, Y. H. Choi, L. Sousa, B. Acioli-Santos, F. Pessoa, R. Verpoorte, and M. S. Pais
Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi
J. Exp. Bot.,
September 1, 2008;
59(12):
3371 - 3381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Desclos, L. Dubousset, P. Etienne, F. Le Caherec, H. Satoh, J. Bonnefoy, A. Ourry, and J.-C. Avice
A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions
Plant Physiology,
August 1, 2008;
147(4):
1830 - 1844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. P. van Esse, J. W. van't Klooster, M. D. Bolton, K. A. Yadeta, P. van Baarlen, S. Boeren, J. Vervoort, P. J.G.M. de Wit, and B. P.H.J. Thomma
The Cladosporium fulvum Virulence Protein Avr2 Inhibits Host Proteases Required for Basal Defense
PLANT CELL,
July 1, 2008;
20(7):
1948 - 1963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, G. Brader, and E. T. Palva
Kunitz Trypsin Inhibitor: An Antagonist of Cell Death Triggered by Phytopathogens and Fumonisin B1 in Arabidopsis
Mol Plant,
May 1, 2008;
1(3):
482 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. G. van Doorn and E. J. Woltering
Physiology and molecular biology of petal senescence
J. Exp. Bot.,
March 3, 2008;
(2008)
erm356v2.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bonneau, Y. Ge, G. E. Drury, and P. Gallois
What happened to plant caspases?
J. Exp. Bot.,
February 13, 2008;
(2008)
erm352v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Kingston-Smith, T. E. Davies, J. E. Edwards, and M. K. Theodorou
From plants to animals; the role of plant cell death in ruminant herbivores
J. Exp. Bot.,
February 4, 2008;
(2008)
erm326v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bosch and V. E. Franklin-Tong
Self-incompatibility in Papaver: signalling to trigger PCD in incompatible pollen
J. Exp. Bot.,
February 1, 2008;
59(3):
481 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Hong, B.-W. Yun, J.-G. Kang, M. U. Raja, E. Kwon, K. Sorhagen, C. Chu, Y. Wang, and G. J. Loake
Nitric oxide function and signalling in plant disease resistance
J. Exp. Bot.,
February 1, 2008;
59(2):
147 - 154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bosch and V. E. Franklin-Tong
Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen
PNAS,
November 13, 2007;
104(46):
18327 - 18332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Vercammen, W. Declercq, P. Vandenabeele, and F. Van Breusegem
Are metacaspases caspases?
J. Cell Biol.,
November 5, 2007;
179(3):
375 - 380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Muntz
Protein dynamics and proteolysis in plant vacuoles
J. Exp. Bot.,
July 1, 2007;
58(10):
2391 - 2407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zuppini, C. Andreoli, and B. Baldan
Heat Stress: an Inducer of Programmed Cell Death in Chlorella saccharophila
Plant Cell Physiol.,
July 1, 2007;
48(7):
1000 - 1009.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Sweat and T. J. Wolpert
Thioredoxin h5 Is Required for Victorin Sensitivity Mediated by a CC-NBS-LRR Gene in Arabidopsis
PLANT CELL,
February 1, 2007;
19(2):
673 - 687.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boren, A.-S. Hoglund, P. Bozhkov, and C. Jansson
Developmental regulation of a VEIDase caspase-like proteolytic activity in barley caryopsis
J. Exp. Bot.,
November 1, 2006;
57(14):
3747 - 3753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Milleron and S. B. Bratton
Heat Shock Induces Apoptosis Independently of Any Known Initiator Caspase-activating Complex
J. Biol. Chem.,
June 23, 2006;
281(25):
16991 - 17000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Radwan, S. Mouzeyar, J. S. Venisse, P. Nicolas, and M. F. Bouzidi
Resistance of sunflower to the biotrophic oomycete Plasmopara halstedii is associated with a delayed hypersensitive response within the hypocotyls
J. Exp. Bot.,
October 1, 2005;
56(420):
2683 - 2693.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kuroyanagi, K. Yamada, N. Hatsugai, M. Kondo, M. Nishimura, and I. Hara-Nishimura
Vacuolar Processing Enzyme Is Essential for Mycotoxin-induced Cell Death in Arabidopsis thaliana
J. Biol. Chem.,
September 23, 2005;
280(38):
32914 - 32920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Watanabe and E. Lam
Two Arabidopsis Metacaspases AtMCP1b and AtMCP2b Are Arginine/Lysine-specific Cysteine Proteases and Activate Apoptosis-like Cell Death in Yeast
J. Biol. Chem.,
April 15, 2005;
280(15):
14691 - 14699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sanmartin, L. Jaroszewski, N. V. Raikhel, and E. Rojo
Caspases. Regulating Death Since the Origin of Life
Plant Physiology,
March 1, 2005;
137(3):
841 - 847.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Vercammen, B. van de Cotte, G. De Jaeger, D. Eeckhout, P. Casteels, K. Vandepoele, I. Vandenberghe, J. Van Beeumen, D. Inze, and F. Van Breusegem
Type II Metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana Cleave Substrates after Arginine and Lysine
J. Biol. Chem.,
October 29, 2004;
279(44):
45329 - 45336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tian, E. Huitema, L. da Cunha, T. Torto-Alalibo, and S. Kamoun
A Kazal-like Extracellular Serine Protease Inhibitor from Phytophthora infestans Targets the Tomato Pathogenesis-related Protease P69B
J. Biol. Chem.,
June 18, 2004;
279(25):
26370 - 26377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
