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First published online April 20, 2004; 10.1105/tpc.018929

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The Plant Cell 16:1276-1287 (2004)
© 2004 American Society of Plant Biologists

Extracellular Invertase Is an Essential Component of Cytokinin-Mediated Delay of Senescence

Maria Encarnación Balibrea Laraa, Maria-Cruz Gonzalez Garciaa, Tahira Fatimab, Rainer Ehneßb, Taek Kyun Leeb, Reinhard Proelsa, Widmar Tannerb and Thomas Roitscha,1

a Lehrstuhl für Pharmazeutische Biologie, Julius von Sachs Institut, Universität Würzburg, D-97082 Würzburg, Germany
b Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, D-93040 Regensburg, Germany

1 To whom correspondence should be addressed. E-mail roitsch{at}biozentrum.uni-wuerzburg.de; fax 49-931-888-6181.

Leaf senescence is the final stage of leaf development in which the nutrients invested in the leaf are remobilized to other parts of the plant. Whereas senescence is accompanied by a decline in leaf cytokinin content, exogenous application of cytokinins or an increase of the endogenous concentration delays senescence and causes nutrient mobilization. The finding that extracellular invertase and hexose transporters, as the functionally linked enzymes of an apolasmic phloem unloading pathway, are coinduced by cytokinins suggested that delay of senescence is mediated via an effect on source-sink relations. This hypothesis was further substantiated in this study by the finding that delay of senescence in transgenic tobacco (Nicotiana tabacum) plants with autoregulated cytokinin production correlates with an elevated extracellular invertase activity. The finding that the expression of an extracellular invertase under control of the senescence-induced SAG12 promoter results in a delay of senescence demonstrates that effect of cytokinins may be substituted by these metabolic enzymes. The observation that an increase in extracellular invertase is sufficient to delay leaf senescence was further verified by a complementing functional approach. Localized induction of an extracellular invertase under control of a chemically inducible promoter resulted in ectopic delay of senescence, resembling the naturally occurring green islands in autumn leaves. To establish a causal relationship between cytokinins and extracellular invertase for the delay of senescence, transgenic plants were generated that allowed inhibition of extracellular invertase in the presence of cytokinins. For this purpose, an invertase inhibitor was expressed under control of a cytokinin-inducible promoter. It has been shown that senescence is not any more delayed by cytokinin when the expression of the invertase inhibitor is elevated. This finding demonstrates that extracellular invertase is required for the delay of senescence by cytokinins and that it is a key element of the underlying molecular mechanism.




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