Plant Cell Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online April 20, 2004; 10.1105/tpc.020487

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
16/5/1327    most recent
tpc.020487v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (77)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hussain, D.
Right arrow Articles by Cobbett, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hussain, D.
Right arrow Articles by Cobbett, C. S.
Agricola
Right arrow Articles by Hussain, D.
Right arrow Articles by Cobbett, C. S.
The Plant Cell 16:1327-1339 (2004)
© 2004 American Society of Plant Biologists

P-Type ATPase Heavy Metal Transporters with Roles in Essential Zinc Homeostasis in Arabidopsis

Dawar Hussaina, Michael J. Haydona, Yuwen Wangb, Edwin Wonga, Sarah M. Shersona, Jeff Youngb, James Camakarisa, Jeffrey F. Harperb and Christopher S. Cobbetta,1

a Department of Genetics, University of Melbourne, Australia 3010
b Department of Cell Biology, Plant Division, Scripps Research Institute, La Jolla, California, 92037

1 To whom correspondence should be addressed. E-mail ccobbett{at}unimelb.edu.au; fax 61-3-83445138.

Arabidopsis thaliana has eight genes encoding members of the type 1B heavy metal–transporting subfamily of the P-type ATPases. Three of these transporters, HMA2, HMA3, and HMA4, are closely related to each other and are most similar in sequence to the divalent heavy metal cation transporters of prokaryotes. To determine the function of these transporters in metal homeostasis, we have identified and characterized mutants affected in each. Whereas the individual mutants exhibited no apparent phenotype, hma2 hma4 double mutants had a nutritional deficiency phenotype that could be compensated for by increasing the level of Zn, but not Cu or Co, in the growth medium. Levels of Zn, but not other essential elements, in the shoot tissues of a hma2 hma4 double mutant and, to a lesser extent, of a hma4 single mutant were decreased compared with the wild type. Together, these observations indicate a primary role for HMA2 and HMA4 in essential Zn homeostasis. HMA2promoter- and HMA4promoter-reporter gene constructs provide evidence that HMA2 and HMA4 expression is predominantly in the vascular tissues of roots, stems, and leaves. In addition, expression of the genes in developing anthers was confirmed by RT-PCR and was consistent with a male-sterile phenotype in the double mutant. HMA2 appears to be localized to the plasma membrane, as indicated by protein gel blot analysis of membrane fractions using isoform-specific antibodies and by the visualization of an HMA2-green fluorescent protein fusion by confocal microscopy. These observations are consistent with a role for HMA2 and HMA4 in Zn translocation. hma2 and hma4 mutations both conferred increased sensitivity to Cd in a phytochelatin-deficient mutant background, suggesting that they may also influence Cd detoxification.




This article has been cited by other articles:


Home page
 J Exp BotHome page
H. Yamaguchi, H. Fukuoka, T. Arao, A. Ohyama, T. Nunome, K. Miyatake, and S. Negoro
Gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, Solanum torvum
J. Exp. Bot., January 1, 2010; 61(2): 423 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Ueno, E. Koyama, I. Kono, T. Ando, M. Yano, and J. F. Ma
Identification of a Novel Major Quantitative Trait Locus Controlling Distribution of Cd Between Roots and Shoots in Rice
Plant Cell Physiol., December 1, 2009; 50(12): 2223 - 2233.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Kawachi, Y. Kobae, H. Mori, R. Tomioka, Y. Lee, and M. Maeshima
A Mutant Strain Arabidopsis thaliana that Lacks Vacuolar Membrane Zinc Transporter MTP1 Revealed the Latent Tolerance to Excessive Zinc
Plant Cell Physiol., June 1, 2009; 50(6): 1156 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. K. Grennan
Identification of Genes Involved in Metal Transport in Plants
Plant Physiology, April 1, 2009; 149(4): 1623 - 1624.
[Full Text] [PDF]

Home page
 J Exp BotHome page
B. Tauris, S. Borg, P. L. Gregersen, and P. B. Holm
A roadmap for zinc trafficking in the developing barley grain based on laser capture microdissection and gene expression profiling
J. Exp. Bot., March 1, 2009; 60(4): 1333 - 1347.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Morel, J. Crouzet, A. Gravot, P. Auroy, N. Leonhardt, A. Vavasseur, and P. Richaud
AtHMA3, a P1B-ATPase Allowing Cd/Zn/Co/Pb Vacuolar Storage in Arabidopsis
Plant Physiology, February 1, 2009; 149(2): 894 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Kobayashi, K. Kuroda, K. Kimura, J. L. Southron-Francis, A. Furuzawa, K. Kimura, S. Iuchi, M. Kobayashi, G. J. Taylor, and H. Koyama
Amino Acid Polymorphisms in Strictly Conserved Domains of a P-Type ATPase HMA5 Are Involved in the Mechanism of Copper Tolerance Variation in Arabidopsis
Plant Physiology, October 1, 2008; 148(2): 969 - 980.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Klein, H. Sekimoto, M. J. Milner, and L. V. Kochian
Investigation of Heavy Metal Hyperaccumulation at the Cellular Level: Development and Characterization of Thlaspi caerulescens Suspension Cell Lines
Plant Physiology, August 1, 2008; 147(4): 2006 - 2016.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. J. Milner and L. V. Kochian
Investigating Heavy-metal Hyperaccumulation using Thlaspi caerulescens as a Model System
Ann. Bot., July 1, 2008; 102(1): 3 - 13.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Ueno, T. Iwashita, F.-J. Zhao, and J. F. Ma
Characterization of Cd Translocation and Identification of the Cd Form in Xylem Sap of the Cd-Hyperaccumulator Arabidopsis halleri
Plant Cell Physiol., April 1, 2008; 49(4): 540 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Lee, Y.-Y. Kim, Y. Lee, and G. An
Rice P1B-Type Heavy-Metal ATPase, OsHMA9, Is a Metal Efflux Protein
Plant Physiology, November 1, 2007; 145(3): 831 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Yuan, D. Loque, S. Kojima, S. Rauch, K. Ishiyama, E. Inoue, H. Takahashi, and N. von Wiren
The Organization of High-Affinity Ammonium Uptake in Arabidopsis Roots Depends on the Spatial Arrangement and Biochemical Properties of AMT1-Type Transporters
PLANT CELL, August 1, 2007; 19(8): 2636 - 2652.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Courbot, G. Willems, P. Motte, S. Arvidsson, N. Roosens, P. Saumitou-Laprade, and N. Verbruggen
A Major Quantitative Trait Locus for Cadmium Tolerance in Arabidopsis halleri Colocalizes with HMA4, a Gene Encoding a Heavy Metal ATPase
Plant Physiology, June 1, 2007; 144(2): 1052 - 1065.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Peiter, B. Montanini, A. Gobert, P. Pedas, S. Husted, F. J. M. Maathuis, D. Blaudez, M. Chalot, and D. Sanders
A secretory pathway-localized cation diffusion facilitator confers plant manganese tolerance
PNAS, May 15, 2007; 104(20): 8532 - 8537.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. Willems, D. B. Drager, M. Courbot, C. Gode, N. Verbruggen, and P. Saumitou-Laprade
The Genetic Basis of Zinc Tolerance in the Metallophyte Arabidopsis halleri ssp. halleri (Brassicaceae): An Analysis of Quantitative Trait Loci
Genetics, May 1, 2007; 176(1): 659 - 674.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Haydon and C. S. Cobbett
A Novel Major Facilitator Superfamily Protein at the Tonoplast Influences Zinc Tolerance and Accumulation in Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1705 - 1719.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. Adle, D. Sinani, H. Kim, and J. Lee
A Cadmium-transporting P1B-type ATPase in Yeast Saccharomyces cerevisiae
J. Biol. Chem., January 12, 2007; 282(2): 947 - 955.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Eren, D. C. Kennedy, M. J. Maroney, and J. M. Arguello
A Novel Regulatory Metal Binding Domain Is Present in the C Terminus of Arabidopsis Zn2+-ATPase HMA2
J. Biol. Chem., November 10, 2006; 281(45): 33881 - 33891.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. E. van de Mortel, L. Almar Villanueva, H. Schat, J. Kwekkeboom, S. Coughlan, P. D. Moerland, E. Ver Loren van Themaat, M. Koornneef, and M. G.M. Aarts
Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens
Plant Physiology, November 1, 2006; 142(3): 1127 - 1147.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. N. Talke, M. Hanikenne, and U. Kramer
Zinc-Dependent Global Transcriptional Control, Transcriptional Deregulation, and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri
Plant Physiology, September 1, 2006; 142(1): 148 - 167.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Seigneurin-Berny, A. Gravot, P. Auroy, C. Mazard, A. Kraut, G. Finazzi, D. Grunwald, F. Rappaport, A. Vavasseur, J. Joyard, et al.
HMA1, a New Cu-ATPase of the Chloro plast Envelope, Is Essential for Growth under Adverse Light Conditions
J. Biol. Chem., February 3, 2006; 281(5): 2882 - 2892.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N.-H. Cheng, J. K. Pittman, T. Shigaki, J. Lachmansingh, S. LeClere, B. Lahner, D. E. Salt, and K. D. Hirschi
Functional Association of Arabidopsis CAX1 and CAX3 Is Required for Normal Growth and Ion Homeostasis
Plant Physiology, August 1, 2005; 138(4): 2048 - 2060.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. E. Abdel-Ghany, P. Muller-Moule, K. K. Niyogi, M. Pilon, and T. Shikanai
Two P-Type ATPases Are Required for Copper Delivery in Arabidopsis thaliana Chloroplasts
PLANT CELL, April 1, 2005; 17(4): 1233 - 1251.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Hanikenne, U. Kramer, V. Demoulin, and D. Baurain
A Comparative Inventory of Metal Transporters in the Green Alga Chlamydomonas reinhardtii and the Red Alga Cyanidioschizon merolae
Plant Physiology, February 1, 2005; 137(2): 428 - 446.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Eren and J. M. Arguello
Arabidopsis HMA2, a Divalent Heavy Metal-Transporting PIB-Type ATPase, Is Involved in Cytoplasmic Zn2+ Homeostasis
Plant Physiology, November 1, 2004; 136(3): 3712 - 3723.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Papoyan and L. V. Kochian
Identification of Thlaspi caerulescens Genes That May Be Involved in Heavy Metal Hyperaccumulation and Tolerance. Characterization of a Novel Heavy Metal Transporting ATPase
Plant Physiology, November 1, 2004; 136(3): 3814 - 3823.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. E. Sondergaard, A. Schulz, and M. G. Palmgren
Energization of Transport Processes in Plants. Roles of the Plasma Membrane H+-ATPase
Plant Physiology, September 1, 2004; 136(1): 2475 - 2482.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists