Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online April 20, 2004; 10.1105/tpc.020743

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
16/5/1340    most recent
tpc.020743v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lewandowska, D.
Right arrow Articles by Jarmolowski, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewandowska, D.
Right arrow Articles by Jarmolowski, A.
Agricola
Right arrow Articles by Lewandowska, D.
Right arrow Articles by Jarmolowski, A.
The Plant Cell 16:1340-1352 (2004)
© 2004 American Society of Plant Biologists

Determinants of Plant U12-Dependent Intron Splicing Efficiency

Dominika Lewandowskaa,1,2, Craig G. Simpsonb,1, Gillian P. Clarkb, Nikki S. Jenningsb, Maria Barciszewska-Pacaka, Chiao-Feng Linc, Wojciech Makalowskic, John W.S. Brownb,3 and Artur Jarmolowskia

a Department of Gene Expression, Adam Mickiewicz University, Poznan 60-371, Poland
b Gene Expression Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom
c Institute of Molecular Evolutionary Genetics and Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802

3 To whom correspondence should be addressed. E-mail jbrown{at}scri.sari.ac.uk; fax 44-1382-562426.

Factors affecting splicing of plant U12-dependent introns have been examined by extensive mutational analyses in an in vivo tobacco (Nicotiana tabacum) protoplast system using introns from three different Arabidopsis thaliana genes: CBP20, GSH2, and LD. The results provide evidence that splicing efficiency of plant U12 introns depends on a combination of factors, including UA content, exon bridging interactions between the U12 intron and flanking U2-dependent introns, and exon splicing enhancer sequences (ESEs). Unexpectedly, all three plant U12 introns required an adenosine at the upstream purine position in the branchpoint consensus UCCUURAUY. The exon upstream of the LD U12 intron is a major determinant of its higher level of splicing efficiency and potentially contains two ESE regions. These results suggest that in plants, U12 introns represent a level at which expression of their host genes can be regulated.




This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Li, X. Li, L. Guo, F. Lu, X. Feng, K. He, L. Wei, Z. Chen, L.-J. Qu, and H. Gu
A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice
J. Exp. Bot., March 1, 2006; 57(6): 1263 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Z. J. LORKOVIC, R. LEHNER, C. FORSTNER, and A. BARTA
Evolutionary conservation of minor U12-type spliceosome between plants and humans
RNA, July 1, 2005; 11(7): 1095 - 1107.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists