First published online May 21, 2004; 10.1105/tpc.020677
The Plant Cell 16:1564-1574 (2004)
© 2004 American Society of Plant Biologists
Woronin Body Function in Magnaporthe grisea Is Essential for Efficient Pathogenesis and for Survival during Nitrogen Starvation Stress
Shanthi Soundararajana,1,
Gregory Jeddb,1,
Xiaolei Lia,
Marilou Ramos-Pamploñaa,c,
Nam H. Chuab and
Naweed I. Naqvia,c,2
a Fungal Genomics Laboratory, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604
b Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New York 10021
c Department of Biological Sciences, National University of Singapore, Singapore 117543
2 To whom correspondence should be addressed. E-mail naweed{at}tll.org.sg; fax 65-6872-7007.
The Woronin body is a peroxisome-derived dense-core vesicle that is specific to several genera of filamentous ascomycetes, where it has been shown to seal septal pores in response to cellular damage. The Hexagonal peroxisome (Hex1) protein was recently identified as a major constituent of the Woronin body and shown to be responsible for self-assembly of the dense core of this organelle. Using a mutation in the Magnaporthe grisea HEX1 ortholog, we define a dual and essential function for Woronin bodies during the pathogenic phase of the rice blast fungus. We show that the Woronin body is initially required for proper development and function of appressoria (infection structures) and subsequently necessary for survival of infectious fungal hyphae during invasive growth and host colonization. Fungal mycelia lacking HEX1 function were unable to survive nitrogen starvation in vitro, suggesting that in planta growth defects are a consequence of the mutant's inability to cope with nutritional stress. Thus, Woronin body function provides the blast fungus with an important defense against the antagonistic and nutrient-limiting environment encountered within the host plant.
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