This Article Full Text Full Text (PDF) All Versions of this Article: 16/9/2307    most recent tpc.104.024919v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (55) Citing Articles via Google Scholar Google Scholar Articles by Qiao, H. Articles by Xue, Y. Search for Related Content PubMed PubMed Citation Articles by Qiao, H. Articles by Xue, Y. Agricola Articles by Qiao, H. Articles by Xue, Y.

The F-Box Protein AhSLF-S₂ Controls the Pollen Function of S-RNase–Based Self-Incompatibility

^a Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
^b Plant Science Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, United Kingdom

² To whom correspondence should be addressed. E-mail ybxue{at}genetics.ac.cn; fax 86-10-6253-7814.

Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S₂ plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone (TAC26) containing both AhSLF-S₂ and AhS₂-RNase into a self-incompatible (SI) line of Petunia hybrida. Molecular analyses showed that both genes are correctly expressed in pollen and pistil in four independent transgenic lines of petunia. Pollination tests indicated that all four lines became self-compatible because of the specific loss of the pollen function of SI. This alteration was transmitted stably into the T1 progeny. We then transformed AhSLF-S₂ cDNA under the control of a tomato (Lycopersicon esculentum) pollen-specific promoter LAT52 into the self-incompatible petunia line. Molecular studies revealed that AhSLF-S₂ is specifically expressed in pollen of five independent transgenic plants. Pollination tests showed that they also had lost the pollen function of SI. Importantly, expression of endogenous SLF or SLF-like genes was not altered in these transgenic plants. These results phenocopy a well-known phenomenon called competitive interaction whereby the presence of two different pollen S alleles within pollen leads to the breakdown of the pollen function of SI in several solanaceaous species. Furthermore, we demonstrated that AhSLF-S₂ physically interacts with PhS₃-RNase from the P. hybrida line used for transformation. Together with the recent demonstration of PiSLF as the pollen determinant in P. inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S₂ controls the pollen function of S-RNase–based self-incompatibility.

This article has been cited by other articles:

				M. J. Wheeler, S. Vatovec, and V. E. Franklin-Tong The pollen S-determinant in Papaver: comparisons with known plant receptors and protein ligand partners J. Exp. Bot., January 22, 2010; (2010): erp383v1 - erp383. [Abstract] [Full Text] [PDF]

				G. Chen, B. Zhang, Z. Zhao, Z. Sui, H. Zhang, and Y. Xue 'A life or death decision' for pollen tubes in S-RNase-based self-incompatibility J. Exp. Bot., December 30, 2009; (2009) erp381v1. [Abstract] [Full Text] [PDF]

				B. McClure Darwin's foundation for investigating self-incompatibility and the progress toward a physiological model for S-RNase-based SI J. Exp. Bot., March 1, 2009; 60(4): 1069 - 1081. [Abstract] [Full Text] [PDF]

				H. Qiao, K. N. Chang, J. Yazaki, and J. R. Ecker Interplay between ethylene, ETP1/ETP2 F-box proteins, and degradation of EIN2 triggers ethylene responses in Arabidopsis Genes & Dev., February 15, 2009; 23(4): 512 - 521. [Abstract] [Full Text] [PDF]

				Y. Zhang, W. Xu, Z. Li, X. W. Deng, W. Wu, and Y. Xue F-Box Protein DOR Functions As a Novel Inhibitory Factor for Abscisic Acid-Induced Stomatal Closure under Drought Stress in Arabidopsis Plant Physiology, December 1, 2008; 148(4): 2121 - 2133. [Abstract] [Full Text] [PDF]

				X. Yang, U. C. Kalluri, S. Jawdy, L. E. Gunter, T. Yin, T. J. Tschaplinski, D. J. Weston, P. Ranjan, and G. A. Tuskan The F-Box Gene Family Is Expanded in Herbaceous Annual Plants Relative to Woody Perennial Plants Plant Physiology, November 1, 2008; 148(3): 1189 - 1200. [Abstract] [Full Text] [PDF]

				W.-Q. Yang, Y. Lai, M.-N. Li, W.-Y. Xu, and Y.-B. Xue A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice Mol Plant, September 1, 2008; 1(5): 770 - 785. [Abstract] [Full Text] [PDF]

				Z.-H. Hua, A. Fields, and T.-h. Kao Biochemical Models for S-RNase-Based Self-Incompatibility Mol Plant, July 1, 2008; 1(4): 575 - 585. [Abstract] [Full Text] [PDF]

				D. Wheeler and E. Newbigin Expression of 10 S-Class SLF-like Genes in Nicotiana alata Pollen and Its Implications for Understanding the Pollen Factor of the S Locus Genetics, December 1, 2007; 177(4): 2171 - 2180. [Abstract] [Full Text] [PDF]

				Z. Hua, X. Meng, and T.-h. Kao Comparison of Petunia inflata S-Locus F-Box Protein (Pi SLF) with Pi SLF Like Proteins Reveals Its Unique Function in S-RNase Based Self-Incompatibility PLANT CELL, November 1, 2007; 19(11): 3593 - 3609. [Abstract] [Full Text] [PDF]

				M. Jain, A. Nijhawan, R. Arora, P. Agarwal, S. Ray, P. Sharma, S. Kapoor, A. K. Tyagi, and J. P. Khurana F-Box Proteins in Rice. Genome-Wide Analysis, Classification, Temporal and Spatial Gene Expression during Panicle and Seed Development, and Regulation by Light and Abiotic Stress Plant Physiology, April 1, 2007; 143(4): 1467 - 1483. [Abstract] [Full Text] [PDF]

				S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen Mol. Cell. Proteomics, February 1, 2007; 6(2): 207 - 230. [Abstract] [Full Text] [PDF]

				H. Chen, V. J. Karplus, H. Ma, and X. W. Deng Plant Biology Research Comes of Age in China PLANT CELL, November 1, 2006; 18(11): 2855 - 2864. [Full Text] [PDF]

				Z. Hua and T.-h. Kao Identification and Characterization of Components of a Putative Petunia S-Locus F-Box-Containing E3 Ligase Complex Involved in S-RNase-Based Self-Incompatibility PLANT CELL, October 1, 2006; 18(10): 2531 - 2553. [Abstract] [Full Text] [PDF]

				Z. Kong, M. Li, W. Yang, W. Xu, and Y. Xue A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice Plant Physiology, August 1, 2006; 141(4): 1376 - 1388. [Abstract] [Full Text] [PDF]

				X. Qin, B. Liu, J. Soulard, D. Morse, and M. Cappadocia Style-by-style analysis of two sporadic self-compatible Solanum chacoense lines supports a primary role for S-RNases in determining pollen rejection thresholds J. Exp. Bot., June 1, 2006; 57(9): 2001 - 2013. [Abstract] [Full Text] [PDF]

				T. Sonneveld, K. R. Tobutt, S. P. Vaughan, and T. P. Robbins Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype-Specific F-Box Gene PLANT CELL, January 1, 2005; 17(1): 37 - 51. [Abstract] [Full Text] [PDF]

				J. Moon, G. Parry, and M. Estelle The Ubiquitin-Proteasome Pathway and Plant Development PLANT CELL, December 1, 2004; 16(12): 3181 - 3195. [Full Text] [PDF]

				B. McClure S-RNase and SLF Determine S-Haplotype-Specific Pollen Recognition and Rejection PLANT CELL, November 1, 2004; 16(11): 2840 - 2847. [Full Text] [PDF]