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First published online December 17, 2004; 10.1105/tpc.104.027821

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The Plant Cell 17:164-181 (2005)
© 2005 American Society of Plant Biologists

Two Plant–Viral Movement Proteins Traffic in the Endocytic Recycling Pathway{boxw}

Sophie Haupta, Graham H. Cowanb, Angelika Zieglerb, Alison G. Robertsa, Karl J. Oparkaa and Lesley Torranceb,1

a Programme of Cell-to-Cell Communication, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
b Programme of Plant–Pathogen Interactions, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom

1 To whom correspondence should be addressed. E-mail ltorra{at}scri.sari.ac.uk; fax 44-1382-568578.

Many plant viruses exploit a conserved group of proteins known as the triple gene block (TGB) for cell-to-cell movement. Here, we investigated the interaction of two TGB proteins (TGB2 and TGB3) of Potato mop-top virus (PMTV), with components of the secretory and endocytic pathways when expressed as N-terminal fusions to green fluorescent protein or monomeric red fluorescent protein (mRFP). Our studies revealed that fluorophore-labeled TGB2 and TGB3 showed an early association with the endoplasmic reticulum (ER) and colocalized in motile granules that used the ER-actin network for intracellular movement. Both proteins increased the size exclusion limit of plasmodesmata, and TGB3 accumulated at plasmodesmata in the absence of TGB2. TGB3 contains a putative Tyr-based sorting motif, mutations in which abolished ER localization and plasmodesmatal targeting. Later in the expression cycle, both fusion proteins were incorporated into vesicular structures. TGB2 associated with these structures on its own, but TGB3 could not be incorporated into the vesicles in the absence of TGB2. Moreover, in addition to localization to the ER and motile granules, mRFP-TGB3 was incorporated into vesicles when expressed in PMTV-infected epidermal cells, indicating recruitment by virus-expressed TGB2. The TGB fusion protein-containing vesicles were labeled with FM4-64, a marker for plasma membrane internalization and components of the endocytic pathway. TGB2 also colocalized in vesicles with Ara7, a Rab5 ortholog that marks the early endosome. Protein interaction analysis revealed that recombinant TGB2 interacted with a tobacco protein belonging to the highly conserved RME-8 family of J-domain chaperones, shown to be essential for endocytic trafficking in Caenorhabditis elegans and Drosophila melanogaster. Collectively, the data indicate the involvement of the endocytic pathway in viral intracellular movement, the implications of which are discussed.




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