This Article Full Text Full Text (PDF) All Versions of this Article: 17/1/256    most recent tpc.104.027557v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (49) Citing Articles via Google Scholar Google Scholar Articles by Gong, Z. Articles by Zhu, J.-K. Search for Related Content PubMed PubMed Citation Articles by Gong, Z. Articles by Zhu, J.-K. Agricola Articles by Gong, Z. Articles by Zhu, J.-K.

A DEAD Box RNA Helicase Is Essential for mRNA Export and Important for Development and Stress Responses in Arabidopsis

^a State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
^b Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721
^c Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521

¹ To whom correspondence should be addressed. E-mail jian-kang.zhu{at}ucr.edu; fax 951-827-7115.

An Arabidopsis thaliana mutant, cryophyte, was isolated and found to have an enhanced cold stress-induction of the master regulator of cold tolerance, C-repeat binding factor 2 (CBF2), and its downstream target genes. The mutant is more tolerant to chilling and freezing stresses but is more sensitive to heat stress. Under warm but not cold growth temperatures, the mutant has a reduced stature and flowers earlier. Under long day conditions, flowering of the mutant is insensitive to vernalization. The mutant is also hypersensitive to the phytohormone abscisic acid. The mutation was found in a DEAD box RNA helicase gene that is identical to the previously identified low expression of osmotically responsive genes 4 (LOS4) locus, which was defined by the los4-1 mutation that reduces cold regulation of CBFs and their target genes and renders Arabidopsis plants chilling sensitive. We show evidence suggesting that the CRYOPHYTE/LOS4 protein may be enriched in the nuclear rim. In situ poly(A) hybridization indicates that the export of poly(A)⁺ RNAs is blocked in the cryophyte/los4-2 mutant at warm or high temperatures but not at low temperatures, whereas the los4-1 mutation weakens mRNA export at both low and warm temperatures. These results demonstrate an important role of the CRYOPHYTE/LOS4 RNA helicase in mRNA export, plant development, and stress responses.

This article has been cited by other articles:

				J. S. Kim, K. A. Kim, T. R. Oh, C. M. Park, and H. Kang Functional Characterization of DEAD-Box RNA Helicases in Arabidopsis thaliana under Abiotic Stress Conditions Plant Cell Physiol., October 1, 2008; 49(10): 1563 - 1571. [Abstract] [Full Text] [PDF]

				J. C. Cuevas, R. Lopez-Cobollo, R. Alcazar, X. Zarza, C. Koncz, T. Altabella, J. Salinas, A. F. Tiburcio, and A. Ferrando Putrescine Is Involved in Arabidopsis Freezing Tolerance and Cold Acclimation by Regulating Abscisic Acid Levels in Response to Low Temperature Plant Physiology, October 1, 2008; 148(2): 1094 - 1105. [Abstract] [Full Text] [PDF]

				M. Badawi, Y. V. Reddy, Z. Agharbaoui, Y. Tominaga, J. Danyluk, F. Sarhan, and M. Houde Structure and Functional Analysis of Wheat ICE (Inducer of CBF Expression) Genes Plant Cell Physiol., August 1, 2008; 49(8): 1237 - 1249. [Abstract] [Full Text] [PDF]

				D. Li, H. Liu, H. Zhang, X. Wang, and F. Song OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress J. Exp. Bot., May 1, 2008; 59(8): 2133 - 2146. [Abstract] [Full Text] [PDF]

				H. H. Liu, J. Liu, S. L. Fan, M. Z. Song, X. L. Han, F. Liu, and F. F. Shen Molecular cloning and characterization of a salinity stress-induced gene encoding DEAD-box helicase from the halophyte Apocynum venetum J. Exp. Bot., February 13, 2008; (2008) erm355v1. [Abstract] [Full Text] [PDF]

				P. Kant, S. Kant, M. Gordon, R. Shaked, and S. Barak STRESS RESPONSE SUPPRESSOR1 and STRESS RESPONSE SUPPRESSOR2, Two DEAD-Box RNA Helicases That Attenuate Arabidopsis Responses to Multiple Abiotic Stresses Plant Physiology, November 1, 2007; 145(3): 814 - 830. [Abstract] [Full Text] [PDF]

				J. Sun, H. Jiang, Y. Xu, H. Li, X. Wu, Q. Xie, and C. Li The CCCH-Type Zinc Finger Proteins AtSZF1 and AtSZF2 Regulate Salt Stress Responses in Arabidopsis Plant Cell Physiol., August 1, 2007; 48(8): 1148 - 1158. [Abstract] [Full Text] [PDF]

				X. M. Xu, A. Rose, S. Muthuswamy, S. Y. Jeong, S. Venkatakrishnan, Q. Zhao, and I. Meier NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development PLANT CELL, May 1, 2007; 19(5): 1537 - 1548. [Abstract] [Full Text] [PDF]

				C.-H. Dong, X. Hu, W. Tang, X. Zheng, Y. S. Kim, B.-h. Lee, and J.-K. Zhu A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for RNA Export and Required for Plant Tolerance to Cold Stress Mol. Cell. Biol., December 15, 2006; 26(24): 9533 - 9543. [Abstract] [Full Text] [PDF]

				Z. Chen, H. Zhang, D. Jablonowski, X. Zhou, X. Ren, X. Hong, R. Schaffrath, J.-K. Zhu, and Z. Gong Mutations in ABO1/ELO2, a Subunit of Holo-Elongator, Increase Abscisic Acid Sensitivity and Drought Tolerance in Arabidopsis thaliana. Mol. Cell. Biol., September 1, 2006; 26(18): 6902 - 6912. [Abstract] [Full Text] [PDF]

				M. A. Hannah, D. Wiese, S. Freund, O. Fiehn, A. G. Heyer, and D. K. Hincha Natural Genetic Variation of Freezing Tolerance in Arabidopsis Plant Physiology, September 1, 2006; 142(1): 98 - 112. [Abstract] [Full Text] [PDF]

				G. Parry, S. Ward, A. Cernac, S. Dharmasiri, and M. Estelle The Arabidopsis SUPPRESSOR OF AUXIN RESISTANCE Proteins Are Nucleoporins with an Important Role in Hormone Signaling and Development PLANT CELL, July 1, 2006; 18(7): 1590 - 1603. [Abstract] [Full Text] [PDF]

				G. W. Owttrim RNA helicases and abiotic stress Nucleic Acids Res., June 21, 2006; 34(11): 3220 - 3230. [Abstract] [Full Text] [PDF]

				W. Van de Velde, J. C. P. Guerra, A. D. Keyser, R. De Rycke, S. Rombauts, N. Maunoury, P. Mergaert, E. Kondorosi, M. Holsters, and S. Goormachtig Aging in Legume Symbiosis. A Molecular View on Nodule Senescence in Medicago truncatula Plant Physiology, June 1, 2006; 141(2): 711 - 720. [Abstract] [Full Text] [PDF]

				B.-B. Wang and V. Brendel Molecular Characterization and Phylogeny of U2AF35 Homologs in Plants Plant Physiology, February 1, 2006; 140(2): 624 - 636. [Abstract] [Full Text] [PDF]

				B.-h. Lee, D. A. Henderson, and J.-K. Zhu The Arabidopsis Cold-Responsive Transcriptome and Its Regulation by ICE1 PLANT CELL, November 1, 2005; 17(11): 3155 - 3175. [Abstract] [Full Text] [PDF]