First published online November 11, 2005; 10.1105/tpc.105.036640
The Plant Cell 17:3500-3512 (2005)
© 2005 American Society of Plant Biologists
PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 Is a Plant-Specific SEC12-Related Protein That Enables the Endoplasmic Reticulum Exit of a High-Affinity Phosphate Transporter in Arabidopsis[W]
Esperanza González,
Roberto Solano,
Vicente Rubio,
Antonio Leyva and
Javier Paz-Ares1
Centro Nacional de Biotecnología–Consejo Superior de Investigaciones Científicas, Campus de Cantoblanco, Madrid E-28049, Spain
1 To whom correspondence should be addressed. E-mail jpazares{at}cnb.uam.es; fax 34-91585-4506.
PHOSPHATE TRANSPORTER1 (PHT1) genes encode phosphate (Pi) transporters that play a fundamental role in Pi acquisition and remobilization in plants. Mutation of the Arabidopsis thaliana PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) impairs Pi transport, resulting in the constitutive expression of many Pi starvation–induced genes, increased arsenate resistance, and reduced Pi accumulation. PHF1 expression was detected in all tissues, particularly in roots, flowers, and senescing leaves, and was induced by Pi starvation, thus mimicking the expression patterns of the whole PHT1 gene family. PHF1 was localized in endoplasmic reticulum (ER), and mutation of PHF1 resulted in ER retention and reduced accumulation of the plasma membrane PHT1;1 transporter. By contrast, the PIP2A plasma membrane protein was not mislocalized, and the secretion of Pi starvation–induced RNases was not affected in the mutant. PHF1 encodes a plant-specific protein structurally related to the SEC12 proteins of the early secretory pathway. However, PHF1 lacks most of the conserved residues in SEC12 proteins essential as guanine nucleotide exchange factors. Although it functions in early secretory trafficking, PHF1 likely evolved a novel mechanism accompanying functional specialization on Pi transporters. The identification of PHF1 reveals that plants are also endowed with accessory proteins specific for selected plasma membrane proteins, allowing their exit from the ER, and that these ER exit cofactors may have a phylum-specific origin.
This article has been cited by other articles:
|
|
|
|
 
J. P. Hammond and P. J. White
Sucrose transport in the phloem: integrating root responses to phosphorus starvation
J. Exp. Bot.,
January 1, 2008;
59(1):
93 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. N. Devaiah, V. K. Nagarajan, and K. G. Raghothama
Phosphate Homeostasis and Root Development in Arabidopsis Are Synchronized by the Zinc Finger Transcription Factor ZAT6
Plant Physiology,
September 1, 2007;
145(1):
147 - 159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem.,
August 10, 2007;
282(32):
23541 - 23552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Miller, X. Fan, M. Orsel, S. J. Smith, and D. M. Wells
Nitrate transport and signalling
J. Exp. Bot.,
July 1, 2007;
58(9):
2297 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Catarecha, M. D. Segura, J. M. Franco-Zorrilla, B. Garcia-Ponce, M. Lanza, R. Solano, J. Paz-Ares, and A. Leyva
A Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic Accumulation
PLANT CELL,
March 1, 2007;
19(3):
1123 - 1133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Muller, M. Morant, H. Jarmer, L. Nilsson, and T. H. Nielsen
Genome-Wide Analysis of the Arabidopsis Leaf Transcriptome Reveals Interaction of Phosphate and Sugar Metabolism
Plant Physiology,
January 1, 2007;
143(1):
156 - 171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Orsel, F. Chopin, O. Leleu, S. J. Smith, A. Krapp, F. Daniel-Vedele, and A. J. Miller
Characterization of a Two-Component High-Affinity Nitrate Uptake System in Arabidopsis. Physiology and Protein-Protein Interaction
Plant Physiology,
November 1, 2006;
142(3):
1304 - 1317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Aung, S.-I Lin, C.-C. Wu, Y.-T. Huang, C.-l. Su, and T.-J. Chiou
pho2, a Phosphate Overaccumulator, Is Caused by a Nonsense Mutation in a MicroRNA399 Target Gene
Plant Physiology,
July 1, 2006;
141(3):
1000 - 1011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dharmasiri, R. Swarup, K. Mockaitis, N. Dharmasiri, S. K. Singh, M. Kowalchyk, A. Marchant, S. Mills, G. Sandberg, M. J. Bennett, et al.
AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1
Science,
May 26, 2006;
312(5777):
1218 - 1220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Eckardt
Insights into Plant Cellular Mechanisms: Of Phosphate Transporters and Arbuscular Mycorrhizal Infection
PLANT CELL,
December 1, 2005;
17(12):
3213 - 3216.
[Full Text]
[PDF]
|
|
|
|
|
